Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,952,898 A→G 8.3% V20A (GTC→GCC)  ppk ← polyphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,952,8980AG 8.3% 125.9 / 2.7 48V20A (GTC→GCC) ppkpolyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

GGGTAGTTCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGC  >  NC_002947/5952754‑5953041
                                                                                                                                                |                                                                                                                                               
gggTCGTTCGAGTCCCGTGCCGGCTCCTGCACGCGGATCTTTTAGTGCAGTGGCGAGAGTTCGCGATGACTGTACAGGCTGCTGTCGTCGAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGaccac                                                                                                                                             <  4:290896/149‑1 (MQ=255)
    aGTTCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGt                                                                                                                                         >  4:41202/1‑149 (MQ=255)
      ttCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTc                                                                                                                                       <  1:206637/149‑1 (MQ=255)
      ttCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTc                                                                                                                                       >  8:214178/1‑149 (MQ=255)
      ttCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTc                                                                                                                                       <  7:194323/149‑1 (MQ=255)
         tcGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGc                                                                                                                                    >  6:53236/1‑149 (MQ=255)
                   ccTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGc                                                                                                                             <  4:29657/146‑1 (MQ=255)
                   ccTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGc                                                                                                                             >  3:29657/1‑146 (MQ=255)
                            gCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGa                                                                                                                 <  7:214178/149‑1 (MQ=255)
                               gccGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGATGCTGTCGTCCAGGCCTGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGGCTCGAGCACCTGTTCCGCTACAGGCTCGGCCAGCGGAGGc                                                                                                              <  6:331934/148‑1 (MQ=255)
                                ccGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAgg                                                                                                               >  3:221813/1‑147 (MQ=255)
                                 cGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAg                                                                                                            <  1:285246/149‑1 (MQ=255)
                                           aCTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCggg                                                                                                  <  6:147694/149‑1 (MQ=255)
                                                      gggagTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCAt                                                                                       >  8:300071/3‑149 (MQ=255)
                                                           ttCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATctct                                                                                   >  4:175224/1‑148 (MQ=255)
                                                             cgcgATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCtcttc                                                                                 <  5:53236/148‑1 (MQ=255)
                                                                gATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTggg                                                                             <  4:221813/149‑1 (MQ=255)
                                                                gATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTggg                                                                             <  7:300071/149‑1 (MQ=255)
                                                                     aTGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGGCCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTc                                                                        >  5:125972/1‑149 (MQ=255)
                                                                          cAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGa                                                                    <  3:41202/148‑1 (MQ=255)
                                                                          cAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGa                                                                    <  5:345186/148‑1 (MQ=255)
                                                                             gctgctGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCAtc                                                                                      >  8:117303/1‑127 (MQ=255)
                                                                             gctgctGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCAtc                                                                                      <  7:117303/127‑1 (MQ=255)
                                                                                    tcgtcCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGAt                                                         >  1:123153/1‑149 (MQ=255)
                                                                                           ggccaggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGa                                                                            >  8:26684/1‑123 (MQ=255)
                                                                                           ggccaggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGa                                                                            <  7:26684/123‑1 (MQ=255)
                                                                                              caggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGCGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTGCTGGGAGCTCCTGAGCAGCCGTGATCGCGACCggg                                               >  8:167300/1‑149 (MQ=255)
                                                                                              caggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGGCCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAggg                                               <  6:125972/149‑1 (MQ=255)
                                                                                              caggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGa                                                                    <  2:59905/128‑1 (MQ=255)
                                                                                              caggGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGa                                                                    >  1:59905/1‑128 (MQ=255)
                                                                                                 ggACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAgg                                                >  2:325657/1‑145 (MQ=255)
                                                                                                 ggACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAgg                                                <  1:325657/145‑1 (MQ=255)
                                                                                                  gACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTa                                           <  6:365775/149‑1 (MQ=255)
                                                                                                   aCGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGGCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAg                                          <  7:167300/149‑1 (MQ=255)
                                                                                                       cGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACt                                      <  3:175224/149‑1 (MQ=255)
                                                                                                            gTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCat                                  <  2:123153/148‑1 (MQ=255)
                                                                                                            gTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTCTTCAGCTACACGATCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGCGAGCTCCTGAGCATCATTGATCGCGACAGGGCTTAGCACTTCat                                  >  6:267709/1‑148 (MQ=255)
                                                                                                                gggACGGCGCGGGCACCGGAGCCACAGCCTCGGCCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCa                                   <  3:313757/143‑1 (MQ=255)
                                                                                                                gggACGGCGCGGGCACCGGAGCCACAGCCTCGGCCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCa                                   >  4:313757/1‑143 (MQ=255)
                                                                                                                                  gAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACtt                                     <  7:261792/123‑1 (MQ=255)
                                                                                                                                  gAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACtt                                     >  8:261792/1‑123 (MQ=255)
                                                                                                                                  gAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGa           >  3:284062/1‑149 (MQ=255)
                                                                                                                                     ccACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGCTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCATGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGt        >  7:80530/1‑149 (MQ=255)
                                                                                                                                       acaGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGtt       >  7:333096/1‑148 (MQ=255)
                                                                                                                                       acaGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGtt       >  8:139458/1‑148 (MQ=255)
                                                                                                                                       acaGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGtt       >  2:188767/1‑148 (MQ=255)
                                                                                                                                       acaGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGAAGtt       >  5:345133/1‑148 (MQ=255)
                                                                                                                                       acaGCCTCGACCACCTGTTCCGCTACAGGATCGGCCACCGGAGGCAGGTCTGGAGGGGTCTGCAGGATCTCGTCTTGGAGCTACTGAGCTTCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATGTGGCGTTGCTGAGGACGtt       >  6:173641/1‑148 (MQ=255)
                                                                                                                                           ccTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTGCTGAGCATCCTTGATCGCGACAGGGCTTAGCCCTTCAGTATTAATCTGGCGTTCCTGAGGCCGTTAACGc  >  8:231402/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GGGTAGTTCTCGTCCAGTGCCTGCTCCAGCACCCGGATATTGAACTGCAGTTGCGAGAGTTCGCGATGAATGTACAGGCTGCTGTCGTCCAGGCCAGGGACGGCGATGGTCGGGGACGGCGCGGGCACCGGAGCCACAGCCTCGACCACCTGTTCCGCTACAGGCTCGGCCACCGGAGGCAGGTCTGGCGGGGTCTGCAGCATCTCTTCTGGGAGCTCCTGAGCATCCTTGATCGCGACAGGGCTTAGCACTTCATTATTCATCTGGCGTTCCTGAGGACGTTAACGC  >  NC_002947/5952754‑5953041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑