Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,142,207 A→T 8.6% E648V (GAA→GTA) ‡ cusA → CzcA family RND family copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,142,2070AT 8.6% 171.0 / 5.4 58E648V (GAA→GTA) ‡cusACzcA family RND family copper transporter
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base T (3/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCGGAGGTCGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACCACAC  >  NC_002947/6142062‑6142351
                                                                                                                                                 |                                                                                                                                                
ccGGAGGTCGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAAc                                                                                                                                                <  5:359163/148‑1 (MQ=255)
    aGGTCGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGac                                                                                                                                           <  6:35885/149‑1 (MQ=255)
    aGGTCGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTAGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGac                                                                                                                                           <  5:83489/149‑1 (MQ=255)
        cGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGaccgta                                                                                                                                       >  8:30767/1‑149 (MQ=255)
        cGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGaccgt                                                                                                                                        >  8:25359/1‑148 (MQ=255)
          ccAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACCGGTCGAGTTCCTCGaccagttt                                                                                                                                     <  3:120560/149‑6 (MQ=255)
              tgtgtTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACcgtaccgtac                                                                                                                                 <  8:48484/149‑1 (MQ=255)
                    cGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACCGGTCGAGTTCCTCGaccagt                                                                                                                                       <  8:313666/137‑4 (MQ=255)
                    cGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACCGGTCGAGTTCCTCGaccagt                                                                                                                                       >  7:313666/1‑134 (MQ=255)
                        aaaGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGCGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCggg                                                                                                                       >  5:324621/1‑149 (MQ=255)
                            ccGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTg                                                                                                                   >  7:284895/1‑149 (MQ=255)
                                  gcgcAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAAc                                                                                                             <  1:270541/149‑1 (MQ=255)
                                    gcAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACAt                                                                                                           >  4:199449/1‑149 (MQ=255)
                                     cAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATc                                                                                                          <  5:63903/149‑1 (MQ=255)
                                      agagTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCt                                                                                                         <  7:25359/149‑1 (MQ=255)
                                      agagTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCt                                                                                                         <  7:30767/149‑1 (MQ=255)
                                            aGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTc                                                                                                   >  5:305615/1‑149 (MQ=255)
                                                 ccGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTccgc                                                                                                <  8:284895/147‑1 (MQ=255)
                                                     cccGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATcc                                                                                          <  2:285914/149‑1 (MQ=255)
                                                           aCCGTTGGAGATGTTCGCGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAAcc                                                                                    <  8:334073/149‑1 (MQ=255)
                                                                   agaTGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAAc                                                                                                             <  3:345235/116‑1 (MQ=255)
                                                                   agaTGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAAc                                                                                                             >  4:345235/1‑116 (MQ=255)
                                                                    gaTGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACa                                                                           <  3:351266/149‑1 (MQ=255)
                                                                        ttCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGTGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTa                                                                                 >  8:255203/1‑139 (MQ=255)
                                                                        ttCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGTGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTa                                                                                 <  7:255203/139‑1 (MQ=255)
                                                                                cATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTg                                                               <  3:199449/149‑1 (MQ=255)
                                                                                    gtgCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGaa                                                            <  6:305615/148‑1 (MQ=255)
                                                                                         gCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGTGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAaga                                                      <  6:324621/149‑1 (MQ=255)
                                                                                         gCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATcc                                                                                          <  5:171618/113‑1 (MQ=255)
                                                                                         gCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATcc                                                                                          >  6:171618/1‑113 (MQ=255)
                                                                                            cAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGcc                                                   >  3:281863/1‑149 (MQ=255)
                                                                                                  cAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTg                                             >  4:311785/1‑149 (MQ=255)
                                                                                                     ggATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCg                                          >  1:324748/1‑149 (MQ=255)
                                                                                                           gTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAgg                                    >  8:217141/1‑149 (MQ=255)
                                                                                                           gTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAgg                                    >  6:234984/1‑149 (MQ=255)
                                                                                                           gTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAgg                                    <  5:234984/149‑1 (MQ=255)
                                                                                                           gTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTCGCGTAAAgg                                    >  2:284836/1‑149 (MQ=255)
                                                                                                                ggcCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGcc                               <  6:95660/149‑1 (MQ=255)
                                                                                                                 gcCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCg                              >  3:245129/1‑149 (MQ=255)
                                                                                                                  ccTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGc                                                                    <  2:319543/110‑1 (MQ=255)
                                                                                                                  ccTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGc                                                                    >  1:319543/1‑110 (MQ=255)
                                                                                                                      gAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGc                         >  2:257238/1‑149 (MQ=255)
                                                                                                                              agcGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTgg                  <  2:324748/148‑1 (MQ=255)
                                                                                                                                cGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGgcgc               <  4:281863/149‑1 (MQ=255)
                                                                                                                                 gagaAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGCCCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGgcg                >  8:198447/1‑147 (MQ=255)
                                                                                                                                     aaCTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATc          <  4:245129/149‑1 (MQ=255)
                                                                                                                                         ggTCGAGTTCCTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGAc       >  1:330731/1‑148 (MQ=255)
                                                                                                                                         ggTCGAGTTCCTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGAc       >  4:129125/1‑148 (MQ=255)
                                                                                                                                         ggTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGgc                 <  1:342853/138‑1 (MQ=255)
                                                                                                                                         ggTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGgc                 >  2:342853/1‑138 (MQ=255)
                                                                                                                                          gTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCaa                                                        >  8:175215/1‑98 (MQ=255)
                                                                                                                                          gTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCaa                                                        <  7:175215/98‑1 (MQ=255)
                                                                                                                                          gTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACca     >  5:107575/1‑149 (MQ=255)
                                                                                                                                          gTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACca     <  3:311785/149‑1 (MQ=255)
                                                                                                                                           tCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGAcc      <  3:93119/147‑1 (MQ=255)
                                                                                                                                           tCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACca     >  6:259422/1‑148 (MQ=255)
                                                                                                                                           tCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGAGTGGCGGAAAGGTGGCCGGCAGCGCCCTGGCGCAGATCGAcc      >  4:93119/1‑147 (MQ=255)
                                                                                                                                             gAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACCacac  <  6:107575/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGGAGGTCGCCAGTGTGTTCGGCAAAGCCGGTCGCGCAGAGTCAGCTACCGACCCGGCACCGTTGGAGATGTTCGAGACCATTGTGCGGCTCAAGCCCAAGGATCAGTGGCGGCCTGGAATGAGCAGCGAGAAACTGGTCGAGGAACTCGACCGTACCGTACGCGTACCGGGCCTGACCAACATCTGGATTCCGCCGATCCGCAACCGTATCGACATGCTCGCCACTGGAATCAAGAGCCCGATTGGCGTAAAGGTGGCCGGCAGCGACCTGGCGCAGATCGACCACAC  >  NC_002947/6142062‑6142351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑