BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,117	T→G	8.9%	N98H (AAC→CAC) ‡	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,117	0	T	G	8.9%	223.7 / 10.0	78	N98H (AAC→CAC) ‡	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand): ref base T (37/34); new base G (4/3); total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAG  >  NC_002947/99973‑100258

gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                           >  3:317342/1‑149 (MQ=255)

 cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                          <  6:234081/149‑1 (MQ=255)

  tCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         >  4:132917/1‑149 (MQ=255)

      ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                      >  3:125939/1‑148 (MQ=255)

       cTTGTTGGTGCGCGTGCGGTCCAGTTCACTGCAGTAGATCACCTTGAAGTGATGATCGATCGCAAAGTGCCGAGCGATTTCGAGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGCCGCa                                                                                                                                     <  8:240637/148‑1 (MQ=255)

       cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                      >  8:190848/1‑147 (MQ=255)

       cTTGTTGGTGCGCGTGACGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGCTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCACGTTCAGGGcagggg                                                                                                                                    >  6:182698/1‑144 (MQ=255)

          gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                  <  7:190848/148‑1 (MQ=255)

           ttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGTGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCa                                                                                                                                 <  1:211591/148‑1 (MQ=255)

              gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                             >  4:279406/1‑149 (MQ=255)

               tGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTcc                                                                                                                            <  2:291984/149‑1 (MQ=255)

                         gTCCTGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGAGTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                  <  7:334192/149‑1 (MQ=255)

                         gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                  <  8:183902/149‑1 (MQ=255)

                          tCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTc                                                                                                                 >  8:23439/1‑149 (MQ=255)

                                  aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                           >  1:195420/1‑147 (MQ=255)

                                   cTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGCCGAACTCCCATGGTTT‑GGAGGTCGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACATTCGAACACGAg                                                                                                        >  5:43996/1‑149 (MQ=255)

                                      ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                     >  5:56540/1‑149 (MQ=255)

                                         tAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                  <  5:153842/149‑1 (MQ=255)

                                              cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  2:211697/1‑147 (MQ=255)

                                              cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  1:204950/1‑147 (MQ=255)

                                               aCCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                             <  6:56540/148‑1 (MQ=255)

                                                 cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                          >  5:10548/1‑149 (MQ=255)

                                                 cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                          <  6:10548/149‑1 (MQ=255)

                                                  ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCt                                                                                                                   >  3:25286/1‑123 (MQ=255)

                                                  ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCt                                                                                                                   <  4:25286/123‑1 (MQ=255)

                                                     aaGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         >  7:34600/1‑98 (MQ=255)

                                                     aaGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                         <  8:34600/98‑1 (MQ=255)

                                                        tgatgaTAGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGACCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAc                                                                                   >  7:235655/1‑149 (MQ=255)

                                                         gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                         <  8:242099/126‑1 (MQ=255)

                                                         gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                         >  7:242099/1‑126 (MQ=255)

                                                          atgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgc                                                                                 <  1:172872/149‑1 (MQ=255)

                                                            gatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgcg                                                                                >  1:265536/1‑149 (MQ=255)

                                                              tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGa                                                                              <  4:125939/148‑1 (MQ=255)

                                                                 aaCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAGGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcg                                                                          >  2:69774/1‑149 (MQ=255)

                                                                     cAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               >  6:91844/1‑124 (MQ=255)

                                                                     cAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                               <  5:91844/124‑1 (MQ=255)

                                                                      aaaGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGTAATGTACACCGTGCGCCCCTCCCCGTTCAGGGCCGCAAGCAACTCCGCCACACCAACGAACACGAGTGTTACGTAAAGACCGGACCCACGGAAgcgaacgc                                                                     >  8:236763/1‑144 (MQ=255)

                                                                             cGAGCGATTTCGCGGGCGAACTCCCATGGGTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                              <  4:11707/149‑1 (MQ=255)

                                                                               aGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGt                                                            >  5:178309/1‑149 (MQ=255)

                                                                                    tttCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  3:279406/149‑1 (MQ=255)

                                                                                     ttCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCTCACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  3:132917/148‑1 (MQ=255)

                                                                                        gcccgCGAAATCGCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCg                                                                                        >  6:29689/5‑112 (MQ=255)

                                                                                            gCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATc                                                  >  8:261908/1‑146 (MQ=255)

                                                                                            gCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATc                                                  <  7:261908/146‑1 (MQ=255)

                                                                                              gAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                     >  1:128551/1‑125 (MQ=255)

                                                                                              gAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                     <  2:128551/125‑1 (MQ=255)

                                                                                               aaCTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGc                                            <  7:23439/149‑1 (MQ=255)

                                                                                                  tCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGTCtt                                         >  8:315567/1‑149 (MQ=255)

                                                                                                    ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                       <  1:69774/149‑1 (MQ=255)

                                                                                                    ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                       >  7:313455/1‑149 (MQ=255)

                                                                                                    ccATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                        <  2:195420/148‑1 (MQ=255)

                                                                                                     cATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCt                                          >  4:255630/1‑145 (MQ=255)

                                                                                                     cATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCt                                          <  3:255630/145‑1 (MQ=255)

                                                                                                       tGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTTAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCGCAGGCCTTGGCtt                                    >  3:43506/1‑149 (MQ=255)

                                                                                                                   tGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                         <  2:265536/149‑1 (MQ=255)

                                                                                                                     gCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGt                        <  8:235655/148‑1 (MQ=255)

                                                                                                                       aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       >  8:193100/1‑115 (MQ=255)

                                                                                                                       aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                       <  7:193100/115‑1 (MQ=255)

                                                                                                                       aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAg                      >  4:85878/1‑148 (MQ=255)

                                                                                                                          gTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                       >  3:149582/1‑144 (MQ=255)

                                                                                                                          gTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                       <  4:149582/144‑1 (MQ=255)

                                                                                                                             cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               <  6:178309/149‑1 (MQ=255)

                                                                                                                               gCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              >  7:160754/1‑148 (MQ=255)

                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               <  5:66619/141‑1 (MQ=255)

                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa               >  6:66619/5‑145 (MQ=255)

                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              <  1:151440/142‑1 (MQ=255)

                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt              >  2:151440/5‑146 (MQ=255)

                                                                                                                                 ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa            >  5:55325/5‑148 (MQ=255)

                                                                                                                                  tGCGGCCCTGCCAGTTCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa          <  7:236763/149‑1 (MQ=255)

                                                                                                                                    cGGACCTTCACGTTCATGGCCTCAAGCAACTCCGGCACACCTTCGAACACGATGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc        <  4:43506/149‑1 (MQ=255)

                                                                                                                                     ggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGcc       >  8:122653/1‑149 (MQ=255)

                                                                                                                                        ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc    <  8:160754/149‑1 (MQ=255)

                                                                                                                                        ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg     >  7:238948/1‑148 (MQ=255)

                                                                                                                                        ccTGCCAGTTCATGGCCTCAATCAAATCCGTCACACCTTCGAACAATAGTTTTTAGTAAAGAACGGTCAAGCTTAAGATCTCGCTGTAGAAGTTCACCTCATCCAAGGCCTTGGCTTATTAGAAGCTGTAGAACTGCATGAAAgaatgc    <  4:317342/149‑6 (MQ=255)

                                                                                                                                           gCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag  >  6:74689/1‑148 (MQ=255)

                                                                                                                                           gCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcag  >  2:296892/1‑148 (MQ=255)

                                                                                                                                             ccGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgc                                                                                 >  7:92070/1‑67 (MQ=255)

                                                                                                                                             ccGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAcgc                                                                                 <  8:92070/67‑1 (MQ=255)

GCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTT‑GGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAG  >  NC_002947/99973‑100258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`