Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 635,841 A→T 8.2% E22V (GAA→GTA) ‡ mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947635,8410AT 8.2% 142.7 / 3.3 49E22V (GAA→GTA) ‡mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CAGGGGCGACTGAGCGAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCGGCAACGC  >  NC_002947/635698‑635985
                                                                                                                                               |                                                                                                                                                
cAGGGGCGACTGAGCGAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTg                                                                                                                                             <  5:109125/149‑1 (MQ=255)
 aGGGGCGACTGAGCGAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTgg                                                                                                                                            <  3:276612/149‑1 (MQ=255)
            aGCGAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACCTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCa                                                                                                                                 >  5:332219/1‑149 (MQ=255)
               gAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACcg                                                                                                                                      <  3:190943/141‑1 (MQ=255)
               gAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACcg                                                                                                                                      >  4:190943/1‑141 (MQ=255)
                 aaCAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCgg                                                                                                                             <  4:216629/148‑1 (MQ=255)
                       gggTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGAc                                                                                                                      >  6:142999/1‑149 (MQ=255)
                            cGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCa                                                                                                                  <  4:136173/148‑1 (MQ=255)
                             gATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGTAACTTGGCCACCGCGTCACCGGCTCTGACGCCAAc                                                                                                                <  6:247122/149‑1 (MQ=255)
                             gATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACCTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAAc                                                                                                                <  6:332219/149‑1 (MQ=255)
                                ttGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTc                                                                                                             >  1:104315/1‑149 (MQ=255)
                                  gTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCt                                                                                                            <  2:326680/148‑1 (MQ=255)
                                     gcgcCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATcc                                                                                                        >  3:217621/1‑149 (MQ=255)
                                             ggCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATg                                                                                                 <  4:69565/148‑1 (MQ=255)
                                             ggCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGGTCGCTGGCGGGGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGa                                                                                                <  8:315807/149‑1 (MQ=255)
                                               cTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCAc                                                                                                                                >  1:231621/1‑115 (MQ=255)
                                               cTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCAc                                                                                                                                <  2:231621/115‑1 (MQ=255)
                                               cTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGt                                                                                                              >  4:197892/1‑133 (MQ=255)
                                               cTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGt                                                                                                              <  3:197892/133‑1 (MQ=255)
                                                                     gAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTa                                                                        >  8:169009/1‑149 (MQ=255)
                                                                             ccATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTg                                                                <  5:288836/149‑1 (MQ=255)
                                                                                tGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGa                                                               >  6:9222/1‑147 (MQ=255)
                                                                                tGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGa                                                               <  5:9222/147‑1 (MQ=255)
                                                                                      ttCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGc                                                                                              >  1:35351/1‑110 (MQ=255)
                                                                                       tCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCATCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGaa                                                                   <  4:168884/136‑1 (MQ=255)
                                                                                       tCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCATCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGaa                                                                   >  3:168884/1‑136 (MQ=255)
                                                                                         acaTTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAgg                                                        >  4:162410/1‑145 (MQ=255)
                                                                                         acaTTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAgg                                                        <  3:162410/145‑1 (MQ=255)
                                                                                         acaTTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTAt                                                    >  6:227298/1‑149 (MQ=255)
                                                                                                 ggTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGc                                                                                       >  8:260620/1‑106 (MQ=255)
                                                                                                 ggTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGc                                                                                       <  7:260620/106‑1 (MQ=255)
                                                                                                  gTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGcc                                           <  5:142999/149‑1 (MQ=255)
                                                                                                       tGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGc                                       <  2:104315/148‑1 (MQ=255)
                                                                                                        gCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCt                                      <  7:54570/148‑1 (MQ=255)
                                                                                                        gCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCt                                      <  4:217621/148‑1 (MQ=255)
                                                                                                                 tCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGgc                             <  7:169009/148‑1 (MQ=255)
                                                                                                                       gTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCt                                      >  8:242926/1‑133 (MQ=255)
                                                                                                                       gTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCt                                      <  7:242926/133‑1 (MQ=255)
                                                                                                                                 ggTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCa            >  7:91003/1‑149 (MQ=255)
                                                                                                                                     gtGGCCAAGTTCCTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCgg        <  3:264933/148‑1 (MQ=255)
                                                                                                                                     gtGGCCAAGTTCCTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCgg        >  4:264933/2‑149 (MQ=255)
                                                                                                                                     gtGGCCAAGTTCCTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCg         >  6:33602/2‑148 (MQ=255)
                                                                                                                                     gtGGCCAAGTTCCTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCg         <  5:33602/147‑1 (MQ=255)
                                                                                                                                       ggCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCGGc       >  2:120712/1‑148 (MQ=255)
                                                                                                                                        gCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCtgg                  <  4:246848/136‑1 (MQ=255)
                                                                                                                                        gCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCtgg                  >  3:246848/1‑136 (MQ=255)
                                                                                                                                          cAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCtggtg                >  6:5971/1‑136 (MQ=255)
                                                                                                                                          cAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCtggtg                <  5:5971/136‑1 (MQ=255)
                                                                                                                                           aaGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATACGGCGCCAGACCGGGTGGTCATCCGCAACGc  >  6:210555/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CAGGGGCGACTGAGCGAAACAGCGGGTGCGATTTGTCGCGCCCTGGGCTACCCTGCCTCGATGTTTTGCGAGGTCGACCATGCATATTCACATTCTCGGTATTTGCGGCACTTTCATGGGTTCGCTGGCGGTGCTGGCCAAGGAACTTGGCCACCGCGTCACCGGCTCTGACGCCAACGTCTATCCCCCGATGAGCACCCAGCTCGAAGCCCAGGGTATCGAACTGACCCAAGGCTATGACCCGGCCCAGCTGGATCCGGCGCCAGACCTGGTGGTCATCGGCAACGC  >  NC_002947/635698‑635985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑