Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,245,697 A→G 9.3% S18G (AGC→GGC)  PP_1087 → OmpA family outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,245,6970AG 9.3% 142.8 / 5.0 55S18G (AGC→GGC) PP_1087OmpA family outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base G (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TCAAGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGC  >  NC_002947/1245551‑1245841
                                                                                                                                                  |                                                                                                                                                
tCAAGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGc                                                                                                                                                >  2:94929/1‑149 (MQ=255)
  aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt                                                                                                                                              >  7:110641/1‑149 (MQ=255)
  aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt                                                                                                                                              >  5:90965/1‑149 (MQ=255)
  aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt                                                                                                                                              >  3:97378/1‑149 (MQ=255)
                ccGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCAc                                                                                                                                <  2:178696/149‑1 (MQ=255)
                 cGGAAAACCCCGGGTCGAGCACGGCTATCAAGTTTTCCGGAGAGCGTCCGATTGCCTGCTGGTAAGCCTTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGGCGGCCGGCAGGCTGGCGAAca                                                                                                                               <  4:336890/149‑2 (MQ=255)
                   gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggccg                                                                                                                                      <  6:102578/140‑1 (MQ=255)
                   gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggccg                                                                                                                                      >  5:102578/1‑140 (MQ=255)
                   gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGa                                                                                                                             <  7:198886/149‑1 (MQ=255)
                       aCCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACttt                                                                                                                         <  6:90965/149‑1 (MQ=255)
                                   gCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCCGACGCGCAt                                                                                                             <  6:70884/149‑1 (MQ=255)
                                       ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTGCGCCGGCCGGCAGGCTGGCGAAcag                                                                                                                              >  8:92028/1‑126 (MQ=255)
                                       ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTGCGCCGGCCGGCAGGCTGGCGAAcag                                                                                                                              <  7:92028/128‑3 (MQ=255)
                                          tataAAGTTTTCCGTACAGCCTCCCATAGCCTGCTGGTAACCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCACCGATACCTAGCCCTGTTGACCCTGTTCGCCAGCATGCCGGCCGGCGCACTGACTTTCCAGACGCGAATGGAGaa                                                                                                       >  2:130993/1‑148 (MQ=255)
                                          tATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGaa                                                                                                       >  5:367380/1‑148 (MQ=255)
                                                      cGGACAGCGTCCTTTTGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCAGTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGaa                                                                                           <  1:301526/148‑1 (MQ=255)
                                                       ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAgg                                                                                         >  2:332400/1‑149 (MQ=255)
                                                           aGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGa                                                                                     <  4:97378/149‑1 (MQ=255)
                                                             cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAgg                                                                                   >  3:103976/1‑149 (MQ=255)
                                                                ccGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGt                                                                                 >  8:50023/1‑148 (MQ=255)
                                                                       ccTGCTGGTAAGCCGTCAGAATTTGTGGAGACGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATAGCCTGGAAGGTCGAGGGTGACCAGtt                                                                         >  3:307112/1‑149 (MQ=255)
                                                                        atgctgGTTAGGCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCACTGTTGACCCTGTTCGCCAGCCTGCCGGCCGTCGCACTGACTTTCCAGACGCGAATGGAGAACATTGCCTGGAAGGTCGTGTGTGACCAGTTc                                                                        <  4:307112/148‑1 (MQ=255)
                                                                              gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt                                                                    <  3:333610/147‑1 (MQ=255)
                                                                              gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt                                                                    <  8:110641/147‑1 (MQ=255)
                                                                               tAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTgtc                                                                 >  4:245986/1‑149 (MQ=255)
                                                                                   ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCTTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGcc                                                                                                <  2:303955/114‑1 (MQ=255)
                                                                                      tCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGag                                                          <  3:245986/149‑1 (MQ=255)
                                                                                           aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc                                                     <  6:367380/149‑1 (MQ=255)
                                                                                           aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc                                                     >  6:270397/1‑149 (MQ=255)
                                                                                           aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc                                                     <  4:103976/149‑1 (MQ=255)
                                                                                                  gAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCg                                               >  4:187851/1‑148 (MQ=255)
                                                                                                    gccgccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagcc                                                        >  7:328788/1‑137 (MQ=255)
                                                                                                    gccgccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagcc                                                        <  8:328788/137‑1 (MQ=255)
                                                                                                          cttGCGCCAGCTTTACCTAGCCCTGTTGACCCTGTTCGCCTGCCTGCCGGGCTGCGCACTGACTTTCCAGTCGCGCATGGAGAACATTTCCTGGAATGTCGAGGGTGACCAGTTCGAGTGTAGTCTGAGCCAGCCGATCGAAGGTTTCg                                      <  1:130993/147‑1 (MQ=255)
                                                                                                               gcCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCTAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGc                                 <  1:94929/149‑1 (MQ=255)
                                                                                                                ccAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                <  1:332400/149‑1 (MQ=255)
                                                                                                                         ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg                       >  4:172276/1‑149 (MQ=255)
                                                                                                                         ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg                       <  3:187851/149‑1 (MQ=255)
                                                                                                                          cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAg                                                                                    <  8:157310/87‑1 (MQ=255)
                                                                                                                          cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                      >  3:225722/1‑149 (MQ=255)
                                                                                                                          cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                      <  4:225722/149‑1 (MQ=255)
                                                                                                                              cccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAg                                  >  6:89207/1‑133 (MQ=255)
                                                                                                                              cccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAg                                  <  5:89207/133‑1 (MQ=255)
                                                                                                                                cTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg                <  5:270397/149‑1 (MQ=255)
                                                                                                                                     gACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAg           >  2:62034/1‑149 (MQ=255)
                                                                                                                                        ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc         >  8:121946/1‑148 (MQ=255)
                                                                                                                                           gtgcGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc     >  4:155142/4‑149 (MQ=255)
                                                                                                                                           gTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg                >  4:216570/1‑138 (MQ=255)
                                                                                                                                           gTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg                <  3:216570/138‑1 (MQ=255)
                                                                                                                                              cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagcagc  >  8:176989/1‑149 (MQ=255)
                                                                                                                                              cGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTg                     <  6:105813/130‑1 (MQ=255)
                                                                                                                                              cGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTg                     >  5:105813/1‑130 (MQ=255)
                                                                                                                                               gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGt                              >  1:244959/1‑120 (MQ=255)
                                                                                                                                               gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc     <  6:294495/145‑1 (MQ=255)
                                                                                                                                               gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc     >  5:294495/1‑145 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAAGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGC  >  NC_002947/1245551‑1245841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑