Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	90,803	C→G	5.9%	G88R (GGG→CGG)	PP_0080 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	90,803	0	C	G	5.9%	210.3 / 3.5	68	G88R (GGG→CGG)	PP_0080	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (36/28);  new base G (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

CCATCAACCACCTGGGCCACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAGG  >  NC_002947/90655‑90942                                                                                                                                                     |                                                                                                                                            ccATCAACCACCTGGGCCACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGccc                                                                                                                                             <  3:138079/149‑1 (MQ=255) ccATCAACCACCAGAGCCACGCATTCACGATACACCTCTCTCTACTAGCCCCATTCATCCAGCACATCTAAAACACCCACAAGACCAAGACGACGAATAGTCGTACGCGCAAGCCACGAAACCAGCGCCTCGCTCAAACGTGCCTcccc                                                                                                                                             >  4:138079/1‑149 (MQ=255)                 ccACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCGGGGCAggcacgctt                                                                                                                               <  7:101864/147‑8 (MQ=255)                 ccACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCGGGGCAggcacgctt                                                                                                                               >  8:101864/1‑140 (MQ=255)                 ccACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGgc                                                                                                                             <  7:94633/149‑1 (MQ=255)                  cACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCAACCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGAATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTCCCCCGGCAGGTCACCCGgcg                                                                                                                            >  1:158530/1‑149 (MQ=255)                   aCGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGgcgc                                                                                                                           >  1:12124/1‑149 (MQ=255)                     gCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCa                                                                                                                         <  2:12124/149‑1 (MQ=255)                           ccGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTg                                                                                                                   >  6:30640/1‑149 (MQ=255)                             gCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTgg                                                                                                                  >  4:294373/1‑148 (MQ=255)                                  aCCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAg                                                                                                            >  3:199821/1‑149 (MQ=255)                                   ccTCGCTGTACTTGGCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCAc                                                                                                                                  >  7:305941/1‑126 (MQ=255)                                   ccTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCAc                                                                                                                                  <  8:305941/126‑1 (MQ=255)                                   ccTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAgg                                                                                                           <  7:86979/149‑1 (MQ=255)                                    cTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAggg                                                                                                          >  3:198813/1‑149 (MQ=255)                                    cTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAggg                                                                                                          <  3:294373/149‑1 (MQ=255)                                      cGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCGGGGCAggcac                                                                                                                                   >  5:350845/1‑119 (MQ=255)                                      cGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCGGGGCAggcac                                                                                                                                   <  6:350845/122‑4 (MQ=255)                                        cTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCCGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGAt                                                                                                      <  7:51572/149‑1 (MQ=255)                                           tACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATc                                                                                                     <  4:94541/147‑1 (MQ=255)                                              ttGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAggg                                                                                                          >  2:20237/1‑139 (MQ=255)                                              ttGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAggg                                                                                                          <  1:20237/139‑1 (MQ=255)                                                    ggTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAgcc                                                                                          >  1:334926/1‑149 (MQ=255)                                                    ggTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAgcc                                                                                          >  1:340277/1‑149 (MQ=255)                                                     gTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAgccg                                                                                         >  2:146194/1‑149 (MQ=255)                                                      ttCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAgccgc                                                                                        >  4:194432/1‑149 (MQ=255)                                                          tCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGccgcccgc                                                                                    >  1:81286/1‑149 (MQ=255)                                                          tCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGccgcccgc                                                                                    >  2:150515/1‑149 (MQ=255)                                                          tCCAGCACATCGAAATCGCCCAAAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGccgcccgc                                                                                    >  7:53358/1‑149 (MQ=255)                                                              gCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTgcg                                                                                >  7:247727/1‑149 (MQ=255)                                                                  aTCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTg                                                                                                                   <  4:71760/110‑1 (MQ=255)                                                                  aTCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTg                                                                                                                   >  3:71760/1‑110 (MQ=255)                                                                  aTCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCAccgc                                                                                                >  3:237274/1‑129 (MQ=255)                                                                  aTCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCAccgc                                                                                                <  4:237274/129‑1 (MQ=255)                                                                      aaaTCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTc                                                                        >  6:264546/1‑149 (MQ=255)                                                                       aaTCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTcc                                                                       >  6:81209/1‑149 (MQ=255)                                                                         tCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGc                                                                     <  5:81209/149‑1 (MQ=255)                                                                            cccACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGcc                                                                              >  2:42173/1‑137 (MQ=255)                                                                            cccACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGcc                                                                              <  1:42173/137‑1 (MQ=255)                                                                               acaAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCtggtg                                                               <  1:150515/149‑1 (MQ=255)                                                                                    cagcagcCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTc                                                                        >  8:166800/1‑135 (MQ=255)                                                                                    cagcagcCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTc                                                                        <  7:166800/135‑1 (MQ=255)                                                                                     agcagcCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCAGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCAt                                                          >  3:132440/1‑148 (MQ=255)                                                                                      gcagcCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCa                                                        >  3:59930/1‑149 (MQ=255)                                                                                             gCGTATAGTCGTACGCGCAAGCCCCGCCTCCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGcc                                                 >  1:203284/1‑149 (MQ=255)                                                                                               gTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGcccc                                               <  8:247727/149‑1 (MQ=255)                                                                                                   aGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCt                                            >  5:352874/1‑148 (MQ=255)                                                                                                     tCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATc                                         <  5:30640/149‑1 (MQ=255)                                                                                                     gcGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTAt                                          <  3:222403/147‑1 (MQ=255)                                                                                                        tACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCtt                                       >  6:89708/1‑148 (MQ=255)                                                                                                          cgcgCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGaa                                    <  5:89708/149‑1 (MQ=255)                                                                                                          cgcgCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGaa                                    <  2:158530/149‑1 (MQ=255)                                                                                                             gcAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTg                                 >  3:76709/1‑149 (MQ=255)                                                                                                                  ccccGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTTCGCCTGGTGGGCATCACTCAGCTCCCCTATCTTGAAGTGCAGGc                            <  8:53358/149‑1 (MQ=255)                                                                                                                     cGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTGACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCg                         >  8:69433/1‑149 (MQ=255)                                                                                                                      gCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCGTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCgg                        >  5:369057/1‑149 (MQ=255)                                                                                                                       ccaccaGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCggg                       <  6:352874/149‑1 (MQ=255)                                                                                                                            aGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCgcgg                  >  5:347861/1‑149 (MQ=255)                                                                                                                                ccTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGaa              <  4:76709/149‑1 (MQ=255)                                                                                                                                 cTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAAt             >  8:63040/1‑149 (MQ=255)                                                                                                                                     cTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGGCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCgg                        <  1:254924/134‑1 (MQ=255)                                                                                                                                     cTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCgg                        >  2:254924/1‑134 (MQ=255)                                                                                                                                      tCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCgg        >  5:49476/1‑149 (MQ=255)                                                                                                                                       cAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGt       >  5:261113/1‑149 (MQ=255)                                                                                                                                        aaGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGtt      >  3:9650/1‑149 (MQ=255)                                                                                                                                           cGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAg   <  5:264546/149‑1 (MQ=255)                                                                                                                                           cGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAg   <  4:59930/149‑1 (MQ=255)                                                                                                                                           cGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAg   <  3:194432/149‑1 (MQ=255)                                                                                                                                           cGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAg   <  1:146194/149‑1 (MQ=255)                                                                                                                                            gTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAgg  <  6:49476/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            CCATCAACCACCTGGGCCACGCATTCCCGCTCCACCTCGCTGTACTTGCCCGGTTCATCCAGCACATCGAAATCGCCCACAAGCAGCAGCCGGCGTATAGTCGTACGCGCAAGCCCCGCCACCAGCGCCTCGCTCAAGCGTGCCTGCCCCGGCAGGTCACCCGGCGCCAGCGCTGGTAACAGGGCGATCACCGCCGAGCCGCCCGCTGCGCCCTGCTCCGCCTGGTGGGCATCACCCAGCCCCCCTATCTTGAAGTGCAGGCCCGGGCGCGGCGAATGGCGGTTGAGG  >  NC_002947/90655‑90942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑