BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,984,865	A→G	94.8%	R85R (CGT→CGC)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,984,865	0	A	G	94.8%	3399.8 / 105.7	1215	R85R (CGT→CGC)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (32/31); new base G (596/552); total (631/584)
Fisher's exact test for biased strand distribution p-value = 8.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

Only 100 of 1215 total aligned reads displayed.

CACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTG  >  NC_002947/5984718‑5985009

               ccccAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATCATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGgc                                                                                                                                    <  8:334804/146‑1 (MQ=255)

                                                   aTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCg                                                                                              >  3:353359/1‑149 (MQ=255)

                                                                              ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGa                                                                         >  7:165400/1‑143 (MQ=255)

                                                                                                 ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                >  3:240252/7‑149 (MQ=11)

                                                                                                 ttcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                           <  8:177333/116‑1 (MQ=11)

                                                                                                   caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                <  2:37937/143‑1 (MQ=11)

                                                                                                   caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                <  7:298864/143‑1 (MQ=11)

                                                                                                   caccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                <  6:367732/143‑1 (MQ=11)

                                                                                                    accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              <  1:360159/145‑1 (MQ=11)

                                                                                                    accgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATATCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              >  5:142249/4‑148 (MQ=11)

                                                                                                     ccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                             >  1:166723/3‑148 (MQ=255)

                                                                                                      cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCgg                                                                                    <  6:343620/107‑1 (MQ=11)

                                                                                                      cgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  1:148589/2‑148 (MQ=255)

                                                                                                       gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaag                                          >  3:182832/1‑148 (MQ=255)

                                                                                                        ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  6:238317/1‑146 (MQ=255)

                                                                                                        ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  4:255328/1‑146 (MQ=255)

                                                                                                        ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCa                                            >  3:277680/1‑146 (MQ=255)

                                                                                                        ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGT                                                                  >  4:140843‑M1/1‑124 (MQ=255)

                                                                                                         cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                <  2:229302/109‑1 (MQ=255)

                                                                                                          ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGa                                        >  7:262296/1‑148 (MQ=255)

                                                                                                           tGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      >  2:149366/1‑149 (MQ=255)

                                                                                                            gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                            <  8:240503/110‑1 (MQ=14)

                                                                                                            gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  7:318466/148‑1 (MQ=255)

                                                                                                            gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  3:214358/148‑1 (MQ=255)

                                                                                                            gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCAGCAGGCCAAAGAAc                                      <  8:7101/148‑1 (MQ=14)

                                                                                                            caCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  1:310000/147‑1 (MQ=255)

                                                                                                             aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  1:246474/149‑1 (MQ=255)

                                                                                                             aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  3:328674/149‑1 (MQ=255)

                                                                                                             aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTG                                                                 <  2:345112‑M1/120‑1 (MQ=255)

                                                                                                              cGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  1:53321/149‑1 (MQ=255)

                                                                                                              cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  5:204209/148‑1 (MQ=255)

                                                                                                              cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  1:10148/149‑1 (MQ=255)

                                                                                                              cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  7:63276/149‑1 (MQ=255)

                                                                                                              cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   <  6:322750/149‑1 (MQ=255)

                                                                                                              cGAAATCCTCGTTGTCCCAGGCGGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                    <  5:8943/148‑1 (MQ=14)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCTCCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGc                                              <  6:273559/137‑1 (MQ=11)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                               <  7:80406/136‑1 (MQ=255)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  >  3:78407/1‑149 (MQ=255)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  >  8:145533/1‑149 (MQ=255)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  >  2:126501/1‑149 (MQ=255)

                                                                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  <  6:158224/149‑1 (MQ=255)

                                                                                                               gAAATCCTCGTTGTCACAGGCGTTGATGTGGCCAGGGCGGGCATTGTACGGCGCATCCG‑CGAACATCACTTTCCGCTCGGGCAACAGCGGGCCATTCGGGCCTTGCTCGAAGGTGGAGGTGAGGATGGTCGGCAGGCCAAAGAACTTgg                                  >  6:190252/1‑149 (MQ=255)

                                                                                                                 aatcctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAgg                                                           >  4:228059/6‑122 (MQ=255)

                                                                                                                   tcctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                              <  7:118009/146‑1 (MQ=255)

                                                                                                                    cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGATGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGACGATGGTCGGCAGGCAAAAGAACTTGGCCAg                              >  1:279100/3‑149 (MQ=14)

                                                                                                                    cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCg                                                    <  3:160719/124‑1 (MQ=255)

                                                                                                                    cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCg                                                    >  4:160719/3‑126 (MQ=255)

                                                                                                                     ctcGTTGTCCCAGGCGTTGATCTGGTCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            >  5:65834‑M1/2‑149 (MQ=255)

                                                                                                                     ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            <  6:212250‑M1/148‑1 (MQ=255)

                                                                                                                     ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGT                            >  8:220376‑M1/2‑149 (MQ=255)

                                                                                                                     ctcGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGATCGGCAGGCCAAAGAACTTGGCCAGGT                            <  8:201292‑M1/148‑1 (MQ=255)

                                                                                                                     ctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                             <  3:105599/147‑1 (MQ=255)

                                                                                                                        gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGC                         <  3:14445‑M1/149‑1 (MQ=255)

                                                                                                                        gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGC                         >  7:209840‑M1/1‑149 (MQ=255)

                                                                                                                         ttGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCC                        <  3:91859‑M1/149‑1 (MQ=255)

                                                                                                                         ttGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCC                        <  5:264035‑M1/149‑1 (MQ=255)

                                                                                                                           gTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                        >  6:65746/1‑99 (MQ=255)

                                                                                                                            tCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAA                     <  8:120585‑M1/149‑1 (MQ=255)

                                                                                                                            tCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCC                       >  8:4957‑M1/1‑147 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  2:300140/1‑149 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCATCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  3:127327/1‑149 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  1:78330/1‑149 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  8:262627/1‑149 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    <  4:318002/148‑1 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    <  6:91316/148‑1 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                    <  4:98275/148‑1 (MQ=255)

                                                                                                                              ccAGGCGTTGATCTGGCCAGCGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATACGGGCCTTGCTCGAAGCTGGTGCTGAGGATGGTCGCCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                   >  7:138845/1‑149 (MQ=17)

                                                                                                                               cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  >  2:61687/1‑149 (MQ=255)

                                                                                                                               cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  >  7:186418/1‑149 (MQ=255)

                                                                                                                               cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  <  5:238317/149‑1 (MQ=255)

                                                                                                                               cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  <  3:294283/149‑1 (MQ=255)

                                                                                                                               cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  <  4:287412/149‑1 (MQ=255)

                                                                                                                                aGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                 <  5:353672/149‑1 (MQ=255)

                                                                                                                                  gCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                   >  1:210520/1‑129 (MQ=255)

                                                                                                                                  gCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                  >  4:90917/1‑146 (MQ=255)

                                                                                                                                   cGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc              <  5:183901/149‑1 (MQ=255)

                                                                                                                                    gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  6:294239/1‑149 (MQ=255)

                                                                                                                                    gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  1:182885/1‑149 (MQ=255)

                                                                                                                                    gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  3:257087/1‑149 (MQ=255)

                                                                                                                                    gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCa             >  8:10213/1‑149 (MQ=255)

                                                                                                                                     atGATCTCGCCAGGGCGGGCAATCTACGGCGCAACCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCTGGCCATTCGGGCCTTGCTCGAAGCTGGTGCTGAGCATGGTCGGCAGGCCAAAGAACATGGCCAGGTCGCCCAACGCCAGAAc            >  4:4239/2‑149 (MQ=17)

                                                                                                                                      TGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACg           <  4:330675‑M1/149‑1 (MQ=255)

                                                                                                                                       GATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACg           >  6:136332‑M1/1‑148 (MQ=255)

                                                                                                                                        ATCTGGCCAGGTCTGCCAATGGACGGCGCATCCG‑TCAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGtt         >  4:190432‑M1/1‑149 (MQ=255)

                                                                                                                                        ATCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGtt         <  7:238555‑M1/149‑1 (MQ=255)

                                                                                                                                        ATCTGGCCAGGGCGGGCAATGTACGGCGCATACG‑GCAACATCTCAATCAGCTCGGGCACCACCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGCCCAGCACGtt         <  2:104199‑M1/149‑1 (MQ=255)

                                                                                                                                        ATCTGACCAGGGCGGCCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGAACAAGCGGGCCATTCGGGCCTAGCTCTAAGCTGGTGGTGAGGATGGACGGCAGGCCAAAGAACTTGGCCAGGTAGCCAAAGGCCAGCACGtt         <  2:165481‑M1/149‑1 (MQ=255)

                                                                                                                                         tCTGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        >  4:110694/1‑149 (MQ=255)

                                                                                                                                           tGGCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgtt      >  5:287715/1‑149 (MQ=255)

                                                                                                                                            ggCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc     >  6:11228/1‑149 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTAGGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACAAGCGGGCCATTCGGGCCTTGCTCGAAGCTGTTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCATGTCGCCCAAGGCCAGCACgtt         <  7:335518/144‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                      <  5:47453/115‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttgtt      <  1:330795/147‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  4:76588/145‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  5:326084/145‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg        <  8:302252/145‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgtt         <  2:202404/144‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc     <  5:157216/148‑1 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGAGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt    >  3:308335/1‑149 (MQ=255)

                                                                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCG‑GGAACATCTCTATCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCGCAAGGCCAGCAAGTTGTTCt    >  2:271394/1‑149 (MQ=18)

CACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCG‑GGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTG  >  NC_002947/5984718‑5985009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`