Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	806,091	G→C	5.7%	D413E (GAC→GAG) ‡	PP_0693 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	806,091	0	G	C	5.7%	221.9 / 4.0	70	D413E (GAC→GAG) ‡	PP_0693	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

AGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCA  >  NC_002947/805955‑806236                                                                                                                                         |                                                                                                                                                   aGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCgag                                                                                                                                        <  1:173926/149‑1 (MQ=255)     ttagCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACATGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACCGCGCGCCGTGGAACAGACGCAGCAGGCGGTCGACGGCctgctga                                                                                                                                           >  8:286322/4‑141 (MQ=255)     ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCctgctg                                                                                                                                            >  4:82829/1‑141 (MQ=255)     ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCctgctg                                                                                                                                            <  3:82829/141‑1 (MQ=255)     ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCAGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAAct                                                                                                                                    >  3:338272/1‑149 (MQ=255)     tgcGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCtgctgc                                                                                                                                           <  7:286322/140‑1 (MQ=255)         cccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTc                                                                                                                                >  6:56206/1‑149 (MQ=255)          ccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGCCTGACCACCGCGCCGTTGAACAGACGCATCAGGCGGACGACGTCCTGCTGCGAGAACTCTTcg                                                                                                                               >  5:273160/1‑149 (MQ=255)            ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                                    <  7:324237/126‑1 (MQ=255)            ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                                    >  8:324237/1‑126 (MQ=255)              gAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTcgcg                                                                                                                             >  3:97472/1‑147 (MQ=255)                aGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAgg                                                                                                                         >  4:183215/1‑149 (MQ=255)                   cGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCgg                                                                                                                      <  6:105211/149‑1 (MQ=255)                    gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                     <  1:342352/149‑1 (MQ=255)                    gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                     <  3:183215/149‑1 (MQ=255)                         gCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACACACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAg                                                                                                                <  2:321585/149‑1 (MQ=255)                            ccGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtg                                                                                                             >  5:320575/1‑149 (MQ=255)                              gCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgtt                                                                                                           <  5:56206/149‑1 (MQ=255)                                  ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                                      >  7:44232/1‑118 (MQ=255)                                  ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                                      <  8:44232/118‑1 (MQ=255)                                    aGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                      >  2:250581/1‑148 (MQ=255)                                    aGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCAGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                      >  3:172185/1‑148 (MQ=255)                                       gATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGc                                                                                                  <  6:16766/149‑1 (MQ=255)                                        aTTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCg                                                                                                 >  1:92153/1‑149 (MQ=255)                                               ccACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATc                                                                                            >  4:236083/1‑147 (MQ=255)                                                 acaGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgc                                                                                        >  1:16105/1‑149 (MQ=255)                                                     gTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgctgct                                                                                    >  1:29444/1‑149 (MQ=255)                                                       cGCTGTCTTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgctgctgc                                                                                  <  6:320575/149‑1 (MQ=255)                                                                ccAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGc                                                                         >  2:147152/1‑149 (MQ=255)                                                                  aGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCtt                                                                       >  8:107950/1‑149 (MQ=255)                                                                  aGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCtt                                                                       >  7:26886/1‑149 (MQ=255)                                                                   gCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTc                                                                      <  6:273160/149‑1 (MQ=255)                                                                   gCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTc                                                                      <  2:92153/149‑1 (MQ=255)                                                                    cTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCa                                                                     <  3:236083/149‑1 (MQ=255)                                                                       gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAgg                                                                   <  7:107950/148‑1 (MQ=255)                                                                       gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAg                                                                    <  4:97472/147‑1 (MQ=255)                                                                          aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                             >  4:127498/1‑119 (MQ=255)                                                                          aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                             >  4:132672/1‑119 (MQ=255)                                                                          aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                             <  3:132672/119‑1 (MQ=255)                                                                          aCCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGAt                                                                                             <  3:127498/119‑1 (MQ=255)                                                                                   tcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGt                                                      >  4:203963/1‑149 (MQ=255)                                                                                     ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                                    >  5:356923/1‑149 (MQ=255)                                                                                     ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                                    >  6:132931/1‑149 (MQ=255)                                                                                     ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGAGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                                    >  1:269777/1‑149 (MQ=255)                                                                                     ccAGCAGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCa                                                         <  4:338272/144‑1 (MQ=255)                                                                                       aGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg‑cg                                                 <  6:356923/149‑1 (MQ=255)                                                                                           gCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                     <  1:353630/78‑1 (MQ=255)                                                                                           gCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                     >  2:353630/1‑78 (MQ=255)                                                                                                 tGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGc                                                                  <  2:33031/123‑1 (MQ=255)                                                                                                 tGAACAGCGCGCCGTTGAACAGACGCATCAAGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGc                                                                  >  1:33031/1‑123 (MQ=255)                                                                                                   aaCAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCtcgtc                                     >  7:42097/1‑149 (MQ=255)                                                                                                      aGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAg                                  <  2:269777/149‑1 (MQ=255)                                                                                                       gcgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAgg                                 >  7:27906/1‑149 (MQ=255)                                                                                                       gcgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAgg                                 <  1:147152/149‑1 (MQ=255)                                                                                                        cgcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGt                                >  2:208990/1‑149 (MQ=255)                                                                                                         gcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTAcg                                             <  1:235030/135‑1 (MQ=255)                                                                                                         gcgcCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTAcg                                             >  2:235030/1‑135 (MQ=255)                                                                                                                  aaCAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACt                      <  5:132931/149‑1 (MQ=255)                                                                                                                     aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTTACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGaa                   <  4:172185/149‑1 (MQ=255)                                                                                                                     aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGa                    >  7:118522/1‑148 (MQ=255)                                                                                                                       tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                                >  7:152595/2‑87 (MQ=37)                                                                                                                       tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                                >  7:157327/2‑87 (MQ=37)                                                                                                                       tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                                <  8:157327/86‑1 (MQ=37)                                                                                                                       tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCtt                                                                                <  8:152595/86‑1 (MQ=37)                                                                                                                         gCATCAGGCGGTCGTCGTCCTGCTGCGAGATGTCTTCGCTCTGGCGGGCATATCTGTTGTTGTCAGCTTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCa                                                         <  5:238492/108‑1 (MQ=255)                                                                                                                          cATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGcc              <  8:118522/149‑1 (MQ=255)                                                                                                                           aTCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCg             >  3:236654/1‑149 (MQ=255)                                                                                                                           aTCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCg             >  4:347598/1‑149 (MQ=255)                                                                                                                                 cGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACt        <  1:250581/149‑1 (MQ=255)                                                                                                                                      gACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAg                                  >  6:35793/1‑117 (MQ=255)                                                                                                                                      gACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAg                                  <  5:35793/117‑1 (MQ=255)                                                                                                                                      gACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCa  <  2:16105/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                   AGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCG‑CGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGGGCCA  >  NC_002947/805955‑806236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑