Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,127,164 C→G 6.6% V241L (GTG→CTG) ‡ PP_5375 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,127,1640CG 6.6% 235.3 / 4.4 75V241L (GTG→CTG) ‡PP_5375LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base G (3/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

CCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGC  >  NC_002947/6127021‑6127303
                                                                                                                                               |                                                                                                                                           
ccGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCt                                                                                                                                        >  7:126680/1‑149 (MQ=255)
  gCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTgg                                                                                                                                      >  4:6915/1‑149 (MQ=255)
    aCGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGcc                                                                                                                                    <  4:327956/149‑1 (MQ=255)
      gCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCAc                                                                                                                                  >  4:128176/1‑149 (MQ=255)
        tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACt                                                                                                                                 >  3:192586/1‑148 (MQ=255)
        tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACt                                                                                                                                 >  3:279682/1‑148 (MQ=255)
            aCGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCACGTTGCCCCAGGCCAGGGCCAAGCCCTGGCCACTGATct                                                                                                                            >  7:340485/1‑148 (MQ=255)
              gAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGcc                                                                                                                          >  7:132403/1‑149 (MQ=255)
                 gACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAc                                                                                                                       >  6:238193/1‑149 (MQ=255)
                 gACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAc                                                                                                                       >  7:30423/1‑149 (MQ=255)
                 gACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAc                                                                                                                       >  7:24979/1‑149 (MQ=255)
                  aCCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACt                                                                                                                      <  3:128176/149‑1 (MQ=255)
                   ccACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGAt                                                                                                                              <  8:324867/140‑1 (MQ=255)
                   ccACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGAt                                                                                                                              >  7:324867/1‑140 (MQ=255)
                   ccACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTg                                                                                                                     <  8:132403/149‑1 (MQ=255)
                    cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTg                                                                                                                     >  1:165369/1‑148 (MQ=255)
                    cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTg                                                                                                                     >  3:211149/1‑148 (MQ=255)
                       gCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATc                                                                                                                 <  6:37224/149‑1 (MQ=255)
                        cTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCa                                                                                                                <  8:24979/149‑1 (MQ=255)
                        cTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCa                                                                                                                <  8:30423/149‑1 (MQ=255)
                        cTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCa                                                                                                                <  4:192586/149‑1 (MQ=255)
                          cGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCGCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGc                                                                                                              <  8:126680/149‑1 (MQ=255)
                             tGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATa                                                                                                           <  2:92091/149‑1 (MQ=255)
                                  gacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTa                                                                                                      <  6:23720/149‑1 (MQ=255)
                                   acTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAg                                                                                                     <  5:238193/149‑1 (MQ=255)
                                    cTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCa                                                                                                                                   >  6:263664/1‑118 (MQ=255)
                                    cTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCa                                                                                                                                   <  5:263664/118‑1 (MQ=255)
                                     tCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAc                                                                                                                       <  7:161116/129‑1 (MQ=255)
                                     tCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAc                                                                                                                       >  8:161116/1‑129 (MQ=255)
                                     tCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCt                                                                                                   >  4:323554/1‑149 (MQ=255)
                                       cagcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGt                                                                                                 <  3:279922/149‑1 (MQ=255)
                                         gcaggcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGtt                                                                                                >  5:177788/1‑148 (MQ=255)
                                          caggcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGAt                                                                                                                  >  4:310745/1‑129 (MQ=255)
                                          caggcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGAt                                                                                                                  <  3:310745/129‑1 (MQ=255)
                                             gcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGatat                                                                                           >  3:269218/1‑149 (MQ=255)
                                               agAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCGCTGGccgggg                                                                                                                                >  5:192125/1‑106 (MQ=255)
                                               agAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctggg                                                                                                                                <  6:192125/110‑5 (MQ=255)
                                                gAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGa                                                                                        >  7:87171/1‑149 (MQ=255)
                                                  aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcg                                                                                      <  8:87171/149‑1 (MQ=255)
                                                      ggCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGGACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGa                                                                                   <  1:118974/148‑1 (MQ=255)
                                                         gTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGc                                                                                >  7:212194/1‑148 (MQ=255)
                                                         gTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGc                                                                                <  8:212194/148‑1 (MQ=255)
                                                              tCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACACATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg                                                                            <  3:73571/147‑1 (MQ=255)
                                                                     tACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATgg                                                                   <  4:269218/149‑1 (MQ=255)
                                                                         acaTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc                                                               <  2:165369/149‑1 (MQ=255)
                                                                         acaTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc                                                               <  3:6915/149‑1 (MQ=255)
                                                                           aTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCg                                                              >  5:349448/1‑148 (MQ=255)
                                                                                       gACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCt                                                 >  5:329937/1‑149 (MQ=255)
                                                                                            gcgcACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAAc                                            >  4:47500/1‑149 (MQ=255)
                                                                                                   ggTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                     <  3:323554/149‑1 (MQ=255)
                                                                                                       cTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                  <  1:44644/132‑1 (MQ=255)
                                                                                                       cTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                  >  2:44644/1‑132 (MQ=255)
                                                                                                       cTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCTGGcc                                  >  5:49870/1‑148 (MQ=255)
                                                                                                          aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCAt                                                                     >  6:90890/1‑110 (MQ=255)
                                                                                                          aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCAt                                                                     <  5:90890/110‑1 (MQ=255)
                                                                                                                tCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCa                        <  6:177788/149‑1 (MQ=255)
                                                                                                                 cGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAg                       >  5:66809/1‑149 (MQ=255)
                                                                                                                  gTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGAGTGGATAGGCATACCGAAGCGGTTGATATTGAGCCGATCGTTGGGGGGCAGGGGGTCGTATCCACGGCGGTTCAACCATAGGGGCCAACCACCCCAgg                      >  4:186452/1‑148 (MQ=255)
                                                                                                                   tCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTAc                           >  6:41950/1‑143 (MQ=255)
                                                                                                                   tCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTAc                           <  5:41950/143‑1 (MQ=255)
                                                                                                                      accaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTcgcg                  >  2:222568/1‑149 (MQ=255)
                                                                                                                       ccaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCgcgc                 <  4:279682/149‑1 (MQ=255)
                                                                                                                         aGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTg               >  4:325319/1‑149 (MQ=255)
                                                                                                                          ggTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTgg              <  3:47500/149‑1 (MQ=255)
                                                                                                                                cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCt        >  7:183326/1‑149 (MQ=255)
                                                                                                                                   tggccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTc      >  4:176795/2‑148 (MQ=255)
                                                                                                                                   aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTc      >  2:88542/1‑148 (MQ=255)
                                                                                                                                   aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCa     <  3:325319/149‑1 (MQ=255)
                                                                                                                                     gccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTAGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAag   >  5:242209/1‑149 (MQ=255)
                                                                                                                                      ccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa            <  2:132594/139‑1 (MQ=255)
                                                                                                                                      ccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa            >  1:132594/1‑139 (MQ=255)
                                                                                                                                      ccaggGCCACGCCCTGGCCACTGATCGCCGACTGTATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagc  <  6:49870/149‑1 (MQ=255)
                                                                                                                                      ccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagc  <  6:66809/149‑1 (MQ=255)
                                                                                                                                        aggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa            >  8:121458/1‑137 (MQ=255)
                                                                                                                                        aggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa            <  7:121458/137‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGC  >  NC_002947/6127021‑6127303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑