Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	989,167	A→C	7.3%	E340A (GAA→GCA)	hisS →	histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	989,167	0	A	C	7.3%	255.0 / 9.9	82	E340A (GAA→GCA)	hisS	histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (38/38);  new base C (4/2);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

CCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCTC  >  NC_002947/989021‑989315                                                                                                                                                   |                                                                                                                                                       ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGaa                                                                                                                                                       >  5:365295/1‑148 (MQ=255) ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                                        >  7:296151/1‑147 (MQ=255) ccTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGa                                                                                                                                                        <  8:296151/147‑1 (MQ=255)     gTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGc                                                                                                                                                  >  5:364921/1‑149 (MQ=255)        gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                                 >  3:89108/1‑147 (MQ=255)        gAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                                 >  7:258605/1‑147 (MQ=255)          aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                                    >  4:116557/1‑142 (MQ=255)          aCAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTTCGCCAAAGGCGCACAg                                                                                                                                                    <  3:116557/142‑1 (MQ=255)             gATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑ggg                                                                                                                                         >  7:295946/1‑149 (MQ=255)              aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                                 >  1:230613/1‑141 (MQ=255)              aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCg                                                                                                                                                 <  2:230613/141‑1 (MQ=255)              aTGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggc                                                                                                                                        >  8:111655/1‑149 (MQ=255)                 ggTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccg                                                                                                                                      <  6:364921/148‑1 (MQ=255)                   tGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑Tggccggcc                                                                                                                                   >  7:130904/1‑149 (MQ=255)                      cAAGCCGACCACTGGCGTTGGTTTCGCCATTGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgc                                                                                                                                <  5:357944/149‑1 (MQ=255)                       aaGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTgcg                                                                                                                               >  1:180568/1‑149 (MQ=255)                             aCCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTc                                                                                                                         <  8:258605/149‑1 (MQ=255)                              ccACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTcc                                                                                                                        <  5:165497/149‑1 (MQ=255)                                 cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                                          >  4:213892/1‑144 (MQ=255)                                 cTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGt                                                                                                                          <  3:213892/144‑1 (MQ=255)                                    gCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAgg                                                                                                                   <  6:45498/148‑1 (MQ=255)                                       ttGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTg                                                                                                               >  8:23235/1‑149 (MQ=255)                                          gTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTgcgc                                                                                                            <  1:280277/149‑1 (MQ=255)                                           tttCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGACCAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGACGGCTTgcgct                                                                                                           >  3:100615/1‑148 (MQ=255)                                               gCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCg                                                                                                       <  2:180568/149‑1 (MQ=255)                                                  aTGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                                    >  1:37560/1‑149 (MQ=255)                                                    gggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGc                                                                                                  >  4:15728/1‑149 (MQ=255)                                                     ggCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGcc                                                                                                 >  2:282890/1‑149 (MQ=255)                                                      gCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑g                                                                                               >  7:149890/1‑149 (MQ=255)                                                       cATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑gg                                                                                              >  7:103656/1‑149 (MQ=255)                                                        aTCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑ggg                                                                                             >  3:153392/1‑149 (MQ=255)                                                         tCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGc                                                                                            <  5:141678/149‑1 (MQ=255)                                                           gAGCGCCTGATCCTGCTGTTGGAAACCCTGGTCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGAGCCTTTGGCGAACAGGCGGAGC‑TGGCCTGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCt                                                                                          >  1:55560/1‑149 (MQ=255)                                                           gAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCt                                                                                          <  4:89108/149‑1 (MQ=255)                                                               gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                                    >  8:166918/1‑136 (MQ=255)                                                               gcCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCtt                                                                                                    <  7:166918/136‑1 (MQ=255)                                                               gcCTGATCCTGCTGGTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTgcgc                                                                                      <  6:273021/149‑1 (MQ=255)                                                                   gATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  7:111655/149‑1 (MQ=255)                                                                    aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  4:143316/148‑1 (MQ=255)                                                                    aTCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  4:138049/148‑1 (MQ=255)                                                                     tCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGCTTGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  1:282890/148‑1 (MQ=255)                                                                          ctgTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTc                                                                             <  2:37560/147‑1 (MQ=255)                                                                              tGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGc                                                                        <  8:130904/148‑1 (MQ=255)                                                                                  aaCCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCggtg                                                                   <  6:365295/149‑1 (MQ=255)                                                                                    cccTGGGCAAGGGGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGTAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCggtggt                                                                 <  6:152728/149‑1 (MQ=255)                                                                                     ccTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGgtggtg                                                                <  7:23235/149‑1 (MQ=255)                                                                                      cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  >  1:327089/1‑130 (MQ=255)                                                                                      cTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  2:327089/130‑1 (MQ=255)                                                                                       tGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGtggtgg                                                               >  6:200407/1‑148 (MQ=255)                                                                                         ggCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCa                                                             <  3:15728/148‑1 (MQ=255)                                                                                          gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                                           >  8:323465/1‑149 (MQ=255)                                                                                          gCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGc                                                           >  8:98273/1‑149 (MQ=255)                                                                                            aaGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑GGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCtt                                                         <  8:295946/149‑1 (MQ=255)                                                                                               gTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTAGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCaa                                                      <  1:254553/149‑1 (MQ=255)                                                                                                     gAGTCCCTCAGCCCCCAGATCGACGTTTACCTGTCCGCCTTTGCCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGCAGGCTTGCGCGACCGCATGCC‑GGGCCTGCGCCTGCCAGTCAACGCCGGTGGCGGCAGCTTCAAGAGCCa                                                <  8:149890/149‑1 (MQ=255)                                                                                                       gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCt                                                                                    >  1:35654/1‑111 (MQ=255)                                                                                                       gTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCt                                                                                    <  2:35654/111‑1 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGc                                                                                            <  8:127468/100‑1 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGc                                                                                            >  7:127468/1‑100 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCt                                                                                    <  2:159943/108‑1 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCt                                                                                    >  1:159943/1‑108 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                                           <  7:98273/149‑1 (MQ=255)                                                                                                          cATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCa                                           <  7:323465/149‑1 (MQ=255)                                                                                                               gccgccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGa                                        >  5:15585/1‑147 (MQ=255)                                                                                                                  gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                                   <  6:15585/149‑1 (MQ=255)                                                                                                                  gccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                                   >  2:217350/1‑149 (MQ=255)                                                                                                                   ccAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                                   >  1:162614/1‑148 (MQ=255)                                                                                                                           agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                                   <  6:65246/133‑1 (MQ=255)                                                                                                                           agctccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGcc                                   >  5:65246/8‑140 (MQ=255)                                                                                                                           aCGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGc                          >  1:215947/1‑149 (MQ=255)                                                                                                                              tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCTACGCCGGTGGTGGCAGCTTCAag                                                     >  1:293797/1‑119 (MQ=255)                                                                                                                              tttACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAag                                                     <  2:293797/119‑1 (MQ=255)                                                                                                                               ccgccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTg                      >  2:112067/4‑149 (MQ=255)                                                                                                                                 aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                                 <  1:326723/136‑1 (MQ=255)                                                                                                                                 aCCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGa                                 >  2:326723/1‑136 (MQ=255)                                                                                                                                  ccTGTTCGCCAAAGGCGCACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCAc                   >  5:209857/1‑149 (MQ=255)                                                                                                                                   cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  <  6:160388/85‑1 (MQ=255)                                                                                                                                   cTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTgg                                                                                  >  5:160388/1‑85 (MQ=255)                                                                                                                                     gtgCGCCTTTGGCGAACAGGCGGAGC‑TGGCCCGCCTGCGCCAGTCCGAAGGCTTGCGCGACCGCTAGCC‑GGGCCTGCGCCTGGCAGTCACCCCGGGTGGTGGCAGCTTCACGAGCCAGTTCAAGACCGCCGACAAGAGCTGTGCACTGt                >  8:149412/1‑149 (MQ=255)                                                                                                                                           cTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGccc          >  3:170627/1‑149 (MQ=255)                                                                                                                                              tGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTg        <  4:153392/148‑1 (MQ=255)                                                                                                                                                gCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCCTTGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAACAGCGGTGCCCTGTTCGCCCTGAt      >  1:283771/1‑149 (MQ=255)                                                                                                                                                gCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGaaa                                      <  3:99102/116‑1 (MQ=255)                                                                                                                                                gCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGaaa                                      >  4:99102/1‑116 (MQ=255)                                                                                                                                                gCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATc     >  5:261895/1‑149 (MQ=255)                                                                                                                                                   aaCAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCTc  <  5:200407/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CCTGGTCGAACAGATGGGTGGCAAGCCGACCACTGGCGTTGGTTTCGCCATGGGCATCGAGCGCCTGATCCTGCTGTTGGAAACCCTGGGCAAGGTGCCGGAGTCCATCAGCCGCCAGATCGACGTTTACCTGTGCGCCTTTGGCGAACAGGCGGAGC‑TGGCCGGCCTGCGCCTGTCCGAAGGCTTGCGCGACCGCTTGCC‑GGGCCTGCGCCTGGCAGTCAACGCCGGTGGTGGCAGCTTCAAGAGCCAGTTCAAGAAAGCCGACAAGAGCGGTGCACTGTTCGCCCTGATCCTC  >  NC_002947/989021‑989315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑