| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,013,194 | T→A | 13.9% | intergenic (+22/+109) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,194 | 0 | T | A | 13.9% | 89.7 / 6.1 | 36 | intergenic (+22/+109) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (12/19); new base A (2/3); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GAAAACTTGGCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGA > NC_002947/1013050‑1013331 | gAAAACTTGGCCATCGACGGCGGCGGTCACCAGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAACAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAACGCCAGAAATCGGGGCTGCATTGCAg > 4:99356/1‑149 (MQ=255) aaaCTTGGCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGcc < 1:224065/149‑1 (MQ=255) aCTTGGCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCAAAGCAGcccc < 1:66320/149‑2 (MQ=255) ggCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTcg < 1:217821/148‑1 (MQ=255) ggCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCAAAGCAGccccga < 4:11547/147‑4 (MQ=255) ccATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCgg < 5:83055/149‑1 (MQ=255) ccATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCgg < 6:332926/149‑1 (MQ=255) ggcggTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGc < 2:354661/140‑1 (MQ=255) ggcggTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCaagatcgg > 1:354661/1‑141 (MQ=255) tGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGaa > 7:166987/1‑149 (MQ=255) tGACCTACCTGGCGCCGACCCGGATCAACCTGGCGCTGATGCAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCATCGCGGGCACGCCCGCTCCCACAggta > 1:65317/1‑147 (MQ=255) ctacctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCAc < 6:51493/149‑1 (MQ=255) acctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAc > 4:135694/1‑147 (MQ=255) cctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCa < 3:135694/148‑1 (MQ=255) ctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGa > 4:60381/1‑149 (MQ=255) tGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc > 5:124450/1‑149 (MQ=255) gcCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATGAAGTTCCGCGCTACCCGCGAGCACCATTAAGGGCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCtcc < 3:99356/149‑3 (MQ=38) gcCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTgg < 8:166987/149‑1 (MQ=255) ggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt < 6:20262/149‑1 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc < 7:87648/149‑1 (MQ=255) cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 4:352698/149‑1 (MQ=255) cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 6:124450/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAGGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGACATGTGATACCTGTGGGAGCGGGc < 7:52660/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGGCATGTGATACCTGTGGGAGCGGGc < 3:60381/149‑1 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGccc > 8:274982/1‑92 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGccc < 7:274982/92‑1 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGc > 1:53901/1‑146 (MQ=255) ttAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCACCCACAGGAATAACCAGACCAGGAGGGGAAGTGATACCGGTGGGAGCGGGCGGGGCCGCGACGGGGCGCGAGGGGGGCCCAATTGAATTTGCggggtt > 4:282149/1‑145 (MQ=19) ggCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt < 7:216367/88‑1 (MQ=255) ggCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt > 8:216367/1‑88 (MQ=255) ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGTGGTCATGTGATACCTGTGGGAGCGGGCCGGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCt > 1:34029/1‑141 (MQ=255) ccTGAAATCGGGGCTGCTTTCCAGCCCGTCGCGGGCACGCCCGCTCCCCCAGGAATCACCAGACCTGGAGGTCATGGGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCt < 2:34029/141‑1 (MQ=255) cgaagggCTGCAAAGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg > 1:332066/5‑81 (MQ=21) ggggCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCCACCGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa < 3:282149/148‑1 (MQ=255) ggggCCGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt > 2:110422/1‑66 (MQ=14) ggggCCGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 1:110422/66‑1 (MQ=14) | GAAAACTTGGCCATCGACGGCGGCGGTCACCTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGA > NC_002947/1013050‑1013331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |