Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	989,161	T→A	7.0%	F338Y (TTT→TAT) ‡	hisS →	histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	989,161	0	T	A	7.0%	262.2 / 7.9	86	F338Y (TTT→TAT) ‡	hisS	histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (40/40);  new base A (4/2);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCC  >  NC_002947/989016‑989307                                                                                                                                                  |                                                                                                                                                    gacgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                                  <  5:82819/149‑1 (MQ=255)  acgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGc                                                                                                                                                 <  2:175846/149‑1 (MQ=255)   cgGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGc                                                                                                                                                 >  6:141678/1‑148 (MQ=255)    ggCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCg                                                                                                                                                >  2:254553/1‑148 (MQ=255)      ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGaa                                                                                                                                              >  5:365295/1‑148 (MQ=255)      ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                               >  7:296151/1‑147 (MQ=255)      ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                               <  8:296151/147‑1 (MQ=255)          gTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGc                                                                                                                                         >  5:364921/1‑149 (MQ=255)             gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                        >  3:89108/1‑147 (MQ=255)             gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                        >  7:258605/1‑147 (MQ=255)               aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                           >  4:116557/1‑142 (MQ=255)               aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                           <  3:116557/142‑1 (MQ=255)                  gATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑ggg                                                                                                                                >  7:295946/1‑149 (MQ=255)                   aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                        >  1:230613/1‑141 (MQ=255)                   aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                        <  2:230613/141‑1 (MQ=255)                   aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggc                                                                                                                               >  8:111655/1‑149 (MQ=255)                      ggTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccg                                                                                                                             <  6:364921/148‑1 (MQ=255)                        tGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccggcc                                                                                                                          >  7:130904/1‑149 (MQ=255)                           cAAGCCGACCACTGGCGTTGGTTTCGCCATTGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgc                                                                                                                       <  5:357944/149‑1 (MQ=255)                            aaGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgcg                                                                                                                      >  1:180568/1‑149 (MQ=255)                                  aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCttt                                                                                                                                                    <  1:25653/114‑1 (MQ=255)                                  aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCttt                                                                                                                                                    >  2:25653/1‑114 (MQ=255)                                  aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTc                                                                                                                <  8:258605/149‑1 (MQ=255)                                   ccACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTcc                                                                                                               <  5:165497/149‑1 (MQ=255)                                      cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                                 >  4:213892/1‑144 (MQ=255)                                      cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                                 <  3:213892/144‑1 (MQ=255)                                         gCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAgg                                                                                                          <  6:45498/148‑1 (MQ=255)                                            ttGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTg                                                                                                      >  8:23235/1‑149 (MQ=255)                                               gTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTgcgc                                                                                                   <  1:280277/149‑1 (MQ=255)                                                tttCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGACCAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGACGGCTTgcgct                                                                                                  >  3:100615/1‑148 (MQ=255)                                                    gCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCg                                                                                              <  2:180568/149‑1 (MQ=255)                                                       aTGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                           >  1:37560/1‑149 (MQ=255)                                                         gggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGc                                                                                         >  4:15728/1‑149 (MQ=255)                                                          ggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGcc                                                                                        >  2:282890/1‑149 (MQ=255)                                                           gCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCg                                                                                       >  7:149890/1‑149 (MQ=255)                                                            cATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCgg                                                                                      >  7:103656/1‑149 (MQ=255)                                                             aTCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCggg                                                                                     >  3:153392/1‑149 (MQ=255)                                                              tCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                                    <  5:141678/149‑1 (MQ=255)                                                                gAGCGCCTGATCCTGCTGTTGGAAACCCTGGTCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGAGCCTTTGGCGAACAGGCGGAGC‑TGGCCTGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCt                                                                                  >  1:55560/1‑149 (MQ=255)                                                                gAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCt                                                                                  <  4:89108/149‑1 (MQ=255)                                                                 aGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                                  <  6:163137/85‑1 (MQ=255)                                                                 aGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTgg                                                                                                                                                  >  5:163137/1‑85 (MQ=255)                                                                    gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                           <  7:166918/136‑1 (MQ=255)                                                                    gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                           >  8:166918/1‑136 (MQ=255)                                                                    gcCTGATCCTGCTGGTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTgcgc                                                                              <  6:273021/149‑1 (MQ=255)                                                                        gATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  7:111655/149‑1 (MQ=255)                                                                         aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  4:143316/148‑1 (MQ=255)                                                                         aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  4:138049/148‑1 (MQ=255)                                                                          tCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  1:282890/148‑1 (MQ=255)                                                                               ctgTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTc                                                                     <  2:37560/147‑1 (MQ=255)                                                                                   tGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGc                                                                <  8:130904/148‑1 (MQ=255)                                                                                       aaCCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggtg                                                           <  6:365295/149‑1 (MQ=255)                                                                                         cccTGGGCAAGGGGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGTAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCggtggt                                                         <  6:152728/149‑1 (MQ=255)                                                                                          ccTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGgtggtg                                                        <  7:23235/149‑1 (MQ=255)                                                                                           cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  2:327089/130‑1 (MQ=255)                                                                                           cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          >  1:327089/1‑130 (MQ=255)                                                                                            tGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGtggtgg                                                       >  6:200407/1‑148 (MQ=255)                                                                                              ggCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCa                                                     <  3:15728/148‑1 (MQ=255)                                                                                               gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                                   >  8:98273/1‑149 (MQ=255)                                                                                               gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                                   >  8:323465/1‑149 (MQ=255)                                                                                                 aaGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑GGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCtt                                                 <  8:295946/149‑1 (MQ=255)                                                                                                    gTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTAGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCaa                                              <  1:254553/149‑1 (MQ=255)                                                                                                          gAGTCCCTCAGCCCCCAGATCGACGTTTACCTGTCCGCCTTTGCCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGCAGGCTTGCGCGACCGCATGCCGGGCCTGCGCCTGCCAGTCAACGCCGGTGGCGGCAGCTTCAAGAGCCa                                        <  8:149890/149‑1 (MQ=255)                                                                                                            gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                            >  1:35654/1‑111 (MQ=255)                                                                                                            gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                            <  2:35654/111‑1 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                                    <  8:127468/100‑1 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGc                                                                                    >  7:127468/1‑100 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                            >  1:159943/1‑108 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCt                                                                            <  2:159943/108‑1 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                                   <  7:98273/149‑1 (MQ=255)                                                                                                               cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                                   <  7:323465/149‑1 (MQ=255)                                                                                                                    gccgccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGa                                >  5:15585/1‑147 (MQ=255)                                                                                                                       gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                           <  6:15585/149‑1 (MQ=255)                                                                                                                       gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                           >  2:217350/1‑149 (MQ=255)                                                                                                                        ccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                           >  1:162614/1‑148 (MQ=255)                                                                                                                                agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                           <  6:65246/133‑1 (MQ=255)                                                                                                                                agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                           >  5:65246/8‑140 (MQ=255)                                                                                                                                aCGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGc                  >  1:215947/1‑149 (MQ=255)                                                                                                                                   tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCTACGCCGGTGGTGGCAGCTTCAag                                             >  1:293797/1‑119 (MQ=255)                                                                                                                                   tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAag                                             <  2:293797/119‑1 (MQ=255)                                                                                                                                    ccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTg              >  2:112067/4‑149 (MQ=255)                                                                                                                                      aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                         >  2:326723/1‑136 (MQ=255)                                                                                                                                      aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                         <  1:326723/136‑1 (MQ=255)                                                                                                                                       ccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCAc           >  5:209857/1‑149 (MQ=255)                                                                                                                                        cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          <  6:160388/85‑1 (MQ=255)                                                                                                                                        cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTgg                                                                          >  5:160388/1‑85 (MQ=255)                                                                                                                                          gtgCGCCTTTGGCGAACAGGCGGAGC‑TGGCCCGCCTGCGCCAGTCCGAAGGCTTGCGCGACCGCTAGCCGGGCCTGCGCCTGGCAGTCACCCCGGGTGGTGGCAGCTTCACGAGCCAGTTCAAGACCGCCGACAAGAGCTGTGCACTGt        >  8:149412/1‑149 (MQ=255)                                                                                                                                                cTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGccc  >  3:170627/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GACGGCCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCGGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCC  >  NC_002947/989016‑989307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑