Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 806,078 T→G 6.3% M418L (ATG→CTG)  PP_0693 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947806,0780TG 6.3% 227.9 / 4.4 74M418L (ATG→CTG) PP_0693hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/33);  new base G (3/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CCAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCG  >  NC_002947/805937‑806225
                                                                                                                                             |                                                                                                                                                   
ccAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACACACGCATCAGGCgg                                                                                                                                              >  1:321585/1‑149 (MQ=255)
  aGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTc                                                                                                                                            <  4:238477/149‑1 (MQ=255)
   ggCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTc                                                                                                                                            >  3:215746/1‑148 (MQ=255)
    gCCTTGGGCCTCGAATCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACACACAGGTCGCTGTCGTCCAGCTCGATAACGCGATCTCCCAGCGGTAGGCTTAACAGCGCTCCGTTGAACAGACGCTTCAGGCGGTc                                                                                                                                            <  6:321934/147‑1 (MQ=255)
                  aGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCgag                                                                                                                            <  1:173926/149‑1 (MQ=255)
                      ttagCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACATGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACCGCGCGCCGTGGAACAGACGCAGCAGGCGGTCGACGGCctgctga                                                                                                                               >  8:286322/4‑141 (MQ=255)
                      ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCctgctg                                                                                                                                >  4:82829/1‑141 (MQ=255)
                      ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCctgctg                                                                                                                                <  3:82829/141‑1 (MQ=255)
                      ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCAGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAAct                                                                                                                        >  3:338272/1‑149 (MQ=255)
                      tgcGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCtgctgc                                                                                                                               <  7:286322/140‑1 (MQ=255)
                          cccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTc                                                                                                                    >  6:56206/1‑149 (MQ=255)
                           ccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGCCTGACCACCGCGCCGTTGAACAGACGCATCAGGCGGACGACGTCCTGCTGCGAGAACTCTTcg                                                                                                                   >  5:273160/1‑149 (MQ=255)
                             ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                        <  7:324237/126‑1 (MQ=255)
                             ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                        >  8:324237/1‑126 (MQ=255)
                               gAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTcgcg                                                                                                                 >  3:97472/1‑147 (MQ=255)
                                 aGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAgg                                                                                                             >  4:183215/1‑149 (MQ=255)
                                    cGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCgg                                                                                                          <  6:105211/149‑1 (MQ=255)
                                     gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                         <  1:342352/149‑1 (MQ=255)
                                     gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                         <  3:183215/149‑1 (MQ=255)
                                          gCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACACACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAg                                                                                                    <  2:321585/149‑1 (MQ=255)
                                             ccGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtg                                                                                                 >  5:320575/1‑149 (MQ=255)
                                               gCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTc                                                                                                                                            <  5:271183/104‑1 (MQ=255)
                                               gCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTc                                                                                                                                            >  6:271183/1‑104 (MQ=255)
                                               gCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgtt                                                                                               <  5:56206/149‑1 (MQ=255)
                                                   ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                          >  7:44232/1‑118 (MQ=255)
                                                   ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                          <  8:44232/118‑1 (MQ=255)
                                                     aGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                          >  2:250581/1‑148 (MQ=255)
                                                     aGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCAGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                          >  3:172185/1‑148 (MQ=255)
                                                        gATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGc                                                                                      <  6:16766/149‑1 (MQ=255)
                                                         aTTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCg                                                                                     >  1:92153/1‑149 (MQ=255)
                                                                ccACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATc                                                                                >  4:236083/1‑147 (MQ=255)
                                                                  acaGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgc                                                                            >  1:16105/1‑149 (MQ=255)
                                                                      gTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgctgct                                                                        >  1:29444/1‑149 (MQ=255)
                                                                        cGCTGTCTTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgctgctgc                                                                      <  6:320575/149‑1 (MQ=255)
                                                                                 ccAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGc                                                             >  2:147152/1‑149 (MQ=255)
                                                                                   aGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCtt                                                           >  8:107950/1‑149 (MQ=255)
                                                                                   aGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCtt                                                           >  7:26886/1‑149 (MQ=255)
                                                                                    gCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTc                                                          <  6:273160/149‑1 (MQ=255)
                                                                                    gCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTc                                                          <  2:92153/149‑1 (MQ=255)
                                                                                     cTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCa                                                         <  3:236083/149‑1 (MQ=255)
                                                                                        gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAgg                                                       <  7:107950/148‑1 (MQ=255)
                                                                                        gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAg                                                        <  4:97472/147‑1 (MQ=255)
                                                                                           aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                 >  4:132672/1‑119 (MQ=255)
                                                                                           aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                 <  3:127498/119‑1 (MQ=255)
                                                                                           aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                 >  4:127498/1‑119 (MQ=255)
                                                                                           aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                 <  3:132672/119‑1 (MQ=255)
                                                                                                    tcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGt                                          >  4:203963/1‑149 (MQ=255)
                                                                                                      ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                        >  5:356923/1‑149 (MQ=255)
                                                                                                      ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                        >  6:132931/1‑149 (MQ=255)
                                                                                                      ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGAGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                        >  1:269777/1‑149 (MQ=255)
                                                                                                      ccAGCAGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCa                                             <  4:338272/144‑1 (MQ=255)
                                                                                                        aGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcgcg                                      <  6:356923/149‑1 (MQ=255)
                                                                                                            gCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                         >  2:353630/1‑78 (MQ=255)
                                                                                                            gCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                         <  1:353630/78‑1 (MQ=255)
                                                                                                                  tGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGc                                                      <  2:33031/123‑1 (MQ=255)
                                                                                                                  tGAACAGCGCGCCGTTGAACAGACGCATCAAGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGc                                                      >  1:33031/1‑123 (MQ=255)
                                                                                                                    aaCAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCtcgtc                          >  7:42097/1‑149 (MQ=255)
                                                                                                                       aGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAg                       <  2:269777/149‑1 (MQ=255)
                                                                                                                        gcgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAgg                      <  1:147152/149‑1 (MQ=255)
                                                                                                                        gcgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAgg                      >  7:27906/1‑149 (MQ=255)
                                                                                                                         cgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGt                     >  2:208990/1‑149 (MQ=255)
                                                                                                                          gcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTAcg                                  <  1:235030/135‑1 (MQ=255)
                                                                                                                          gcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTAcg                                  >  2:235030/1‑135 (MQ=255)
                                                                                                                                   aaCAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACt           <  5:132931/149‑1 (MQ=255)
                                                                                                                                      aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTTACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGaa        <  4:172185/149‑1 (MQ=255)
                                                                                                                                      aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGa         >  7:118522/1‑148 (MQ=255)
                                                                                                                                        tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                    <  8:157327/86‑1 (MQ=37)
                                                                                                                                        tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                    >  7:152595/2‑87 (MQ=37)
                                                                                                                                        tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                    <  8:152595/86‑1 (MQ=37)
                                                                                                                                        tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                    >  7:157327/2‑87 (MQ=37)
                                                                                                                                          gCATCAGGCGGTCGTCGTCCTGCTGCGAGATGTCTTCGCTCTGGCGGGCATATCTGTTGTTGTCAGCTTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCa                                             <  5:238492/108‑1 (MQ=255)
                                                                                                                                           cATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGcc   <  8:118522/149‑1 (MQ=255)
                                                                                                                                            aTCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCg  >  3:236654/1‑149 (MQ=255)
                                                                                                                                            aTCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCg  >  4:347598/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCG  >  NC_002947/805937‑806225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑