Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 532,676 G→T 5.4% intergenic (+19/‑60) PP_t03 → / → tufA tRNA‑Thr/elongation factor Tu‑A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947532,6760GT 5.4% 236.2 / 2.9 74intergenic (+19/‑60)PP_t03/tufAtRNA‑Thr/elongation factor Tu‑A
Reads supporting (aligned to +/- strand):  ref base G (38/31);  new base T (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

TTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCACTCTGACC  >  NC_002947/532530‑532822
                                                                                                                                                  |                                                                                                                                                   
ttCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGa                                                                                                                                                    >  6:341865/1‑148 (MQ=255)
     ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAatat                                                                                                                                              <  1:314637/149‑1 (MQ=255)
         cGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAATTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGc                                                                                                                                          >  5:140505/1‑149 (MQ=255)
         cGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGc                                                                                                                                          >  7:43774/1‑149 (MQ=255)
         cGCTACCCCCCCCCAGTTCGCCGGATGTTCCACACCGTGTGCCGCTCGTGTAGCGCACCCGGGAGCGCACACCCTTCGTAAGCGTGAGGGCAGCGGTCCAAGTCCGCTCAAGAGCTCCATATAAACAACCCAGATATCAAAATATCTGc                                                                                                                                          <  4:88548/149‑1 (MQ=255)
              cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTg                                                                                                                                     >  8:195019/1‑149 (MQ=255)
               ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGt                                                                                                                                    >  8:248972/1‑149 (MQ=255)
                 gCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGttt                                                                                                                                  <  4:339133/149‑1 (MQ=255)
                     cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                                                                               >  1:109467/1‑148 (MQ=255)
                     cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                                                                               >  1:235594/1‑148 (MQ=255)
                     cAAGTTTCTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                                                                               >  3:189824/1‑148 (MQ=255)
                        gTTTGTCGGATTTTGCACAAAGTGTTTCGCACTTGTAGCTCAGTTGGTAGAGCACACCATAGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATATGCCTTTTTATAATCAGt                                                                                                                           >  3:333335/1‑149 (MQ=255)
                          ttGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTTGTAAGGGTGAGGTCAGCGGTTCAACTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTg                                                                                                                          <  6:104134/148‑1 (MQ=255)
                             tCGGATTTTGCACAAAGTGTTACGCACTAGTAGCTCAGTTGGTAGAGCACACACTTGCGAAGGGTGAGGTCAGCGCTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCAGCCGTTGTTTTATCAGTGc                                                                                                                         >  6:17229/1‑146 (MQ=255)
                                       cacaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATTAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttctt                                                                                                            <  5:250742/149‑1 (MQ=255)
                                       cacaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttctt                                                                                                            >  3:331146/1‑149 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                                                                                >  6:338439/1‑126 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                                                                                <  5:338439/126‑1 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGt                                                                                                                           <  7:283834/131‑1 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGt                                                                                                                           >  8:283834/1‑131 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                                                                                         <  7:248972/149‑1 (MQ=255)
                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                                                                                         <  7:332681/149‑1 (MQ=255)
                                             gtgtTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGCCtt                                                                                                                                       <  2:216709/116‑1 (MQ=255)
                                             gtgtTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGCCtt                                                                                                                                       >  1:216709/1‑116 (MQ=255)
                                                tttCGCTCTTGTAGCTCAGTTGGGATAGCACACCCATGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCggagg                                                                                                   <  6:103729/149‑1 (MQ=255)
                                                tttCGCTCTTGTAGCTCAGTTCGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTCCAAGACTATTTCTTCTgaggag                                                                                                    >  5:103729/1‑148 (MQ=255)
                                                   cGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAt                                                                                                 <  4:331146/148‑1 (MQ=255)
                                                        ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttc                                                                                                              <  2:124407/130‑1 (MQ=255)
                                                          gTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACt                                                                                                                   >  8:251501/1‑123 (MQ=255)
                                                           tAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                                                                                        <  2:109467/149‑1 (MQ=255)
                                                             gCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                                                                                      <  7:195019/149‑1 (MQ=255)
                                                              cTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGc                                                                                     >  5:181495/1‑149 (MQ=255)
                                                               tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGc                                                                                     >  8:246865/1‑148 (MQ=255)
                                                                 aGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCt                                                                                    <  5:175387/147‑1 (MQ=255)
                                                                 aGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCt                                                                                    >  2:207162/1‑147 (MQ=255)
                                                                 aGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTaa                                                                                  <  6:181495/149‑1 (MQ=255)
                                                                   ttGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCt                                                                                                                                            >  1:204132/1‑89 (MQ=255)
                                                                   ttGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCt                                                                                                                                            <  2:204132/89‑1 (MQ=255)
                                                                      gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAgg                                                                                >  8:249152/1‑146 (MQ=255)
                                                                      gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAgg                                                                                <  7:249152/146‑1 (MQ=255)
                                                                          agCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt                                                                         >  7:154902/1‑149 (MQ=255)
                                                                          agCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGt                                                                          <  7:246865/148‑1 (MQ=255)
                                                                            cacaCCCTTGGTAAGTGTTATGTCAGAGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATTTCTGCGTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGTTTGTATCGATGGCTAAGGAAAAGttt                                                                        <  4:333335/148‑1 (MQ=255)
                                                                            cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt                                                                        >  7:127922/1‑148 (MQ=255)
                                                                                 ccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt                                                                        >  5:51948/1‑143 (MQ=255)
                                                                                 ccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt                                                                        <  6:51948/143‑1 (MQ=255)
                                                                                  cTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑tt                                                                <  8:127922/149‑1 (MQ=255)
                                                                                     ggTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑ttc                                                               <  1:207162/147‑1 (MQ=255)
                                                                                           ggTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑ttcccttccc                                                        >  2:42902/1‑148 (MQ=255)
                                                                                            gTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCa                                                      >  5:197445/1‑149 (MQ=255)
                                                                                                 gTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                                                                                        >  4:85907/1‑111 (MQ=255)
                                                                                                 gTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                                                                                        <  3:85907/111‑1 (MQ=255)
                                                                                                 gTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGtt                                                  >  6:309648/1‑148 (MQ=255)
                                                                                                         ttCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGcc                                                                                                                                         <  4:188191/54‑1 (MQ=21)
                                                                                                         ttCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGcc                                                                                                                                         >  3:188191/1‑54 (MQ=21)
                                                                                                             aGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTa                                     >  3:150554/1‑149 (MQ=255)
                                                                                                              gTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt                                                                         <  5:122934/113‑1 (MQ=255)
                                                                                                              gTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt                                                                         >  6:122934/1‑113 (MQ=255)
                                                                                                                   cTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGATGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACTTCGGCACTATCGGcc                               <  6:140505/149‑1 (MQ=255)
                                                                                                                   cTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGATTCCCTTCCCCACGTTAACGTCGGCACTATCGGc                                <  1:48501/149‑1 (MQ=255)
                                                                                                                     cAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCAc                             <  8:154902/149‑1 (MQ=255)
                                                                                                                      aaGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACg                            >  2:97297/1‑149 (MQ=255)
                                                                                                                           cTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGAc                       >  6:156489/1‑149 (MQ=255)
                                                                                                                           cTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGAc                       >  5:116049/1‑149 (MQ=255)
                                                                                                                               atatAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACg                   <  4:150554/149‑1 (MQ=255)
                                                                                                                                 ataAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACgg                  >  8:329276/1‑148 (MQ=255)
                                                                                                                                  taAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTa                <  8:43774/149‑1 (MQ=255)
                                                                                                                                    aaCAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTaa               <  5:309648/148‑1 (MQ=255)
                                                                                                                                     aCAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGa             >  6:77610/1‑149 (MQ=255)
                                                                                                                                      cAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGAc            >  6:40085/1‑149 (MQ=255)
                                                                                                                                         ggCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCAc         >  4:177858/1‑149 (MQ=255)
                                                                                                                                         ggCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCAc         <  6:197445/149‑1 (MQ=255)
                                                                                                                                                atGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCACTCTGAcc  >  6:46347/1‑149 (MQ=255)
                                                                                                                                                atGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCACTCTGAcc  >  6:40985/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCG‑TTCCCTTCCCCACGTTAACGTCGGCACTATCGGCCACGTTGACCACGGTAAGACCACTCTGACC  >  NC_002947/532530‑532822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑