Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,950,984	C→A	6.1%	R658L (CGT→CTT)	ppk ←	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,950,984	0	C	A	6.1%	208.3 / 3.7	65	R658L (CGT→CTT)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (27/34);  new base A (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCG  >  NC_002947/5950839‑5951125                                                                                                                                                  |                                                                                                                                              cTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGc                                                                                                                                             <  2:102254/148‑1 (MQ=255)    gTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTg                                                                                                                                          <  3:211053/148‑1 (MQ=255)    gTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGt                                                                                                                                         >  6:211586/1‑149 (MQ=255)     ttGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTc                                                                                                                                        <  5:211586/149‑1 (MQ=255)         cGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAgg                                                                                                                                    <  2:25141/149‑1 (MQ=255)          ggTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCAGCGGGAATCAGGTCTCGACAAGCGTGTCGAgac                                                                                                                                   <  6:125728/149‑3 (MQ=255)          ggTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGt                                                                                                                                   <  5:106042/149‑1 (MQ=255)          ggTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGT‑CAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGCCTCGACAAGCGTGTCGAgacc                                                                                                                                  <  2:262734/149‑4 (MQ=255)           gTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGGCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGtt                                                                                                                                  <  1:226656/149‑1 (MQ=255)                 gTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtc                                                                                                                            <  2:116019/149‑1 (MQ=255)                   gctgcGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtcca                                                                                                                          <  7:191540/149‑1 (MQ=255)                     tgcGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCTCCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatc                                                                                                                        >  4:179559/1‑149 (MQ=255)                            gTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCgg                                                                                                                 <  4:161782/149‑1 (MQ=255)                                   ccGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCTCCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGa                                                                                                          <  3:179559/149‑1 (MQ=255)                                           cTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAgg                                                                                                      >  5:172113/1‑145 (MQ=255)                                            tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAgg                                                                                                                                    <  5:244648/114‑1 (MQ=255)                                            tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAgg                                                                                                                                    >  6:244648/1‑114 (MQ=255)                                            tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCAGCAGCAACAGTTTCTTGCCTTCCACCTGGAACCAGGTCTCGACACCCTTGTCGACGATGCGCTCCATCCAGTCGGCGATGGACAgg                                                                                                      >  8:60246/1‑144 (MQ=255)                                               aaGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTg                                                                                              >  5:345349/1‑149 (MQ=255)                                                     cAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTc                                                                                        >  8:126166/1‑149 (MQ=255)                                                              gTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgcc                                                                                     <  3:245745/143‑1 (MQ=255)                                                              gTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgcc                                                                                     >  4:245745/1‑143 (MQ=255)                                                                 ttgggggTCAGGTAGACTTCCAGCTCCTTCTTCACGCGCAGGATGAGTTTGTTGCCTTCGACCGGGAAGCAGGTCTCGACTCGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGTCAGGTAGTTCTGCTCTTCGCCGCCATTGa                                                                             <  7:60246/143‑1 (MQ=255)                                                                 ttGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTCCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAg                                                                            <  4:247112/149‑1 (MQ=255)                                                                 ttGGCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGGGGTCCATCCAGTCGGCGCTGGTCTGGTAGATCTGCTCTTCGCCGCCATTgt                                                                             <  8:87482/148‑2 (MQ=255)                                                                  tGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAg                                                                            <  6:345349/148‑1 (MQ=255)                                                                   gTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGgc                                                                                                                >  6:225066/1‑111 (MQ=255)                                                                   gTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGgc                                                                                                                <  5:225066/111‑1 (MQ=255)                                                                   gTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAgg                                                                           <  1:306655/148‑1 (MQ=255)                                                                      ggTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcg                                                                                       <  8:176517/133‑1 (MQ=255)                                                                      ggTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcg                                                                                       >  7:176517/1‑133 (MQ=255)                                                                      ggTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAg                                                                        >  3:284931/1‑148 (MQ=255)                                                                       gTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAg                                                                        >  1:327988/1‑147 (MQ=255)                                                                          aGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGaa                                                                   >  4:104110/1‑149 (MQ=255)                                                                          aGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGaa                                                                   <  3:104110/149‑1 (MQ=255)                                                                           ggTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAac                                                                  <  6:172113/149‑1 (MQ=255)                                                                            gTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAaca                                                                 >  8:104926/1‑149 (MQ=255)                                                                                ccTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatcca                                                                                                                      <  4:257280/92‑1 (MQ=255)                                                                                ccTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatcca                                                                                                                      >  3:257280/1‑92 (MQ=255)                                                                                    ccAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCgt                                                          <  7:81183/148‑1 (MQ=255)                                                                                          ccTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTc                                                    >  4:93830/1‑148 (MQ=255)                                                                                           cttcttCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTc                                                    >  6:312803/1‑147 (MQ=255)                                                                                                   cccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgccg                                                                                    >  3:183532/1‑107 (MQ=255)                                                                                                   cccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcgccg                                                                                    <  4:183532/107‑1 (MQ=255)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGa                                                  <  8:211432/140‑1 (MQ=255)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGa                                                  >  7:211432/1‑140 (MQ=255)                                                                                                         gcaACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATg                                    <  7:104926/149‑1 (MQ=255)                                                                                                          caACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGa                                   <  7:126166/149‑1 (MQ=255)                                                                                                              aGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGa                               <  3:93830/149‑1 (MQ=255)                                                                                                              aGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGa                               <  2:49673/149‑1 (MQ=255)                                                                                                                tttCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAgc                             >  6:285035/1‑149 (MQ=255)                                                                                                                tttCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAgc                             <  2:327988/149‑1 (MQ=255)                                                                                                                         ttCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCAc                         <  6:148680/144‑1 (MQ=255)                                                                                                                         ttCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCAc                         >  5:148680/1‑144 (MQ=255)                                                                                                                         ttCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTACTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGa                    >  1:151373/1‑149 (MQ=255)                                                                                                                             aCCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCAc                         >  3:302894/1‑140 (MQ=255)                                                                                                                             aCCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCAc                         <  4:302894/140‑1 (MQ=255)                                                                                                                               cGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttgt              <  7:46344/149‑1 (MQ=255)                                                                                                                                  gAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGc            >  2:184282/1‑148 (MQ=255)                                                                                                                                  gAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCg           <  1:184282/149‑1 (MQ=255)                                                                                                                                        aacctcGACAAGCGTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAccc     >  4:294071/4‑149 (MQ=255)                                                                                                                                          tctcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGCTCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAccccc   >  6:305584/1‑149 (MQ=255)                                                                                                                                          tctcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAccccc   >  4:321667/1‑149 (MQ=255)                                                                                                                                          cctcGACAAGCGTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAAccc     >  8:339601/2‑147 (MQ=255)                                                                                                                                           ctcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCg  >  5:133616/1‑149 (MQ=255)                                                                                                                                                 acGCTTTTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAg                                                                     <  8:20423/76‑1 (MQ=255)                                                                                                                                                 acGCTTGTCTAGGTTGCGCTCCATCCAGTAGGCGCTGGACAGGTAGATCTGCTCTTAGCCGCCATTGAGGAAGTAg                                                                     >  7:20423/1‑76 (MQ=255)                                                                                                                                                  |                                                                                                                                              CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCG  >  NC_002947/5950839‑5951125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑