Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,092,896 T→G 6.2% R268R (CGA→CGC)  PP_0949 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,092,8960TG 6.2% 189.1 / 3.1 65R268R (CGA→CGC) PP_0949ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (26/34);  new base G (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTACATATCTT  >  NC_002947/1092748‑1093038
                                                                                                                                                    |                                                                                                                                               
cggccACACCGACGAACTTGGCTGCCGCGCGGGCATGCAGACCCTGCTTGTTGATGATGGTGATTTGGCGGGCGGGCATCTGGGGCGGATCGTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGgtgt                                                                                                                                                 <  8:223582/149‑1 (MQ=255)
cggccACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGt                                                                                                                                                 <  4:321445/149‑1 (MQ=255)
   ccACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGa                                                                                                                                              <  1:276647/149‑1 (MQ=255)
   ccACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGa                                                                                                                                              <  7:61704/149‑1 (MQ=255)
    cacaCCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGAc                                                                                                                                             >  6:153258/1‑149 (MQ=255)
       acCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCaa                                                                                                                                          <  7:29456/149‑1 (MQ=255)
               acatGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGAAAAAGGCGCTCg                                                                                                                                  >  1:303398/4‑149 (MQ=255)
                 ttGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGt                                                                                                                                >  5:35542/1‑149 (MQ=255)
                 ttGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGt                                                                                                                                <  7:289059/149‑1 (MQ=255)
                   ggCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGGCGACCAAGGCGCTCGGTGa                                                                                                                              >  3:164778/1‑149 (MQ=255)
                   ggCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGGCGACCAAGGCGCTCGGTGa                                                                                                                              >  3:159344/1‑149 (MQ=255)
                              gggCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGaa                                                                                                                  <  3:95913/149‑1 (MQ=255)
                                   tGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGt                                                                                                              >  7:266265/1‑148 (MQ=255)
                                   tGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTg                                                                                                             <  1:119102/149‑1 (MQ=255)
                                    gCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTg                                                                                                             <  4:147809/148‑1 (MQ=255)
                                    gCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTg                                                                                                             >  3:147809/1‑148 (MQ=255)
                                                 gttgATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGGGATGGAGGACATGGACTTTTTTAAGGTTTTGCTGCAGATTACTACCAAGGCCCTCTGTGA‑TGTACACGCAACGGTGATGTccgccgttc                                                                                                >  7:129758/1‑145 (MQ=255)
                                                 gttgATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTg                                                                                                >  5:9885/1‑148 (MQ=255)
                                                     atgatgGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGcc                                                                                           >  7:134614/1‑149 (MQ=255)
                                                     atgatgGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGc                                                                                            <  1:193788/148‑1 (MQ=255)
                                                      tgatgGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTg                                                                                                                               <  6:167838/113‑1 (MQ=255)
                                                      tgatgGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTg                                                                                                                               >  5:167838/1‑113 (MQ=255)
                                                         tgGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATg                                                                                       <  3:72295/149‑1 (MQ=255)
                                                            tGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGc                                                                                     >  6:289632/1‑148 (MQ=255)
                                                               tttCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATg                                                                                 <  4:224056/149‑1 (MQ=255)
                                                                ttCGTGGGCGGGCTTCTTGGGCGTATCCTTGGGCTAGAGGTCGCGATGGCGGAGCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATg                                                                                 <  8:129758/148‑1 (MQ=255)
                                                                   gctggcgggcATCTGGGGCGTATCGTTGGGCTAGAGGTCGCGATGGCGGACCTGTACGTTTTTCAGGGTTTTCTGCAGGTGTCGACCAATTCGCTCGGTGA‑TGTACACCGAAGGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGtt                                                                              <  8:134614/145‑1 (MQ=255)
                                                                    cgggcgggCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTAc                                                                            <  5:289632/149‑1 (MQ=255)
                                                                           gcATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGgcg                                                                     >  6:360496/1‑149 (MQ=255)
                                                                                tGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGtt                                                                >  3:105500/1‑149 (MQ=255)
                                                                                  gggCGGATCCTTGGGGTAGAGGTCGCGATGGCGGACCTGGACGTTTTTAAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGc                                                              >  8:3868/1‑149 (MQ=255)
                                                                                    gCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTg                                                            >  6:41183/1‑149 (MQ=255)
                                                                                       gATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATg                                                                                       <  6:32131/119‑1 (MQ=255)
                                                                                       gATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATg                                                                                       >  5:32131/1‑119 (MQ=255)
                                                                                        aTCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGGACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAg                                                        <  3:18014/149‑1 (MQ=255)
                                                                                                gCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCgc                                                >  2:288291/1‑148 (MQ=255)
                                                                                                gCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAAAGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCgg                                                >  3:308734/1‑149 (MQ=255)
                                                                                                   agagGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGAt                                                                                        <  5:189673/106‑1 (MQ=255)
                                                                                                   agagGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGAt                                                                                        >  6:189673/1‑106 (MQ=255)
                                                                                                          gcgATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCAtt                                      <  6:9885/149‑1 (MQ=255)
                                                                                                          gcgATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGGCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCAtt                                      <  4:164778/149‑1 (MQ=255)
                                                                                                          gcgATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGGCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCAtt                                      <  4:159344/149‑1 (MQ=255)
                                                                                                           cgATGGCGGACCTGGACGTTTTTCATGGTTTGCTGCAGCTGTCGACCAAGGCGATCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCAttt                                     <  4:105500/149‑1 (MQ=255)
                                                                                                           cgATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGTCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCAGGTGCAGCCGATGGCAATGGTTACTTAGGCGCGGTTGCTGGCAGCGAAGCGGGGGAACCAttt                                     <  8:266265/149‑1 (MQ=255)
                                                                                                           cgATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCAttt                                     <  5:41183/149‑1 (MQ=255)
                                                                                                            gATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTg                                    <  5:153258/149‑1 (MQ=255)
                                                                                                                   gACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGt                             <  6:83451/149‑1 (MQ=255)
                                                                                                                      cTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCgggg                                              <  6:30894/129‑1 (MQ=255)
                                                                                                                      cTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCgggg                                              >  5:30894/1‑129 (MQ=255)
                                                                                                                      cTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGc                          >  7:203017/1‑149 (MQ=255)
                                                                                                                        ggACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTg                        >  5:240321/1‑149 (MQ=255)
                                                                                                                            gTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGAGTGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCg                                                 >  1:74354/1‑121 (MQ=255)
                                                                                                                            gTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCg                                                 <  2:74354/120‑1 (MQ=255)
                                                                                                                               tttCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTgg                       <  2:320762/143‑1 (MQ=255)
                                                                                                                               tttCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTgg                       >  1:320762/1‑143 (MQ=255)
                                                                                                                                  cAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCg               <  6:240321/148‑1 (MQ=255)
                                                                                                                                   aGGGTTTGGTGCCGCTGTCTACCACGGCGCTGGGTTA‑TTTACACCTAAGGGTGATGGCCGCGGTTGATGCCTATGCAATTGTTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGtt             <  1:288291/149‑1 (MQ=255)
                                                                                                                                    gggTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGc                                                              <  2:60556/99‑1 (MQ=255)
                                                                                                                                    gggTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGc                                                              >  1:60556/1‑99 (MQ=255)
                                                                                                                                    gggTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTg            >  6:358425/1‑149 (MQ=255)
                                                                                                                                     ggTTTGCTGCAGCTGTCGACCAAGGCGCTAGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTg            >  6:245765/1‑148 (MQ=255)
                                                                                                                                       tttGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTAc         <  2:303398/149‑1 (MQ=255)
                                                                                                                                          gctgcCGCTGTCGACCTAGGCGATCGGTGA‑TGTACACCGAAGGGTTAGGTCCGCCGGTGAAGTCGATGTCAAGTGTTAGTTAGGCGCGGTTGCTTGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTACat       <  7:135870/148‑1 (MQ=255)
                                                                                                                                          gctgcAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTACat       <  8:203017/148‑1 (MQ=255)
                                                                                                                                             gcAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGgag                     <  2:353207/131‑1 (MQ=255)
                                                                                                                                             gcAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGgag                     >  1:353207/1‑131 (MQ=255)
                                                                                                                                              cAGCTGTCGACCAAGGGGCGCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTACATATCtt  <  6:35542/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGGCCACACCGACGAACTTGGCTGCCGCCCGGGCATGCAGACCCAGCTTGTTGATGATGGTGATTTCGCGGGCGGGCATCTGGGGCGGATCCTTGGGCTAGAGGTCGCGATGGCGGACCTGGACGTTTTTCAGGGTTTGCTGCAGCTGTCGACCAAGGCGCTCGGTGA‑TGTACACCGAACGGTGATGTCCGCCGGTGCAGCCGATGGCAATGGTTACGTAGGCGCGGTTGCTGGCAGCGAAGCGGGGCAACCATTTGAGCAGGTAGCTGGAGATGTCGTTGTACATATCTT  >  NC_002947/1092748‑1093038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑