BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,984,862	G→A	94.7%	P86P (CCC→CCT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,984,862	0	G	A	94.7%	3400.5 / 110.5	1215	P86P (CCC→CCT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (33/31); new base A (597/549); total (631/584)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1217 total aligned reads displayed.

CGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTG  >  NC_002947/5984714‑5985009

                   ccccAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATCATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGgc                                                                                                                                   <  8:334804/146‑1 (MQ=255)

                                                       aTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCg                                                                                              >  3:353359/1‑149 (MQ=255)

                                                                           tgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGcc                                                                                 <  5:101611/142‑1 (MQ=255)

                                                                                  ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGa                                                                         >  7:165400/1‑143 (MQ=255)

                                                                                                    cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                   >  3:132210/8‑147 (MQ=11)

                                                                                                    cttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                   >  4:141737/8‑147 (MQ=11)

                                                                                                     ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                >  3:240252/7‑149 (MQ=11)

                                                                                                     ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                >  3:308946/7‑149 (MQ=11)

                                                                                                     ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                           <  8:177333/116‑1 (MQ=11)

                                                                                                      tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                               >  4:31830/6‑149 (MQ=11)

                                                                                                       caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                <  2:37937/143‑1 (MQ=11)

                                                                                                       caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                <  7:298864/143‑1 (MQ=11)

                                                                                                       caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              >  3:92674/5‑149 (MQ=11)

                                                                                                        accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              <  1:360159/145‑1 (MQ=11)

                                                                                                        accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATATCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              >  5:142249/4‑148 (MQ=11)

                                                                                                         ccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCAGCAGGc                                              >  1:279357/3‑148 (MQ=11)

                                                                                                         ccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                             >  1:166723/3‑148 (MQ=255)

                                                                                                          cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  1:148589/2‑148 (MQ=255)

                                                                                                           gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaag                                          >  3:182832/1‑148 (MQ=255)

                                                                                                           gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                          <  3:79184/149‑1 (MQ=255)

                                                                                                           gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGCCGGGC‑AATGAACGGCGCATACGGGAACATCTCTATCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAACCTGGTGGTGAGGATGGTCGGCACGCCa                                            >  5:185832/1‑147 (MQ=14)

                                                                                                            ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGc                                                                             <  4:256294/113‑1 (MQ=14)

                                                                                                            ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  6:238317/1‑146 (MQ=255)

                                                                                                             cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                <  2:229302/109‑1 (MQ=255)

                                                                                                             cTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                                                       <  2:126622/86‑1 (MQ=11)

                                                                                                             cTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTG                                                                 >  3:146433‑M1/1‑124 (MQ=255)

                                                                                                             cTTGACGAA‑TCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                       >  3:279266/1‑149 (MQ=255)

                                                                                                               tGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      >  2:149366/1‑149 (MQ=255)

                                                                                                                gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  7:318466/148‑1 (MQ=255)

                                                                                                                gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  3:214358/148‑1 (MQ=255)

                                                                                                                gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACt                                     >  6:344333/1‑149 (MQ=255)

                                                                                                                gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCAGCAGGCCAAAGAAc                                      <  8:7101/148‑1 (MQ=14)

                                                                                                                gACGAAATCCTCGTTGTCCCAGGCGTGGTTCTGGCCAGGGCGGGC‑AATGTACGGCTCATCCGGGAACATCTCTTTCAGCTCGGTCACCAGCGGGCCTTTCGGGCCTGGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAATGaa                                       <  4:91013/147‑1 (MQ=11)

                                                                                                                caCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  1:310000/147‑1 (MQ=255)

                                                                                                                 aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATGTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    >  7:209252/1‑149 (MQ=14)

                                                                                                                 aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    >  7:98579/1‑149 (MQ=255)

                                                                                                                 aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  3:328674/149‑1 (MQ=255)

                                                                                                                 aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  1:246474/149‑1 (MQ=255)

                                                                                                                 aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTG                                                                 <  2:345112‑M1/120‑1 (MQ=255)

                                                                                                                  cGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  1:53321/149‑1 (MQ=255)

                                                                                                                  cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  5:204209/148‑1 (MQ=255)

                                                                                                                  cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  7:63276/149‑1 (MQ=255)

                                                                                                                  cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  1:10148/149‑1 (MQ=255)

                                                                                                                  cGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  5:8943/148‑1 (MQ=14)

                                                                                                                  cGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  3:296328/149‑1 (MQ=14)

                                                                                                                   gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCTCCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              <  6:273559/137‑1 (MQ=11)

                                                                                                                   gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  >  8:145533/1‑149 (MQ=255)

                                                                                                                     actcctCGCTGTCCCAGGCGGTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCCGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGcc                                <  8:239682/144‑1 (MQ=14)

                                                                                                                     aatcctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              >  4:331272/6‑135 (MQ=255)

                                                                                                                        cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCg                                                    >  4:160719/3‑126 (MQ=255)

                                                                                                                         ctcGTTGTCCCAGGCGTTGATCTGGTCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            >  5:65834‑M1/2‑149 (MQ=255)

                                                                                                                         ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            <  6:212250‑M1/148‑1 (MQ=255)

                                                                                                                         ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            >  8:220376‑M1/2‑149 (MQ=255)

                                                                                                                         ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGATCGGCAGGCCAAAGAACTTGGCCAGGT                            <  8:201292‑M1/148‑1 (MQ=255)

                                                                                                                         ctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                             <  3:105599/147‑1 (MQ=255)

                                                                                                                          tCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                       <  7:259699/121‑1 (MQ=255)

                                                                                                                          tCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTC                           >  6:323432‑M1/1‑149 (MQ=255)

                                                                                                                             ttGTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCC                        <  5:264035‑M1/149‑1 (MQ=255)

                                                                                                                               gTCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                        >  6:65746/1‑99 (MQ=255)

                                                                                                                                tCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   >  6:16161/1‑135 (MQ=255)

                                                                                                                                tCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAA                     <  8:120585‑M1/149‑1 (MQ=255)

                                                                                                                                tCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCC                       >  7:116947‑M1/1‑147 (MQ=255)

                                                                                                                                tCCCAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCC                       >  8:4957‑M1/1‑147 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  8:262627/1‑149 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  1:78330/1‑149 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  4:291783/1‑149 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  7:78905/1‑149 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    <  3:161819/148‑1 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    <  6:91316/148‑1 (MQ=255)

                                                                                                                                  ccAGGCGTTGATCTGGCCAGCGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATACGGGCCTTGCTCGAAGCTGGTGCTGAGGATGGTCGCCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  7:138845/1‑149 (MQ=17)

                                                                                                                                   cAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  >  7:186418/1‑149 (MQ=255)

                                                                                                                                   cAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  <  5:238317/149‑1 (MQ=255)

                                                                                                                                   cAGGCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  >  2:61687/1‑149 (MQ=255)

                                                                                                                                      gCGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   >  1:210520/1‑129 (MQ=255)

                                                                                                                                       cGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc              <  2:301006/149‑1 (MQ=255)

                                                                                                                                       cGTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc              <  4:78407/149‑1 (MQ=255)

                                                                                                                                        gTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  6:294239/1‑149 (MQ=255)

                                                                                                                                        gTTGATCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  1:182885/1‑149 (MQ=255)

                                                                                                                                         ttGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                                                         >  3:258272/1‑56 (MQ=255)

                                                                                                                                         atGATCTCGCCAGGGCGGGC‑AATCTACGGCGCAACCGGGAACATCTCTTTCAGCTCGGGCACCAGCTGGCCATTCGGGCCTTGCTCGAAGCTGGTGCTGAGCATGGTCGGCAGGCCAAAGAACATGGCCAGGTCGCCCAACGCCAGAAc            >  4:4239/2‑149 (MQ=17)

                                                                                                                                           GATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACg           >  6:136332‑M1/1‑148 (MQ=255)

                                                                                                                                            ATCTGGCCAGGTCTGCC‑AATGGACGGCGCATCCGTCAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGtt         >  4:190432‑M1/1‑149 (MQ=255)

                                                                                                                                            ATCTGACCAGGGCGGCC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGAACAAGCGGGCCATTCGGGCCTAGCTCTAAGCTGGTGGTGAGGATGGACGGCAGGCCAAAGAACTTGGCCAGGTAGCCAAAGGCCAGCACGtt         <  2:165481‑M1/149‑1 (MQ=255)

                                                                                                                                             tCTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        >  6:38152/1‑149 (MQ=255)

                                                                                                                                              cTGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  2:275966/148‑1 (MQ=255)

                                                                                                                                               tGGCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgtt      >  5:287715/1‑149 (MQ=255)

                                                                                                                                                ggCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    >  5:354236/1‑134 (MQ=255)

                                                                                                                                                ggCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc     >  6:11228/1‑149 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTAGGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACAAGCGGGCCATTCGGGCCTTGCTCGAAGCTGTTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCATGTCGCCCAAGGCCAGCACgtt         <  7:335518/144‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACCTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTGGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgt       <  6:191268/146‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                          <  4:235760/95‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  5:47453/115‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgtt      <  1:330795/147‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  5:326084/145‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  4:110841/145‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  8:302252/145‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgtt         <  2:202404/144‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc     <  5:157216/148‑1 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt    >  7:23839/1‑149 (MQ=255)

                                                                                                                                                 gCCAGGGCGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt    >  2:110537/1‑149 (MQ=255)

CGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGC‑AATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTG  >  NC_002947/5984714‑5985009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`