| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,245,701 | T→G | 9.4% | L19R (CTG→CGG) | PP_1087 → | OmpA family outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,245,701 | 0 | T | G | 9.4% | 155.5 / 8.2 | 53 | L19R (CTG→CGG) | PP_1087 | OmpA family outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (22/26); new base G (3/2); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
AAGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTT > NC_002947/1245553‑1245849 | aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt > 3:97378/1‑149 (MQ=255)aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt > 5:90965/1‑149 (MQ=255)aaGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCt > 7:110641/1‑149 (MQ=255) ccGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCAc < 2:178696/149‑1 (MQ=255) cGGAAAACCCCGGGTCGAGCACGGCTATCAAGTTTTCCGGAGAGCGTCCGATTGCCTGCTGGTAAGCCTTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGGCGGCCGGCAGGCTGGCGAAca < 4:336890/149‑2 (MQ=255) gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggccg > 5:102578/1‑140 (MQ=255) gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggccg < 6:102578/140‑1 (MQ=255) gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGa < 7:198886/149‑1 (MQ=255) aCCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACttt < 6:90965/149‑1 (MQ=255) gCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCCGACGCGCAt < 6:70884/149‑1 (MQ=255) ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTGCGCCGGCCGGCAGGCTGGCGAAcag < 7:92028/128‑3 (MQ=255) ggCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTGCGCCGGCCGGCAGGCTGGCGAAcag > 8:92028/1‑126 (MQ=255) tataAAGTTTTCCGTACAGCCTCCCATAGCCTGCTGGTAACCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCACCGATACCTAGCCCTGTTGACCCTGTTCGCCAGCATGCCGGCCGGCGCACTGACTTTCCAGACGCGAATGGAGaa > 2:130993/1‑148 (MQ=255) tATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGaa > 5:367380/1‑148 (MQ=255) cGGACAGCGTCCTTTTGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCAGTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGaa < 1:301526/148‑1 (MQ=255) ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAgg > 2:332400/1‑149 (MQ=255) aGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGa < 4:97378/149‑1 (MQ=255) cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAgg > 3:103976/1‑149 (MQ=255) ccGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGt > 8:50023/1‑148 (MQ=255) ccTGCTGGTAAGCCGTCAGAATTTGTGGAGACGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATAGCCTGGAAGGTCGAGGGTGACCAGtt > 3:307112/1‑149 (MQ=255) atgctgGTTAGGCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCACTGTTGACCCTGTTCGCCAGCCTGCCGGCCGTCGCACTGACTTTCCAGACGCGAATGGAGAACATTGCCTGGAAGGTCGTGTGTGACCAGTTc < 4:307112/148‑1 (MQ=255) gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt < 3:333610/147‑1 (MQ=255) gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt < 8:110641/147‑1 (MQ=255) tAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTgtc > 4:245986/1‑149 (MQ=255) ccGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCTTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGcc < 2:303955/114‑1 (MQ=255) tCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGag < 3:245986/149‑1 (MQ=255) aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc < 4:103976/149‑1 (MQ=255) aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc < 6:367380/149‑1 (MQ=255) aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc > 6:270397/1‑149 (MQ=255) gAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCg > 4:187851/1‑148 (MQ=255) gccgccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagcc < 8:328788/137‑1 (MQ=255) gccgccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagcc > 7:328788/1‑137 (MQ=255) cttGCGCCAGCTTTACCTAGCCCTGTTGACCCTGTTCGCCTGCCTGCCGGGCTGCGCACTGACTTTCCAGTCGCGCATGGAGAACATTTCCTGGAATGTCGAGGGTGACCAGTTCGAGTGTAGTCTGAGCCAGCCGATCGAAGGTTTCg < 1:130993/147‑1 (MQ=255) gcCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCTAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGc < 1:94929/149‑1 (MQ=255) ccAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg < 1:332400/149‑1 (MQ=255) ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg > 4:172276/1‑149 (MQ=255) ccTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCg < 3:187851/149‑1 (MQ=255) cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAg < 8:157310/87‑1 (MQ=255) cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt < 4:225722/149‑1 (MQ=255) cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt > 3:225722/1‑149 (MQ=255) cccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAg < 5:89207/133‑1 (MQ=255) cccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAg > 6:89207/1‑133 (MQ=255) cTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg < 5:270397/149‑1 (MQ=255) gACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAg > 2:62034/1‑149 (MQ=255) ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc > 8:121946/1‑148 (MQ=255) gtgcGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc > 4:155142/4‑149 (MQ=255) gTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg < 3:216570/138‑1 (MQ=255) gTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCg > 4:216570/1‑138 (MQ=255) cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagcagc > 8:176989/1‑149 (MQ=255) cGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTg < 6:105813/130‑1 (MQ=255) cGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTg > 5:105813/1‑130 (MQ=255) gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGt > 1:244959/1‑120 (MQ=255) gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc < 6:294495/145‑1 (MQ=255) gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagc > 5:294495/1‑145 (MQ=255) gCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGGAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGgtg > 8:196537/1‑149 (MQ=255) gCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGgtg > 2:254694/1‑149 (MQ=255) tGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt < 2:126710/121‑1 (MQ=255) tGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt > 1:126710/1‑121 (MQ=255) tGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGttt > 2:353884/1‑149 (MQ=255) | AAGCCCCAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTT > NC_002947/1245553‑1245849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |