Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	989,155	G→T	7.0%	C336F (TGC→TTC)	hisS →	histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	989,155	0	G	T	7.0%	271.4 / 9.0	86	C336F (TGC→TTC)	hisS	histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (39/40);  new base T (4/2);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

GCCGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGT  >  NC_002947/989008‑989301                                                                                                                                                    |                                                                                                                                                    gCCGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCTCCAGATCGACGTTTACCtgtg                                                                                                                                                     <  1:212835/148‑1 (MQ=255) gCCGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgc                                                                                                                                                    >  2:280277/1‑149 (MQ=255) gCCGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgc                                                                                                                                                    >  6:165497/1‑149 (MQ=255)  ccGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgcg                                                                                                                                                   >  3:138049/1‑149 (MQ=255)  ccGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgcg                                                                                                                                                   >  3:143316/1‑149 (MQ=255)   cGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGCCGTTTACCTGTgcgc                                                                                                                                                  >  1:142312/1‑149 (MQ=255)   cGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgcgc                                                                                                                                                  >  5:219983/1‑149 (MQ=255)         gacgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                            <  5:82819/149‑1 (MQ=255)          acgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGc                                                                                                                                           <  2:175846/149‑1 (MQ=255)           cgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGc                                                                                                                                           >  6:141678/1‑148 (MQ=255)            ggCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCg                                                                                                                                          >  2:254553/1‑148 (MQ=255)              ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGaa                                                                                                                                        >  5:365295/1‑148 (MQ=255)              ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                         <  8:296151/147‑1 (MQ=255)              ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                         >  7:296151/1‑147 (MQ=255)                  gTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGc                                                                                                                                   >  5:364921/1‑149 (MQ=255)                     gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                  >  3:89108/1‑147 (MQ=255)                     gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                  >  7:258605/1‑147 (MQ=255)                       aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                     >  4:116557/1‑142 (MQ=255)                       aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                     <  3:116557/142‑1 (MQ=255)                         aGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgc                                                                                                                                                    >  5:39030/1‑125 (MQ=255)                         aGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTgc                                                                                                                                                    <  6:39030/125‑1 (MQ=255)                          gATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑ggg                                                                                                                          >  7:295946/1‑149 (MQ=255)                           aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                  >  1:230613/1‑141 (MQ=255)                           aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                  <  2:230613/141‑1 (MQ=255)                           aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggc                                                                                                                         >  8:111655/1‑149 (MQ=255)                              ggTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccg                                                                                                                       <  6:364921/148‑1 (MQ=255)                                tGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccggcc                                                                                                                    >  7:130904/1‑149 (MQ=255)                                   cAAGCCGACCACTGGCGTTGGTTTCGCCATTGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgc                                                                                                                 <  5:357944/149‑1 (MQ=255)                                    aaGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgcg                                                                                                                >  1:180568/1‑149 (MQ=255)                                          aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCttt                                                                                                                                              >  2:25653/1‑114 (MQ=255)                                          aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCttt                                                                                                                                              <  1:25653/114‑1 (MQ=255)                                          aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTc                                                                                                          <  8:258605/149‑1 (MQ=255)                                           ccACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTcc                                                                                                         <  5:165497/149‑1 (MQ=255)                                              cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                           >  4:213892/1‑144 (MQ=255)                                              cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                           <  3:213892/144‑1 (MQ=255)                                                 gCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAgg                                                                                                    <  6:45498/148‑1 (MQ=255)                                                    ttGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTg                                                                                                >  8:23235/1‑149 (MQ=255)                                                       gTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTgcgc                                                                                             <  1:280277/149‑1 (MQ=255)                                                        tttCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGACCAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGACGGCTTgcgct                                                                                            >  3:100615/1‑148 (MQ=255)                                                            gCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCg                                                                                        <  2:180568/149‑1 (MQ=255)                                                               aTGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                     >  1:37560/1‑149 (MQ=255)                                                                 gggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGc                                                                                   >  4:15728/1‑149 (MQ=255)                                                                  ggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGcc                                                                                  >  2:282890/1‑149 (MQ=255)                                                                   gCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCg                                                                                 >  7:149890/1‑149 (MQ=255)                                                                    cATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCgg                                                                                >  7:103656/1‑149 (MQ=255)                                                                     aTCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCggg                                                                               >  3:153392/1‑149 (MQ=255)                                                                      tCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                              <  5:141678/149‑1 (MQ=255)                                                                        gAGCGCCTGATCCTGCTGTTGGAAACCCTGGTCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGAGCCTTTGGCGAACAGGCGGAGC‑TGGCCTGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCt                                                                            >  1:55560/1‑149 (MQ=255)                                                                        gAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCt                                                                            <  4:89108/149‑1 (MQ=255)                                                                         aGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                            >  5:163137/1‑85 (MQ=255)                                                                         aGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                            <  6:163137/85‑1 (MQ=255)                                                                            gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                     >  8:166918/1‑136 (MQ=255)                                                                            gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                     <  7:166918/136‑1 (MQ=255)                                                                            gcCTGATCCTGCTGGTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTgcgc                                                                        <  6:273021/149‑1 (MQ=255)                                                                                gATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  7:111655/149‑1 (MQ=255)                                                                                 aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  4:143316/148‑1 (MQ=255)                                                                                 aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  4:138049/148‑1 (MQ=255)                                                                                  tCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  1:282890/148‑1 (MQ=255)                                                                                       ctgTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTc                                                               <  2:37560/147‑1 (MQ=255)                                                                                           tGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGc                                                          <  8:130904/148‑1 (MQ=255)                                                                                               aaCCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggtg                                                     <  6:365295/149‑1 (MQ=255)                                                                                                 cccTGGGCAAGGGGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGTAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggtggt                                                   <  6:152728/149‑1 (MQ=255)                                                                                                  ccTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGgtggtg                                                  <  7:23235/149‑1 (MQ=255)                                                                                                   cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  2:327089/130‑1 (MQ=255)                                                                                                   cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    >  1:327089/1‑130 (MQ=255)                                                                                                    tGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGtggtgg                                                 >  6:200407/1‑148 (MQ=255)                                                                                                      ggCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCa                                               <  3:15728/148‑1 (MQ=255)                                                                                                       gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                             >  8:323465/1‑149 (MQ=255)                                                                                                       gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                             >  8:98273/1‑149 (MQ=255)                                                                                                         aaGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑GGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCtt                                           <  8:295946/149‑1 (MQ=255)                                                                                                            gTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTAGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCaa                                        <  1:254553/149‑1 (MQ=255)                                                                                                                  gAGTCCCTCAGCCCCCAGATCGACGTTTACCTGTCCGCCTTTGCCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGCAGGCTTGCGCGACCGCATGCCGGGCCTGCGCCTGCCAGTCAACGCCGGTGGCGGCAGCTTCAAGAGCCa                                  <  8:149890/149‑1 (MQ=255)                                                                                                                    gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                      >  1:35654/1‑111 (MQ=255)                                                                                                                    gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                      <  2:35654/111‑1 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                              <  8:127468/100‑1 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                              >  7:127468/1‑100 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                      >  1:159943/1‑108 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                      <  2:159943/108‑1 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                             <  7:323465/149‑1 (MQ=255)                                                                                                                       cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                             <  7:98273/149‑1 (MQ=255)                                                                                                                            gccgccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGa                          >  5:15585/1‑147 (MQ=255)                                                                                                                               gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                     <  6:15585/149‑1 (MQ=255)                                                                                                                               gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                     >  2:217350/1‑149 (MQ=255)                                                                                                                                ccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                     >  1:162614/1‑148 (MQ=255)                                                                                                                                        agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                     >  5:65246/8‑140 (MQ=255)                                                                                                                                        agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                     <  6:65246/133‑1 (MQ=255)                                                                                                                                        aCGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGc            >  1:215947/1‑149 (MQ=255)                                                                                                                                           tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCTACGCCGGTGGTGGCAGCTTCAag                                       >  1:293797/1‑119 (MQ=255)                                                                                                                                           tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAag                                       <  2:293797/119‑1 (MQ=255)                                                                                                                                            ccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTg        >  2:112067/4‑149 (MQ=255)                                                                                                                                              aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                   >  2:326723/1‑136 (MQ=255)                                                                                                                                              aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                   <  1:326723/136‑1 (MQ=255)                                                                                                                                               ccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCAc     >  5:209857/1‑149 (MQ=255)                                                                                                                                                cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    >  5:160388/1‑85 (MQ=255)                                                                                                                                                cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                    <  6:160388/85‑1 (MQ=255)                                                                                                                                                  gtgCGCCTTTGGCGAACAGGCGGAGC‑TGGCCCGCCTGCGCCAGTCCGAAGGCTTGCGCGACCGCTAGCCGGGCCTGCGCCTGGCAGTCACCCCGGGTGGTGGCAGCTTCACGAGCCAGTTCAAGACCGCCGACAAGAGCTGTGCACTGt  >  8:149412/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCCGTTACGACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGT  >  NC_002947/989008‑989301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑