Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,174,702	G→T	6.5%	intergenic (‑64/+55)	glmU ← / ← atpC	bifunctional N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,174,702	0	G	T	6.5%	189.4 / 3.8	62	intergenic (‑64/+55)	glmU/atpC	bifunctional N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base G (33/25);  new base T (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

CGGGTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCC  >  NC_002947/6174561‑6174844                                                                                                                                               |                                                                                                                                               cGGGTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGa                                                                                                                                         <  5:36629/149‑1 (MQ=255)        aaCACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAcc                                                                                                                                  >  3:292683/1‑149 (MQ=255)            cTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAcc                                                                                                                                  >  8:12896/1‑145 (MQ=255)            cTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAcc                                                                                                                                  <  7:12896/145‑1 (MQ=255)            cTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCttt                                                                                                                              <  4:292683/149‑1 (MQ=255)                    aGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAAt                                                                                                                      <  3:157840/149‑1 (MQ=255)                          gAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCaa                                                                                                                 >  4:105856/1‑148 (MQ=255)                              gcATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCCAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGaa                                                                                                            <  6:58711/149‑1 (MQ=255)                                 tGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGtt                                                                                                         <  4:175419/149‑1 (MQ=255)                                   cGGGTTCGTCGGCCGGCGGCGATAAAAACGATTTCGATGGACATTACTGGCTACCCATCCTGGGCGGTCAGTGTAAGA‑CCGGGGGAGAACACACCAAAAGAAAATGGGTCGCCGAGGCTACCCTTTTACTCAATCTCAACCGAAGTTAt                                                                                                       <  6:345938/149‑1 (MQ=255)                                          cTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGCCTACCCTTTTACTCAATCGCACCAGaa                                                                                                            >  1:172836/1‑137 (MQ=255)                                          cTTGGCCGGCGGCGAGAATAACGATATCGAGTGAAATTAGTTGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACATAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGaa                                                                                                            <  2:172836/137‑1 (MQ=255)                                           ttGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCggg                                                                                               >  4:225203/1‑149 (MQ=255)                                           ttGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCggg                                                                                               >  6:355894/1‑149 (MQ=255)                                              gCCGGCGGCGAGAATAACGATATCGAGTGACATTACTTGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGTAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGc                                                                                             <  5:355894/148‑1 (MQ=255)                                                    ggcgAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTAc                                                                                      <  2:320866/149‑1 (MQ=255)                                                             aaCGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGAtt                                                                                        <  7:291711/138‑1 (MQ=255)                                                             aaCGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGAtt                                                                                        >  8:291711/1‑138 (MQ=255)                                                                   atCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCAt                                                                        >  1:79677/1‑149 (MQ=255)                                                                      gAGTGACATTACTGGGTACCAATCCTGGGCGGTCAGTGAAAGC‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCGACCCTTTTACTCAATCGCAACAGAAGTTTTCAGGCGGGGCCGATTAGTTGCGGTTGCGCATTTGc                                                                    >  6:296286/1‑149 (MQ=255)                                                                      gAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTg                                                                     <  3:105856/148‑1 (MQ=255)                                                                      gAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGc                                                                    >  5:335777/1‑149 (MQ=255)                                                                                       aCCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTg                                                   >  4:147051/1‑149 (MQ=255)                                                                                       aCCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTg                                                   >  7:274425/1‑149 (MQ=255)                                                                                       aCCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTg                                                   >  1:40888/1‑149 (MQ=255)                                                                                        ccAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTgg                                                  >  6:208126/1‑149 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGa                                                                >  7:100774/1‑134 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGa                                                                <  8:100774/134‑1 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTggg                                                 >  3:187801/1‑149 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTggg                                                 >  6:267721/1‑149 (MQ=255)                                                                                          aaTCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGATAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGc                                                >  8:282682/1‑149 (MQ=255)                                                                                               tGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCgg                                           >  8:2358/1‑149 (MQ=255)                                                                                                  gCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCt                                        <  3:225203/149‑1 (MQ=255)                                                                                                  gCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCt                                        <  4:187801/149‑1 (MQ=255)                                                                                                  gCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCCCAGCTGCgtacc                                        <  2:79677/149‑5 (MQ=255)                                                                                                   cGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTc                                       <  2:40888/149‑1 (MQ=255)                                                                                                   cGGTCAGTGAAAGA‑CCGGGGCAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGACTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTc                                       >  5:13499/1‑149 (MQ=255)                                                                                                    ggTCAGTGAAAGA‑CCGGGGGAGATAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCg                                      <  7:282682/149‑1 (MQ=255)                                                                                                        aGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGc                                    >  2:102700/1‑147 (MQ=255)                                                                                                            aaaGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCt                                                    <  6:230637/127‑1 (MQ=255)                                                                                                            aaaGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCt                                                    >  5:230637/1‑127 (MQ=255)                                                                                                                   cGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgcggc                        >  6:32281/1‑149 (MQ=255)                                                                                                                      gggAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgcggcggc                     <  7:2358/149‑1 (MQ=255)                                                                                                                          gaAAACATAAACAGAAAAAGGGTTGCGGAGGCTACCCTTTTACTCAATATCAACAGAAGTTATCAGCGGGGGCCGATTACTTGCCTTTGCGCTTTTGGTGGACGGTACGCAGCTGGGCTGCGGCCTCgggc                                   <  6:195832/131‑3 (MQ=255)                                                                                                                           aaaaCAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGgcgc                >  2:54911/1‑149 (MQ=255)                                                                                                                            aaaCAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGcc               <  5:296286/149‑1 (MQ=255)                                                                                                                             aaCAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGGTGGGCTGCGGCCTCGGCGAGACGTGCGGCGGCGgcgcgg              >  8:270915/1‑147 (MQ=255)                                                                                                                             aaCAGAAAAAGAAAAAGGGTAGCCGAGCCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGTTCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCg              <  1:282695/149‑1 (MQ=255)                                                                                                                                agaaaaagaaaaAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACTGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAg           >  7:242017/1‑149 (MQ=255)                                                                                                                                agaaaaagaaaaAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAg           >  5:278067/1‑149 (MQ=255)                                                                                                                                agaaaaagaaaaAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAg           >  1:362008/1‑149 (MQ=255)                                                                                                                                  aaaaagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTc         <  5:32281/149‑1 (MQ=255)                                                                                                                                    aaagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCg        >  6:17280/1‑148 (MQ=255)                                                                                                                                     aagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCg        >  2:242419/1‑147 (MQ=255)                                                                                                                                     aagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGATGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCgcgatcgg       >  3:38577/1‑141 (MQ=255)                                                                                                                                        taaaagGTTAGCCTCGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTgg                                                  <  3:191388/101‑1 (MQ=255)                                                                                                                                        taaaagGTTAGCCTCGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTgg                                                  <  3:186791/101‑1 (MQ=255)                                                                                                                                        taaaagGTTAGCCTCGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTgg                                                  >  4:186791/2‑102 (MQ=255)                                                                                                                                        taaaagGTTAGCCTCGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTgg                                                  >  4:191388/2‑102 (MQ=255)                                                                                                                                         aaaagGGTAGCCTCGGCTACCCGTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTcc  >  2:225943/1‑149 (MQ=255)                                                                                                                                          aaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGc                                             <  2:82841/105‑1 (MQ=255)                                                                                                                                          aaagGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGc                                             >  1:82841/1‑105 (MQ=255)                                                                                                                                               |                                                                                                                                               CGGGTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGA‑CCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATCGCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCC  >  NC_002947/6174561‑6174844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑