Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,087,353 G→T 7.5% R292L (CGG→CTG)  oruR → ornithine utilization transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,087,3530GT 7.5% 204.3 / 4.2 65R292L (CGG→CTG) oruRornithine utilization transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (26/34);  new base T (3/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCGCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCAT  >  NC_002947/6087205‑6087501
                                                                                                                                                    |                                                                                                                                                      
gagcgCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGTATTGATTCGCCATCTGCTCGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCg                                                                                                                                                        <  1:115298/149‑1 (MQ=255)
        ccGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt                                                                                                                                                  <  8:220286/147‑1 (MQ=255)
             gcCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGgcg                                                                                                                                           >  8:246785/1‑149 (MQ=255)
                  aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                      <  4:49639/149‑1 (MQ=255)
                  aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                      <  5:65126/149‑1 (MQ=255)
                  aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGgcgcag                                                                                                                                        <  2:274846/147‑3 (MQ=255)
                   aCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgc                                                                                                                                     <  2:215296/149‑1 (MQ=255)
                    ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                      >  2:74690/1‑147 (MQ=255)
                    ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgca                                                                                                                                    <  2:301031/149‑1 (MQ=255)
                     cTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCGCCCAGCGCCTGCAAATGCCGGCGCAgggtgc                                                                                                                                   >  5:104383/1‑146 (MQ=255)
                        gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                       >  4:20075/1‑142 (MQ=255)
                        gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                       <  3:20075/142‑1 (MQ=255)
                         aaTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGt                                                                                                                               <  6:101509/149‑1 (MQ=255)
                          aTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCTGCAAATGCCGGCGCAgg                                                                                                                                       <  7:72620/140‑1 (MQ=255)
                          aTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCTGCAAATGCCGGCGCAgg                                                                                                                                       >  8:72620/1‑140 (MQ=255)
                           ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                       >  5:301408/1‑139 (MQ=255)
                           ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                       <  6:301408/139‑1 (MQ=255)
                           ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGGCGCAgggtgcgcg                                                                                                                                >  6:182254/1‑140 (MQ=255)
                              aCCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGTCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca                                                                                                                                 <  6:238597/142‑1 (MQ=255)
                              aCCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca                                                                                                                                 >  5:238597/1‑142 (MQ=255)
                               ccGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCaa                                                                                                                         <  2:197918/149‑1 (MQ=255)
                                cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTAt                                                                                                                            >  8:127748/1‑145 (MQ=255)
                                cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTAt                                                                                                                            <  7:127748/145‑1 (MQ=255)
                                cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                        <  8:182077/149‑1 (MQ=255)
                                  gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTg                                                                                                                                                 >  5:249953/1‑122 (MQ=255)
                                  gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTg                                                                                                                                                 <  6:249953/122‑1 (MQ=255)
                                  gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAg                                                                                                                      >  8:33433/1‑149 (MQ=255)
                                     gCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                        <  2:253494/144‑1 (MQ=255)
                                     gCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                        >  1:253494/1‑144 (MQ=255)
                                           cATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGAc                                                                                                            <  8:6781/149‑1 (MQ=255)
                                             tGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                          <  7:33433/149‑1 (MQ=255)
                                             tGGCTGGGACGGATTCGCCAGCGGCTGGCGCAGCAACTTGACGCAGCACCCGGGCTTGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                          <  6:234641/149‑1 (MQ=255)
                                               gCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGc                                                                                                        >  1:17770/1‑149 (MQ=255)
                                                       ggATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGGTc                                                                                               >  1:211393/1‑149 (MQ=255)
                                                                        gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                          <  6:272318/122‑1 (MQ=255)
                                                                        gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                          >  5:272318/1‑122 (MQ=255)
                                                                        gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGtt                                                                              >  3:219257/1‑149 (MQ=255)
                                                                            agcaACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTg                                                                          <  3:172486/149‑1 (MQ=255)
                                                                              caACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGc                                                                        <  5:192310/149‑1 (MQ=255)
                                                                                    gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCCGTCGCTGGGCAGCAGTTATCAACACCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCTGTTGCTGGCCGAt                                                                    <  2:55419/147‑1 (MQ=255)
                                                                                    gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCt                                                                                                                    >  3:8410/1‑101 (MQ=255)
                                                                                    gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCt                                                                                                                    <  4:8410/101‑1 (MQ=255)
                                                                                    gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt                                                                    <  1:74690/148‑1 (MQ=255)
                                                                                    gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt                                                                    <  2:17770/147‑1 (MQ=255)
                                                                                       gcagcaCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGAGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa                                                                 >  4:259090/1‑147 (MQ=255)
                                                                                         agcaCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa                                                                 >  6:275569/1‑145 (MQ=255)
                                                                                            aCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGc                                                          <  4:289832/149‑1 (MQ=255)
                                                                                                gggCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGAt                                                      <  4:219257/149‑1 (MQ=255)
                                                                                                 ggCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATc                                                     >  7:152144/1‑149 (MQ=255)
                                                                                                          ggCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGAtt                                            <  8:152144/149‑1 (MQ=255)
                                                                                                                cgcgGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGCTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCaga                                       <  2:211393/148‑1 (MQ=255)
                                                                                                                      aGATGAACTGCTCTTCGTGCACCCTGCGACGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTg                                <  3:259090/149‑1 (MQ=255)
                                                                                                                      aGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTg                                <  7:246785/149‑1 (MQ=255)
                                                                                                                       gATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                               <  5:210276/149‑1 (MQ=255)
                                                                                                                           aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGACGGTTCGAGCGGGCCAAGCAGTTGCTg                                                                          <  1:4702/103‑1 (MQ=255)
                                                                                                                           aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTg                                                                          >  2:4702/1‑102 (MQ=255)
                                                                                                                           aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGtt                           >  5:327654/1‑149 (MQ=255)
                                                                                                                              tGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGACCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                        >  2:316343/1‑149 (MQ=255)
                                                                                                                                  ctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                               >  2:41984/1‑138 (MQ=255)
                                                                                                                                  ctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                               <  1:41984/138‑1 (MQ=255)
                                                                                                                                   ttCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGAGTTTGGGGTTCAGTgaga                   >  8:341433/1‑149 (MQ=255)
                                                                                                                                            ccTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGtt          >  4:205963/1‑149 (MQ=255)
                                                                                                                                             cTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                        >  6:88471/1‑134 (MQ=255)
                                                                                                                                             cTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                        <  5:88471/134‑1 (MQ=255)
                                                                                                                                                 gcCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCgg     >  8:2919/1‑149 (MQ=255)
                                                                                                                                                   cGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCa   >  5:220583/1‑149 (MQ=255)
                                                                                                                                                    ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt                       >  6:223034/1‑128 (MQ=255)
                                                                                                                                                    ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt                       <  5:223034/128‑1 (MQ=255)
                                                                                                                                                    ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCAt  <  1:163185/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GAGCGCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCAT  >  NC_002947/6087205‑6087501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑