Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,112	C→A	10.2%	G99G (GGG→GGT) ‡	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,112	0	C	A	10.2%	211.7 / 11.0	76	G99G (GGG→GGT) ‡	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (35/33);  new base A (5/3);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

GCGAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAG  >  NC_002947/99965‑100258                                                                                                                                                     |                                                                                                                                                   gCGAAACAGCTCGACCTTGTTGGTGCGCGGCCCGTCCAGTTCCCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAACGGGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGGGGCAATGTACAGCGTGCGGCCCTGc                                                                                                                                                    <  8:129757/148‑1 (MQ=255)         gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                           >  3:317342/1‑149 (MQ=255)          cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                          <  6:234081/149‑1 (MQ=255)           tCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         >  4:132917/1‑149 (MQ=255)               ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                      >  3:125939/1‑148 (MQ=255)                cTTGTTGGTGCGCGTGCGGTCCAGTTCACTGCAGTAGATCACCTTGAAGTGATGATCGATCGCAAAGTGCCGAGCGATTTCGAGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGCCGCa                                                                                                                                     <  8:240637/148‑1 (MQ=255)                cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                      >  8:190848/1‑147 (MQ=255)                cTTGTTGGTGCGCGTGACGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGCTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCACGTTCAGGGcagggg                                                                                                                                    >  6:182698/1‑144 (MQ=255)                   gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                  <  7:190848/148‑1 (MQ=255)                    ttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGTGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCa                                                                                                                                 <  1:211591/148‑1 (MQ=255)                       gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                             >  4:279406/1‑149 (MQ=255)                        tGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTcc                                                                                                                            <  2:291984/149‑1 (MQ=255)                                  gTCCTGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGAGTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                  <  7:334192/149‑1 (MQ=255)                                  gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                  <  8:183902/149‑1 (MQ=255)                                   tCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTc                                                                                                                 >  8:23439/1‑149 (MQ=255)                                           aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                           >  1:195420/1‑147 (MQ=255)                                            cTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGCCGAACTCCCATGGTTT‑GGAGGTCGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACATTCGAACACGAg                                                                                                        >  5:43996/1‑149 (MQ=255)                                               ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                     >  5:56540/1‑149 (MQ=255)                                                  tAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                  <  5:153842/149‑1 (MQ=255)                                                       cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  2:211697/1‑147 (MQ=255)                                                       cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  1:204950/1‑147 (MQ=255)                                                        aCCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                             <  6:56540/148‑1 (MQ=255)                                                          cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                          >  5:10548/1‑149 (MQ=255)                                                          cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                          <  6:10548/149‑1 (MQ=255)                                                           ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCt                                                                                                                   <  4:25286/123‑1 (MQ=255)                                                           ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCt                                                                                                                   >  3:25286/1‑123 (MQ=255)                                                              aaGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         >  7:34600/1‑98 (MQ=255)                                                              aaGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         <  8:34600/98‑1 (MQ=255)                                                                 tgatgaTAGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGACCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAc                                                                                   >  7:235655/1‑149 (MQ=255)                                                                  gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                         >  7:242099/1‑126 (MQ=255)                                                                  gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                         <  8:242099/126‑1 (MQ=255)                                                                   atgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgc                                                                                 <  1:172872/149‑1 (MQ=255)                                                                     gatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgcg                                                                                >  1:265536/1‑149 (MQ=255)                                                                       tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGa                                                                              <  4:125939/148‑1 (MQ=255)                                                                          aaCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAGGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcg                                                                          >  2:69774/1‑149 (MQ=255)                                                                              cAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  6:91844/1‑124 (MQ=255)                                                                              cAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               <  5:91844/124‑1 (MQ=255)                                                                               aaaGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGTAATGTACACCGTGCGCCCCTCCCCGTTCAGGGCCGCAAGCAACTCCGCCACACCAACGAACACGAGTGTTACGTAAAGACCGGACCCACGGAAgcgaacgc                                                                     >  8:236763/1‑144 (MQ=255)                                                                                      cGAGCGATTTCGCGGGCGAACTCCCATGGGTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                              <  4:11707/149‑1 (MQ=255)                                                                                        aGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGt                                                            >  5:178309/1‑149 (MQ=255)                                                                                             tttCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  3:279406/149‑1 (MQ=255)                                                                                              ttCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCTCACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  3:132917/148‑1 (MQ=255)                                                                                                 gcccgCGAAATCGCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCg                                                                                        >  6:29689/5‑112 (MQ=255)                                                                                                     gCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATc                                                  >  8:261908/1‑146 (MQ=255)                                                                                                     gCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATc                                                  <  7:261908/146‑1 (MQ=255)                                                                                                       gAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                     >  1:128551/1‑125 (MQ=255)                                                                                                       gAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                     <  2:128551/125‑1 (MQ=255)                                                                                                        aaCTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGc                                            <  7:23439/149‑1 (MQ=255)                                                                                                           tCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGTCtt                                         >  8:315567/1‑149 (MQ=255)                                                                                                             ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                       <  1:69774/149‑1 (MQ=255)                                                                                                             ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                       >  7:313455/1‑149 (MQ=255)                                                                                                             ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                        <  2:195420/148‑1 (MQ=255)                                                                                                              cATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCt                                          >  4:255630/1‑145 (MQ=255)                                                                                                              cATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCt                                          <  3:255630/145‑1 (MQ=255)                                                                                                                tGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTTAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCGCAGGCCTTGGCtt                                    >  3:43506/1‑149 (MQ=255)                                                                                                                            tGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                         <  2:265536/149‑1 (MQ=255)                                                                                                                              gCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGt                        <  8:235655/148‑1 (MQ=255)                                                                                                                                aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  7:193100/115‑1 (MQ=255)                                                                                                                                aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       >  8:193100/1‑115 (MQ=255)                                                                                                                                aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAg                      >  4:85878/1‑148 (MQ=255)                                                                                                                                   gTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                       >  3:149582/1‑144 (MQ=255)                                                                                                                                   gTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                       <  4:149582/144‑1 (MQ=255)                                                                                                                                      cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               <  6:178309/149‑1 (MQ=255)                                                                                                                                        gCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              >  7:160754/1‑148 (MQ=255)                                                                                                                                          ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               <  5:66619/141‑1 (MQ=255)                                                                                                                                          ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               >  6:66619/5‑145 (MQ=255)                                                                                                                                          ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              <  1:151440/142‑1 (MQ=255)                                                                                                                                          ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              >  2:151440/5‑146 (MQ=255)                                                                                                                                          ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa            >  5:55325/5‑148 (MQ=255)                                                                                                                                           tGCGGCCCTGCCAGTTCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa          <  7:236763/149‑1 (MQ=255)                                                                                                                                             cGGACCTTCACGTTCATGGCCTCAAGCAACTCCGGCACACCTTCGAACACGATGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc        <  4:43506/149‑1 (MQ=255)                                                                                                                                              ggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGcc       >  8:122653/1‑149 (MQ=255)                                                                                                                                                 ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc    <  8:160754/149‑1 (MQ=255)                                                                                                                                                 ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg     >  7:238948/1‑148 (MQ=255)                                                                                                                                                 ccTGCCAGTTCATGGCCTCAATCAAATCCGTCACACCTTCGAACAATAGTTTTTAGTAAAGAACGGTCAAGCTTAAGATCTCGCTGTAGAAGTTCACCTCATCCAAGGCCTTGGCTTATTAGAAGCTGTAGAACTGCATGAAAgaatgc    <  4:317342/149‑6 (MQ=255)                                                                                                                                                    gCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag  >  6:74689/1‑148 (MQ=255)                                                                                                                                                    gCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag  >  2:296892/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCGAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAG  >  NC_002947/99965‑100258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑