Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,956,336	A→G	7.4%	V44A (GTG→GCG)	PP_5221 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,956,336	0	A	G	7.4%	141.4 / 2.9	56	V44A (GTG→GCG)	PP_5221	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/29);  new base G (2/2);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

CGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCCATCATGCCTGAAGCGCAGCGA  >  NC_002947/5956189‑5956482                                                                                                                                                    |                                                                                                                                                    cGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGccac                                                                                                                                                    <  2:85109/149‑1 (MQ=255)   gTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGccacc                                                                                                                                                   >  6:207218/1‑148 (MQ=255)      ggCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATca                                                                                                                                               <  7:30373/149‑1 (MQ=255)          aGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCAGCTTGGCCACCGCCTGCCCCGCCACCATcaccac                                                                                                                                           >  1:313718/1‑149 (MQ=255)                 cGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGTCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AAc                                                                                                                                   <  8:31167/149‑1 (MQ=255)                 cGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGGCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AAc                                                                                                                                   <  1:7070/149‑1 (MQ=255)                     aCTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTgc                                                                                                                               <  4:170254/149‑1 (MQ=255)                            aTCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATcac                                                                                                                                              >  8:167486/1‑128 (MQ=255)                            aTCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATcac                                                                                                                                              <  7:167486/128‑1 (MQ=255)                                   gCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCTGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAAc                                                                                                                 <  2:313718/149‑1 (MQ=255)                                   gCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAAc                                                                                                                 <  5:211751/149‑1 (MQ=255)                                   gCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAAc                                                                                                                 >  6:65970/1‑149 (MQ=255)                                       gCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGa                                                                                                             <  7:230048/149‑1 (MQ=255)                                        cccGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGAc                                                                                                            <  3:29604/149‑1 (MQ=255)                                        cccGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTCCGCTTGAGGGCGAACTTGAc                                                                                                            <  2:229007/149‑1 (MQ=255)                                         ccGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGAc                                                                                                            >  5:22330/1‑148 (MQ=255)                                           ggCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGCTCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCTCCTCG‑GACGTGCGCTTGAGGGCGAACTGGACGAt                                                                                                         >  4:283620/1‑149 (MQ=255)                                           ggCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGAt                                                                                                         >  6:318217/1‑149 (MQ=255)                                            gCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCCCCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGAt                                                                                                         >  2:279061/1‑148 (MQ=255)                                            gCACGGCGTGCTCCACAATGTAGGCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATc                                                                                                        <  2:335190/149‑1 (MQ=255)                                             cACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCa                                                                                                       <  5:65970/149‑1 (MQ=255)                                               cGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGa                                                                                                     <  2:242987/149‑1 (MQ=255)                                                        ccACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCGCCATCACCACCTCG‑AACTTGCGCTTGa                                                                                                                         <  3:305240/120‑1 (MQ=255)                                                        ccACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCGCCATCACCACCTCG‑AACTTGCGCTTGa                                                                                                                         >  8:30609/1‑120 (MQ=255)                                                        ccACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCGCCATCACCACCTCG‑AACTTGCGCTTGa                                                                                                                         <  7:30609/120‑1 (MQ=255)                                                        ccACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCGCCATCACCACATCG‑AACTTGCGCTTGa                                                                                                                         >  4:305240/1‑120 (MQ=255)                                                         cacaATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACCATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCGCC‑AACTTGCGCTTGACGGCGAACTTGACGATCAGATCCTTGgcct                                                                                           >  4:42116/1‑148 (MQ=255)                                                             aTGTAGTCGTCGGCCGCATCCATTCCGCTGGGAACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACAGCCTGCCCAGCAAACATAACAACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATAACATCCTTAGCCGCGtt                                                                                       >  6:124559/1‑149 (MQ=255)                                                                tAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTgg                                                                                     <  3:351488/148‑1 (MQ=255)                                                                   tcgtcgGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGa                                                                                                             <  1:326134/121‑1 (MQ=255)                                                                   tcgtcgGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGa                                                                                                             >  2:326134/1‑121 (MQ=255)                                                                    cgtcgGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCTCCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAg                                                                                >  5:195471/1‑149 (MQ=255)                                                                        ggCCGCATCCCTGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCGGCCCCGCCACCATCACCACCTCGCCACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCg                                                                             <  1:279061/149‑1 (MQ=255)                                                                        ggCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGt                                                                            >  7:208118/1‑149 (MQ=255)                                                                          ccGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCg                                                                          >  1:11183/1‑149 (MQ=255)                                                                          ccGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCg                                                                          >  7:25209/1‑149 (MQ=255)                                                                              aTCCATGCAGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCATGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCAt                                                                      >  5:207616/1‑149 (MQ=255)                                                                               tCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATc                                                                     <  6:22330/149‑1 (MQ=255)                                                                                  aTGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGAt                                                                  <  4:211574/149‑1 (MQ=255)                                                                                       gCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATa                                                             <  5:318217/149‑1 (MQ=255)                                                                                             caccacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTgg                                                                                     >  5:199047/1‑119 (MQ=255)                                                                                             caccacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTgg                                                                                     <  6:199047/119‑1 (MQ=255)                                                                                              accacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCAt                                                      >  5:59442/1‑149 (MQ=255)                                                                                              accacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCAt                                                      >  8:216967/1‑149 (MQ=255)                                                                                               ccacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCa                                                                       >  8:129498/1‑131 (MQ=255)                                                                                               ccacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCa                                                                       <  7:129498/131‑1 (MQ=255)                                                                                                cacGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCATGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTg                                                    <  6:207616/149‑1 (MQ=255)                                                                                                   gATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCAtt                                                     <  3:232700/145‑1 (MQ=255)                                                                                                   gATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCAtt                                                     >  4:232700/1‑145 (MQ=255)                                                                                                       aGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCTCCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACAct                                              <  6:195471/148‑1 (MQ=255)                                                                                                          cgcACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACActctc                                           >  6:158290/1‑148 (MQ=255)                                                                                                              cGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACAt                                      >  6:301644/1‑149 (MQ=255)                                                                                                                   gcgAACGCCGGGTTGGCCTCCGCATGCGCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACTATCAGATCATTGGCCGCTTTGGGGCAGGGGTCGGCATCGATCCATACTCTCATTGGACACTCTCTACATGAAc                                  <  5:124559/148‑1 (MQ=255)                                                                                                                       aCGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAAt                             <  3:42116/149‑1 (MQ=255)                                                                                                                           cGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATgcgc                         <  5:207218/149‑1 (MQ=255)                                                                                                                              cTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCca                      <  8:208118/149‑1 (MQ=255)                                                                                                                                 ggCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCcat                     <  6:59442/147‑1 (MQ=255)                                                                                                                                     aCCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCAGCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCCATCATGcc               <  5:158290/149‑1 (MQ=255)                                                                                                                                                    accaTCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCCATCATGCCTGAAGCGCAGCGa  >  5:319530/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCG‑AACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGGACACTCTCTACATGAACTCAATGCGCCCATCATGCCTGAAGCGCAGCGA  >  NC_002947/5956189‑5956482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑