Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	994,947	T→A	14.2%	intergenic (+26/+99)	PP_0859 → / ← PP_0860	ketoglutaramate omega‑amidase/sulfite reductase flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	994,947	0	T	A	14.2%	153.7 / 12.9	62	intergenic (+26/+99)	PP_0859/PP_0860	ketoglutaramate omega‑amidase/sulfite reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (25/28);  new base A (4/5);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTCAGTGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTCGATC  >  NC_002947/994802‑995082                                                                                                                                                   |                                                                                                                                        cTGCTCAGTGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGc                                                                                                                                      <  7:53923/149‑1 (MQ=255)   gCTCAGTGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTCCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGccc                                                                                                                                    <  7:147021/149‑1 (MQ=255)     tCAGTGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCcgc                                                                                                                                  >  7:72534/1‑149 (MQ=255)        gTGCGGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGCGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCAAGCCCGCGaa                                                                                                                               <  2:334933/149‑1 (MQ=255)         tGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGa                                                                                                                                >  1:342331/1‑147 (MQ=255)           ccGGGGAGGCGGACGGGGTGGTCACTGCAGAACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGCTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCAAGCCCGCGaagaa                                                                                                                            <  3:37834/149‑4 (MQ=255)             ggggAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGggc                                                                                                                          <  1:96318/149‑1 (MQ=255)                   gCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGTGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGccc                                                                                                                    >  1:79983/1‑149 (MQ=255)                   gCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGccc                                                                                                                    >  5:319182/1‑149 (MQ=255)                       aCGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGGCCGGCCCTTTCGCGGGCAAGCCCGCGAAGAAGCCGGGCCTgct                                                                                                                <  7:236675/149‑2 (MQ=37)                           ggTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGccc                                                                                                                                    <  6:115765/125‑1 (MQ=255)                           ggTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGccc                                                                                                                                    >  5:115765/1‑125 (MQ=255)                                 cACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCt                                                                                                                   >  7:130856/1‑136 (MQ=255)                                 cACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCt                                                                                                                   <  8:130856/136‑1 (MQ=255)                                  aCTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTcc                                                                                                     <  6:319182/149‑1 (MQ=255)                                  aCTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTcc                                                                                                     <  2:79983/149‑1 (MQ=255)                                    tGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAgg                                                                                                                            <  7:210987/124‑1 (MQ=255)                                    tGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAgg                                                                                                                            >  8:210987/1‑124 (MQ=255)                                    tGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCAc                                                                                                   >  2:173442/1‑149 (MQ=255)                                       aGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCgg                                                                                                <  3:304648/149‑1 (MQ=255)                                           cTGAGCGCGGCGGAACTGGCGGCGTACCGGGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCg                                                                                             >  1:209185/1‑149 (MQ=255)                                            tGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGtt                                                                                           >  2:122822/1‑149 (MQ=255)                                                                    aCC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGc                                                                                               <  1:348251/121‑1 (MQ=255)                                                                    aCC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCAGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTAt                                                                    >  8:193662/1‑148 (MQ=255)                                                                      c‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTcg                                                                 >  5:341226/1‑149 (MQ=255)                                                                        gggCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTcgc                                                                <  6:341226/149‑1 (MQ=255)                                                                         ggCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCAACCGTATTcgcg                                                               >  7:7688/1‑149 (MQ=255)                                                                          gCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCgg                                                              <  1:173442/149‑1 (MQ=255)                                                                                ttCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAc                                                        <  8:7688/149‑1 (MQ=255)                                                                                ttCCCGGACAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGCGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAc                                                        <  6:88742/149‑1 (MQ=255)                                                                                    cGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCAc                                                                                        <  7:224631/113‑1 (MQ=255)                                                                                    cGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCAc                                                                                        >  8:224631/1‑113 (MQ=255)                                                                                    cGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGc                                                    <  2:209185/149‑1 (MQ=255)                                                                                        cAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTcc                                                 >  4:242363/1‑148 (MQ=255)                                                                                         aaTCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTa                                               <  7:184793/149‑1 (MQ=255)                                                                                              ggATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacacac                                          >  3:85380/1‑149 (MQ=255)                                                                                               gATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTacacac                                          <  2:24096/148‑1 (MQ=255)                                                                                                aTGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAc                                                        >  8:319386/1‑133 (MQ=255)                                                                                                aTGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAAc                                                        <  7:319386/133‑1 (MQ=255)                                                                                                          cTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATacac                              <  2:342331/149‑1 (MQ=255)                                                                                                          cTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATacac                              <  8:114609/149‑1 (MQ=255)                                                                                                             cGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACAtc                           >  4:74723/1‑149 (MQ=255)                                                                                                             cGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACAt                            >  4:219957/1‑148 (MQ=255)                                                                                                                 cTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGc                       <  4:85380/149‑1 (MQ=255)                                                                                                                    cACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCg                                                     <  7:8890/116‑1 (MQ=255)                                                                                                                    cACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCg                                                     >  8:8890/1‑116 (MQ=255)                                                                                                                    cACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGa                    >  3:190289/1‑149 (MQ=255)                                                                                                                       tGACAGCAGGCCCGGCTTCTTCGCCGGCTAGCCCGCGAAACGGCCCGCCCTGCCACCAAATTTCCACCCGCCGATAACCCTTGCCCCCCCCACCGTATTCGCGGCAAAACCCGCTCCTACACACCCAACAATAACCATCACGCCCatat                 >  4:95016/1‑149 (MQ=255)                                                                                                                         aCAGCAGGCCCGGCTTCTTCGCGGGCTTGCGCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCg               >  1:29152/1‑149 (MQ=255)                                                                                                                          cagcagGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGc              <  7:193662/149‑1 (MQ=255)                                                                                                                                gCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCTCCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGcc                      <  1:275439/135‑1 (MQ=255)                                                                                                                                gCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGcc                      >  2:275439/1‑135 (MQ=255)                                                                                                                                 cccGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCt       <  3:219957/149‑1 (MQ=255)                                                                                                                                  ccGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTc      >  1:68010/1‑149 (MQ=255)                                                                                                                                   cggccctttcGCGGGCAAGCCCGCGAAGAAGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTc      >  2:145381/8‑148 (MQ=21)                                                                                                                                     gCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTCGAt   <  1:122822/149‑1 (MQ=255)                                                                                                                                      cttcttCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTCGATc  <  3:242363/149‑1 (MQ=255)                                                                                                                                       cctttcGCGGGCAAGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTCGATc  >  5:214406/4‑148 (MQ=255)                                                                                                                                         tttcGCGGGCAAGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGAt                                      <  2:207516/109‑1 (MQ=255)                                                                                                                                         tttcGCGGGCAAGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGAt                                      >  1:207516/2‑110 (MQ=255)                                                                                                                                         tttcGCGGGCAAGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAAt                                  >  6:145245/2‑114 (MQ=255)                                                                                                                                         tttcGCGGGCAAGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAAt                                  <  5:145245/113‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                        CTGCTCAGTGCCGGGGAGGCGGACGGGGTGTTCACTGCAGTACTGAGCGCGGCGGAACTGGCGGCGTACC‑GGGCCAAGTTCCCGGCCAATCTGGATGCCGATACCTTCGAGCTGCACTGACAGCAGGCCCGGCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGCCCTGCCAGCCAATTTCCACCGGCCGTTCACCGTTGCCGCCGCCACCGTATTCGCGGGTAAACCCGCTCCTACACACCGATCAATACACATCTCGCCGATATCGCCCATCCTCGATC  >  NC_002947/994802‑995082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑