Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 924,320 C→G 10.0% A291A (GCG→GCC)  PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947924,3200CG10.0% 108.2 / 3.9 40A291A (GCG→GCC) PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (17/19);  new base G (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

GCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGC  >  NC_002947/924174‑924462
                                                                                                                                                  |                                                                                                                                              
gCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcgc                                                                                                                                              <  7:191769/149‑1 (MQ=255)
gCCAACCGTGACAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcgc                                                                                                                                              >  8:72755/1‑149 (MQ=255)
   aaCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcgcg                                                                                                                                             >  1:320303/1‑147 (MQ=255)
   aaCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTcgcg                                                                                                                                             <  2:320303/147‑1 (MQ=255)
      catGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCt                                                                                                                                        >  5:154724/3‑149 (MQ=255)
          ccAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGAc                                                                                                                                    <  3:337101/149‑1 (MQ=255)
          ccAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCTggc                                                                                                                                     <  1:16046/148‑2 (MQ=255)
           cAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACg                                                                                                                                   >  3:229449/1‑149 (MQ=255)
                     tgtgCAAGCAACCCGCGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGt                                                                                                                          <  8:178107/148‑1 (MQ=255)
                           agcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt                                                                                                                   <  7:72755/149‑1 (MQ=255)
                           agcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt                                                                                                                   >  5:30056/1‑149 (MQ=255)
                            gcaaCCCGGGCAGCACCATGGCCAGCTGCGCGACCACCGGGAAGAACACCAGGACGCCGCCGATCAGCCCGAACACGAACAGGACCAGCAGGAAGACCTGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATc                                                                                                                  <  6:5998/149‑1 (MQ=255)
                            gcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATc                                                                                                                  >  4:178302/1‑149 (MQ=255)
                                       atctccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACATCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTc                                                                                                       <  7:225864/145‑1 (MQ=255)
                                            cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGAAGTGATAAGGTGGCGGATCGACTCCAGcgcgagat                                                                                                  >  8:225864/1‑145 (MQ=255)
                                                gCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACt                                                                                               <  5:281096/148‑1 (MQ=255)
                                                 ccAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                             >  1:222533/1‑149 (MQ=255)
                                                   agcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                           <  5:356505/149‑1 (MQ=255)
                                                      agcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt                                                                                        <  6:30056/149‑1 (MQ=255)
                                                                   ggATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCCCCGCCGTGCTGGACGTGATAAGGTCGCGGATCGACTCCACCTCCCGCAACTGGGAGATGCACGAGCCGGTg                                                                           >  7:100235/1‑149 (MQ=255)
                                                                    gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGGCGCGGTGCTGGACGTGCTCAGGTAGCGGCTAGACTACAGCAAGCGCAACAGCGAGATGAACGAGCCGGTg                                                                           >  2:134274/1‑148 (MQ=255)
                                                                                caCGCCGCGGATCCGCCAGAACACGAACCGGAACAGCAGGCAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGGCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCa                                                              <  8:220802/148‑1 (MQ=255)
                                                                                     cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAATGAGCCGGTGGACTTGGGCCGCACCGAg                                                         >  5:361526/1‑149 (MQ=255)
                                                                                     cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAg                                                         >  6:353786/1‑149 (MQ=255)
                                                                                      gccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGt                                                        >  2:243637/1‑149 (MQ=255)
                                                                                       ccgATCAGCCACAACACTAACTGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGGCGCGGTGCTGGACGTGATCAGGGCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGtt                                                       <  8:100235/149‑1 (MQ=255)
                                                                                              gCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg                                                <  1:134274/149‑1 (MQ=255)
                                                                                               ccAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggc                                               <  6:154724/149‑1 (MQ=255)
                                                                                                        gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGCTCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCAt                                      >  7:104818/1‑149 (MQ=255)
                                                                                                        gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGCGTTGCGCAGGCGCAGGGcct                                      >  2:192497/1‑147 (MQ=255)
                                                                                                        gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCAt                                      >  3:226674/1‑149 (MQ=255)
                                                                                                                  agcagGAAGAACGGCAGGTCGGCCAGCACCGGCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc                            <  5:188349/149‑1 (MQ=255)
                                                                                                                        cagaaCGGCAGGTCGGCCAGCACCGGCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCATCTCGCTCAACTGCGAGATGAACGAGCCGGGGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTc                      <  6:287377/148‑1 (MQ=255)
                                                                                                                        aagaaCGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTc                      >  7:243635/1‑149 (MQ=255)
                                                                                                                            aCGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTc                  <  1:146778/149‑1 (MQ=255)
                                                                                                                                cAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAccc              <  8:104818/149‑1 (MQ=255)
                                                                                                                                      gtccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                        >  1:356493/3‑133 (MQ=255)
                                                                                                                                      gtccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                        <  2:356493/131‑1 (MQ=255)
                                                                                                                                      ggCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                       <  2:270642/134‑1 (MQ=255)
                                                                                                                                      ggCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                       >  1:270642/1‑134 (MQ=255)
                                                                                                                                        ccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg      >  8:134238/1‑149 (MQ=255)
                                                                                                                                            cACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgc  <  4:229449/149‑1 (MQ=255)
                                                                                                                                               cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCa                          <  7:164166/122‑1 (MQ=255)
                                                                                                                                               cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCa                          >  8:164166/1‑122 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGC  >  NC_002947/924174‑924462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑