Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,087,358	T→A	6.9%	L294M (TTG→ATG)	oruR →	ornithine utilization transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,087,358	0	T	A	6.9%	202.6 / 3.4	68	L294M (TTG→ATG)	oruR	ornithine utilization transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (28/35);  new base A (3/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCATGCC  >  NC_002947/6087213‑6087504                                                                                                                                                  |                                                                                                                                                     ccGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt                                                                                                                                                     <  8:220286/147‑1 (MQ=255)      gcCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGgcg                                                                                                                                              >  8:246785/1‑149 (MQ=255)           aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                         <  4:49639/149‑1 (MQ=255)           aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                         <  5:65126/149‑1 (MQ=255)           aaCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGgcgcag                                                                                                                                           <  2:274846/147‑3 (MQ=255)            aCCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgc                                                                                                                                        <  2:215296/149‑1 (MQ=255)             ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg                                                                                                                                         >  2:74690/1‑147 (MQ=255)             ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgca                                                                                                                                       <  2:301031/149‑1 (MQ=255)              cTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCGCCCAGCGCCTGCAAATGCCGGCGCAgggtgc                                                                                                                                      >  5:104383/1‑146 (MQ=255)                 gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                          >  4:20075/1‑142 (MQ=255)                 gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                          <  3:20075/142‑1 (MQ=255)                  aaTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGt                                                                                                                                  <  6:101509/149‑1 (MQ=255)                   aTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCTGCAAATGCCGGCGCAgg                                                                                                                                          >  8:72620/1‑140 (MQ=255)                   aTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCTGCAAATGCCGGCGCAgg                                                                                                                                          <  7:72620/140‑1 (MQ=255)                    ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                          <  6:301408/139‑1 (MQ=255)                    ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg                                                                                                                                          >  5:301408/1‑139 (MQ=255)                    ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGGCGCAgggtgcgcg                                                                                                                                   >  6:182254/1‑140 (MQ=255)                       aCCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGTCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca                                                                                                                                    <  6:238597/142‑1 (MQ=255)                       aCCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca                                                                                                                                    >  5:238597/1‑142 (MQ=255)                        ccGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCaa                                                                                                                            <  2:197918/149‑1 (MQ=255)                         cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTAt                                                                                                                               <  7:127748/145‑1 (MQ=255)                         cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTAt                                                                                                                               >  8:127748/1‑145 (MQ=255)                         cGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                           <  8:182077/149‑1 (MQ=255)                           gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTg                                                                                                                                                    <  6:249953/122‑1 (MQ=255)                           gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTg                                                                                                                                                    >  5:249953/1‑122 (MQ=255)                           gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAg                                                                                                                         >  8:33433/1‑149 (MQ=255)                              gCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                           <  2:253494/144‑1 (MQ=255)                              gCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAAc                                                                                                                           >  1:253494/1‑144 (MQ=255)                                    cATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGAc                                                                                                               <  8:6781/149‑1 (MQ=255)                                      tGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                             <  7:33433/149‑1 (MQ=255)                                      tGGCTGGGACGGATTCGCCAGCGGCTGGCGCAGCAACTTGACGCAGCACCCGGGCTTGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                             <  6:234641/149‑1 (MQ=255)                                        gCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGc                                                                                                           >  1:17770/1‑149 (MQ=255)                                                ggATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGGTc                                                                                                  >  1:211393/1‑149 (MQ=255)                                                                 gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                             <  6:272318/122‑1 (MQ=255)                                                                 gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGa                                                                                                             >  5:272318/1‑122 (MQ=255)                                                                 gcgcAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGtt                                                                                 >  3:219257/1‑149 (MQ=255)                                                                     agcaACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTg                                                                             <  3:172486/149‑1 (MQ=255)                                                                       caACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGc                                                                           <  5:192310/149‑1 (MQ=255)                                                                             gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCCGTCGCTGGGCAGCAGTTATCAACACCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCTGTTGCTGGCCGAt                                                                       <  2:55419/147‑1 (MQ=255)                                                                             gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCt                                                                                                                       >  3:8410/1‑101 (MQ=255)                                                                             gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCt                                                                                                                       <  4:8410/101‑1 (MQ=255)                                                                             gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt                                                                       <  1:74690/148‑1 (MQ=255)                                                                             gACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt                                                                       <  2:17770/147‑1 (MQ=255)                                                                                gcagcaCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGAGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa                                                                    >  4:259090/1‑147 (MQ=255)                                                                                  agcaCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa                                                                    >  6:275569/1‑145 (MQ=255)                                                                                     aCCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGc                                                             <  4:289832/149‑1 (MQ=255)                                                                                         gggCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGAt                                                         <  4:219257/149‑1 (MQ=255)                                                                                          ggCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATc                                                        >  7:152144/1‑149 (MQ=255)                                                                                                   ggCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGAtt                                               <  8:152144/149‑1 (MQ=255)                                                                                                         cgcgGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGCTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCaga                                          <  2:211393/148‑1 (MQ=255)                                                                                                               aGATGAACTGCTCTTCGTGCACCCTGCGACGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTg                                   <  3:259090/149‑1 (MQ=255)                                                                                                               aGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTg                                   <  7:246785/149‑1 (MQ=255)                                                                                                                gATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                                  <  5:210276/149‑1 (MQ=255)                                                                                                                    aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGACGGTTCGAGCGGGCCAAGCAGTTGCTg                                                                             <  1:4702/103‑1 (MQ=255)                                                                                                                    aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTg                                                                             >  2:4702/1‑102 (MQ=255)                                                                                                                    aaCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGtt                              >  5:327654/1‑149 (MQ=255)                                                                                                                       tGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGACCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                           >  2:316343/1‑149 (MQ=255)                                                                                                                           ctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                                  >  2:41984/1‑138 (MQ=255)                                                                                                                           ctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg                                  <  1:41984/138‑1 (MQ=255)                                                                                                                            ttCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGAGTTTGGGGTTCAGTgaga                      >  8:341433/1‑149 (MQ=255)                                                                                                                                     ccTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGtt             >  4:205963/1‑149 (MQ=255)                                                                                                                                      cTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                           >  6:88471/1‑134 (MQ=255)                                                                                                                                      cTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg                           <  5:88471/134‑1 (MQ=255)                                                                                                                                          gcCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCgg        >  8:2919/1‑149 (MQ=255)                                                                                                                                            cGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCa      >  5:220583/1‑149 (MQ=255)                                                                                                                                             ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt                          >  6:223034/1‑128 (MQ=255)                                                                                                                                             ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt                          <  5:223034/128‑1 (MQ=255)                                                                                                                                             ggCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCAt     <  1:163185/149‑1 (MQ=255)                                                                                                                                                aTTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCATGcc  >  5:269833/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     CCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAGCACCCGGGCTGGAAGGGCTTGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTG‑TTGGACGAGCTGA‑GGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGCATGCC  >  NC_002947/6087213‑6087504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑