Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,033,599 T→A 8.5% G73G (GGT→GGA)  PP_0891 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,033,5990TA 8.5% 164.9 / 4.1 59G73G (GGT→GGA) PP_0891hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base A (3/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GATGGCGAATCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACG  >  NC_002947/1033456‑1033743
                                                                                                                                               |                                                                                                                                                
gATGGCGAATCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTc                                                                                                                                              <  5:151195/148‑1 (MQ=255)
     cGAATCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGc                                                                                                                                        >  3:27332/1‑149 (MQ=255)
      gAATCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGAGCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCg                                                                                                                                       >  8:81037/1‑149 (MQ=255)
       aaTCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGt                                                                                                                                      >  8:196620/1‑149 (MQ=255)
       aaTCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGAATACCGCTGctc                                                                                                                                      <  4:63377/149‑3 (MQ=255)
             cACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGa                                                                                                                                 <  7:81037/148‑1 (MQ=255)
             cACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGa                                                                                                                                 <  2:264645/148‑1 (MQ=255)
              aCGGCTGTCGTTCATCCCCGGCAACAAAGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAg                                                                                                                                <  7:51651/148‑1 (MQ=255)
              aCGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGc                                                                                                                               <  6:99072/149‑1 (MQ=255)
                       gTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATgcgc                                                                                                                       <  7:44676/148‑1 (MQ=255)
                                  gCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTc                                                                                                           >  3:258762/1‑149 (MQ=255)
                                     aGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCCAATGCGCGACAACCTGTTCGAg                                                                                                        <  7:55382/149‑1 (MQ=255)
                                           gCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAg                                                                                                  >  1:222572/1‑149 (MQ=255)
                                               cacCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCt                                                                                              >  1:279529/1‑149 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAAc                                                                                                                 <  2:236032/127‑1 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAAc                                                                                                                 >  1:236032/1‑127 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGt                                                                                                      <  3:187041/138‑1 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGt                                                                                                      >  4:187041/1‑138 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGa                                                                                                   >  5:41774/1‑141 (MQ=255)
                                                  cgccgAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGa                                                                                                   <  6:41774/141‑1 (MQ=255)
                                                       aaGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAg                                                                                      <  2:222572/149‑1 (MQ=255)
                                                            cATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCca                                                                                  >  3:4855/1‑148 (MQ=255)
                                                             aTCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCTGTATTCCGCTGCGTGACGAGCAAATgc                                                                                                                         <  4:5422/108‑1 (MQ=255)
                                                             aTCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATgc                                                                                                                         >  3:5422/1‑108 (MQ=255)
                                                                gCTTTCTGTTGGTGCATGGCACGGTCGAGCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTg                                                                             <  4:4855/149‑1 (MQ=255)
                                                                    tCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGaa                                                                           <  3:65971/147‑1 (MQ=255)
                                                                        gtgtTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGca                                                                       >  6:6064/1‑147 (MQ=255)
                                                                             gCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATc                                                                 <  4:27332/148‑1 (MQ=255)
                                                                                  gCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTg                                                           <  7:196620/149‑1 (MQ=255)
                                                                                   cACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTg                                                           >  3:236907/1‑148 (MQ=255)
                                                                                          gATCGCAAGGGTAGGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGTACAGATCGACCTGCAGCCGa                                                    <  4:308639/148‑1 (MQ=255)
                                                                                           aTCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGCATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGAt                                                   >  4:32001/1‑148 (MQ=255)
                                                                                              gCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAAt                                               <  2:279529/149‑1 (MQ=255)
                                                                                              gCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGCATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAAt                                               <  3:32001/149‑1 (MQ=255)
                                                                                               cAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGcgcg                                                                                                                      >  3:309810/1‑77 (MQ=255)
                                                                                               cAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGcgcg                                                                                                                      <  4:309810/77‑1 (MQ=255)
                                                                                               cAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAAt                                               >  7:97197/1‑148 (MQ=255)
                                                                                                aaGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGa                                                                                       <  2:166980/107‑1 (MQ=255)
                                                                                                   ggTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCt                                          >  1:143478/1‑149 (MQ=255)
                                                                                                    gTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTg                                         <  4:258762/149‑1 (MQ=255)
                                                                                                    gTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTg                                         >  4:148327/1‑149 (MQ=255)
                                                                                                         gCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGa                                    >  2:94629/1‑149 (MQ=255)
                                                                                                         gCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGATCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTCCAGCCGATCAATGACCTGGCCGa                                    >  4:285403/1‑149 (MQ=255)
                                                                                                          ccGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGcaca                                                                     >  4:317257/1‑115 (MQ=255)
                                                                                                          ccGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGcaca                                                                     <  3:317257/115‑1 (MQ=255)
                                                                                                              gTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTg                               <  8:97197/149‑1 (MQ=255)
                                                                                                                         agaTGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGaa                    >  7:336447/1‑149 (MQ=255)
                                                                                                                          gaTGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGAc                                                              >  7:68901/1‑106 (MQ=255)
                                                                                                                          gaTGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGAc                                                              <  8:68901/106‑1 (MQ=255)
                                                                                                                               aCTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGcac                                                                      >  2:190130/1‑93 (MQ=255)
                                                                                                                               aCTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGcac                                                                      <  1:190130/93‑1 (MQ=255)
                                                                                                                                   gtcacgcagcGGAATACCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGc          >  3:6150/6‑149 (MQ=255)
                                                                                                                                   ggTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTg           <  1:94629/148‑1 (MQ=255)
                                                                                                                                    tcacgcagcGGAATACCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAgg                                                                         <  4:348235/81‑1 (MQ=255)
                                                                                                                                    tcacgcagcGGAATACCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAgg                                                                         >  3:348235/5‑85 (MQ=255)
                                                                                                                                    gTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGcc         >  1:152032/1‑149 (MQ=255)
                                                                                                                                     cacgcagcGGAATACCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCg        >  6:98554/4‑149 (MQ=255)
                                                                                                                                           gcGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACg  <  3:148327/149‑1 (MQ=255)
                                                                                                                                              gTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTgg                                        <  6:102363/108‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GATGGCGAATCTTCACGGCTGTCGTTCATCACCGGCAAGAACGGCGACACCGCCGAAGTGCATCGCTTTCTGGTGTTGCATGGCACGGTCGATCGCAAGGGTATGGCCGGGTTGAGGATCGAGATGGACTCGGTGAGCAGCGGTATTCCGCTGCGTGACGAGCAAATGCGCGACAACCTGTTCGAGGTCGAGCGCTTCGCCGAGGCCACAGTGAAGGCACAGATCGACCTGCAGCCGATCAATGACCTGGCCGACGGTGCACAGATCGAATTGCGCCTGCCGCTGACG  >  NC_002947/1033456‑1033743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑