Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 896,592 G→C 6.2% V8V (GTG→GTC)  PP_0779 → methyl‑accepting chemotaxis transducer/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947896,5920GC 6.2% 206.4 / 4.3 65V8V (GTG→GTC) PP_0779methyl‑accepting chemotaxis transducer/sensory box protein
Reads supporting (aligned to +/- strand):  ref base G (34/27);  new base C (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

CTAATGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATGGTCAACGGCAATTTC  >  NC_002947/896444‑896739
                                                                                                                                                    |                                                                                                                                                   
cTAATGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTg                                                                                                                                                     <  7:332814/149‑1 (MQ=255)
   aTGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGa                                                                                                                                                    <  6:266403/147‑1 (MQ=255)
   aTGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGAt                                                                                                                                                   <  1:104351/148‑1 (MQ=255)
   aTGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATg                                                                                                                                                  <  4:270054/149‑1 (MQ=255)
   aTGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATg                                                                                                                                                  >  3:219113/1‑149 (MQ=255)
        aCCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTc                                                                                                                                               <  1:171504/147‑1 (MQ=255)
        aCCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTc                                                                                                                                               >  2:171504/1‑147 (MQ=255)
            ccTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTcaacaac                                                                                                                                         <  7:236811/149‑1 (MQ=255)
                gtgACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAg                                                                                                                                      <  4:275214/148‑1 (MQ=255)
                 tgACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGc                                                                                                                                     <  2:74496/148‑1 (MQ=255)
                    cGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTcaa                                                                                                                                 <  4:219113/149‑1 (MQ=255)
                     gTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTcaag                                                                                                                                >  1:4811/1‑149 (MQ=255)
                                 ccTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCgg                                                                                                                    <  1:201976/149‑1 (MQ=255)
                                         cgggCATTTTTGGCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCGGGAATCCGATGAAGATGCATTCGACTACGTGATGGGCTCGAGGAGAGCTGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGGAACTga                                                                                                             <  4:314038/146‑2 (MQ=255)
                                          cGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCATCCGATGAAGATTCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcgc                                                                                                           <  4:248456/149‑1 (MQ=255)
                                          cGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcgc                                                                                                           >  2:139271/1‑149 (MQ=255)
                                                       tttATGAATATTTCCTCACACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGt                                                                                              >  6:300836/1‑149 (MQ=255)
                                                            gAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAg                                                                                                       <  6:276955/135‑1 (MQ=255)
                                                            gAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAg                                                                                                       >  5:276955/1‑135 (MQ=255)
                                                            gAATACTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGt                                                                                              <  6:43713/144‑1 (MQ=255)
                                                            gAATACTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGt                                                                                              >  5:43713/1‑144 (MQ=255)
                                                               tatTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGaa                                                                                      <  1:281943/149‑1 (MQ=255)
                                                                      tCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGCAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGt                                                                                              >  2:534/1‑134 (MQ=255)
                                                                      tCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCAGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGt                                                                                              <  1:534/134‑1 (MQ=255)
                                                                               aCTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGtc                                                                      <  7:185454/149‑1 (MQ=255)
                                                                                cTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGtct                                                                     >  1:310919/1‑149 (MQ=255)
                                                                                 tCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGtctc                                                                    >  2:208529/1‑149 (MQ=255)
                                                                                   aagaagGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGa                                                                  >  5:185577/1‑149 (MQ=255)
                                                                                          tgtAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGc                                                              >  4:348760/1‑146 (MQ=255)
                                                                                            tAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGAt                                                         >  4:206195/1‑149 (MQ=255)
                                                                                                  gCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGt                                                   <  1:208529/149‑1 (MQ=255)
                                                                                                       cGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGtgctgc                                              <  5:300836/149‑1 (MQ=255)
                                                                                                        gATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCaa                                                                              >  2:104512/1‑116 (MQ=255)
                                                                                                        gATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCaa                                                                              <  1:104512/116‑1 (MQ=255)
                                                                                                        gATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGtgctg                                               >  8:170043/1‑147 (MQ=255)
                                                                                                          tGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGc                                           >  5:62968/1‑149 (MQ=255)
                                                                                                              gATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGAAGCTCGa                                       >  5:221528/1‑149 (MQ=255)
                                                                                                                 gCATTCGACTACGTGATGTGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGAccc                                    >  5:287528/1‑149 (MQ=255)
                                                                                                                   aTTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGt                                                                                 >  4:83868/1‑102 (MQ=255)
                                                                                                                   aTTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGt                                                                                 <  3:83868/102‑1 (MQ=255)
                                                                                                                   aTTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCt                                   >  2:241551/1‑148 (MQ=255)
                                                                                                                       gACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTg                                                     >  4:85530/1‑126 (MQ=255)
                                                                                                                       gACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTg                                                     <  3:85530/126‑1 (MQ=255)
                                                                                                                       gACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCa                                             <  5:257391/134‑1 (MQ=255)
                                                                                                                       gACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCa                                             >  6:257391/1‑134 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAaga                                                                            <  5:275217/102‑1 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAaga                                                                            >  6:275217/1‑102 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTCATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcg                                                                                                            <  4:125028/70‑1 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTCATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcg                                                                                                            <  4:119935/70‑1 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTCATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcg                                                                                                            >  3:119935/1‑70 (MQ=255)
                                                                                                                        aCTACGTGATGCGCTCCAGGAGAGCGGTCATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTgcg                                                                                                            >  3:125028/1‑70 (MQ=255)
                                                                                                                         cTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGAc                            <  6:62968/149‑1 (MQ=255)
                                                                                                                          tACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGTTCGACCCTCAGGGACg                           >  6:93321/1‑149 (MQ=255)
                                                                                                                            cGTGATGTGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGCCCCTCAGGGACGGa                         >  7:250291/1‑149 (MQ=255)
                                                                                                                             gTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGa                             >  2:229170/1‑144 (MQ=255)
                                                                                                                             gTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGa                             <  1:229170/144‑1 (MQ=255)
                                                                                                                             gTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGAt                        >  6:203830/1‑149 (MQ=255)
                                                                                                                                  gcgcTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATc                       >  1:357910/1‑145 (MQ=255)
                                                                                                                                     cTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATgg                >  5:138382/1‑149 (MQ=255)
                                                                                                                                     cTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATgg                >  5:144612/1‑149 (MQ=255)
                                                                                                                                       ccAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATGGt               >  1:48758/1‑148 (MQ=255)
                                                                                                                                           gagaGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCAATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGATCGACCCTCAGGGACGGATCGAGATGGTCAACg          >  1:11255/1‑149 (MQ=255)
                                                                                                                                                cGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAaga                                                                            <  6:166052/78‑1 (MQ=255)
                                                                                                                                                cGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAaga                                                                            >  5:166052/1‑78 (MQ=255)
                                                                                                                                                 ggTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATGGTCAACGGCAAtt    >  1:100089/1‑149 (MQ=255)
                                                                                                                                                   tGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCAAGATGGTCAACGGCAATTTc  <  5:203830/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTAATGTGACCGCCTTGTGACGTAAAGATGACGCCTGACTGCCGGCATTTTTGCCTTTATGAATATTTCCTCGCACAGCACTCAAGAAGGTGTAGCCGGCAGCCGATGAAGATGCATTCGACTACGTGATGCGCTCCAGGAGAGCGGTGATGTTCAACAACAAGCTCAAGCAAGAAATCCGGCAACTGCGCGAAGACCTGATGTCCATCGAACAGGTCAAGAGTAGTCTCGACAGCGAGATGCTGGTGCTGCAGCTCGACCCTCAGGGACGGATCGAGATGGTCAACGGCAATTTC  >  NC_002947/896444‑896739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑