Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,294,114	C→T	10.9%	intergenic (+15/+25)	pdxH → / ← PP_1130	pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,294,114	0	C	T	10.9%	146.1 / 7.1	55	intergenic (+15/+25)	pdxH/PP_1130	pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base T (4/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCT  >  NC_002947/1293976‑1294262                                                                                                                                             |                                                                                                                                                      ggggggATTCCGTTTGTGGCCGGAGCGCATCGAGTTCTGGCAGGGGCGGGCTAGTCGTCTGCATGATCGTTTGAACTTTCGGCGTGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAAGTG‑GGGCC‑GCCAAGCGGccc                                                                                                                                              <  2:202132/148‑1 (MQ=255)          ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGa                                                                                                                                    <  4:297697/149‑1 (MQ=255)           cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGAt                                                                                                                                   <  2:194573/149‑1 (MQ=255)            gTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGAGGGCAAGTCGTCTGAATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGTCGCCCTGAGCAACTG‑GGGCC‑GCCAAGAGTCCCCACCCAATGAt                                                                                                                                   >  5:260087/1‑148 (MQ=255)                gttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAg                                                                                                                               <  7:265670/148‑1 (MQ=255)                 ttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAGCTATCGGCTGGTGGACGGGCAGTGGCTGCAAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGc                                                                                                                              >  3:103869/1‑148 (MQ=255)                 ttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCt                                                                                                                             <  6:256274/149‑1 (MQ=255)                     ccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTAccc                                                                                                                         >  1:159435/1‑149 (MQ=255)                           gcATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑a                                                                                                                    <  8:129019/147‑1 (MQ=255)                            cATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATaa                                                                                                                 >  4:155076/1‑149 (MQ=255)                              tCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAAcc                                                                                                               >  1:319888/1‑149 (MQ=255)                                   ttCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCCGGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTgcc                                                                                                           <  1:255591/149‑1 (MQ=255)                                   ttCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACAG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTgccg                                                                                                          >  2:69344/1‑149 (MQ=255)                                        gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGcccc                                                                                                                                             <  2:72465/110‑1 (MQ=255)                                        gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGcccc                                                                                                                                             >  1:72465/1‑110 (MQ=255)                                          aGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTcgcg                                                                                                   >  7:256892/1‑149 (MQ=255)                                                       tcgtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCAcccc                                                                                      <  2:111428/149‑1 (MQ=255)                                                         gtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGc                                                                                    <  1:197475/149‑1 (MQ=255)                                                              cATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGtt                                                                               >  7:37764/1‑149 (MQ=255)                                                              cATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGtt                                                                               >  5:112907/1‑149 (MQ=255)                                                                 gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCt                                                                            >  7:338813/1‑149 (MQ=255)                                                                 gATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCt                                                                            >  8:178275/1‑149 (MQ=255)                                                                   tCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTa                                                                          <  2:319888/149‑1 (MQ=255)                                                                     gTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACAG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGCATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTa                                                                          <  1:69344/148‑1 (MQ=255)                                                                        tGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGcc                                                                      <  6:112907/148‑1 (MQ=255)                                                                             tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTCCCCCGG‑AGAACCTGCCGGCTCGCGCTCCCACCACCCCGCCAGTTGCTTGCGCCCCCCCt                                                                >  4:51777/1‑149 (MQ=255)                                                                                 ggCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGc                                                                                    >  2:35991/1‑125 (MQ=255)                                                                                 ggCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGc                                                                                    <  1:35991/125‑1 (MQ=255)                                                                                         gACGGGCAGTGGCTGCAAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCcgc                                                     <  4:103869/148‑1 (MQ=255)                                                                                          aCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCgcgc                                                   <  8:256892/149‑1 (MQ=255)                                                                                               cAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGAGGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTgc                                               >  1:244596/1‑149 (MQ=255)                                                                                                    gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCgcgc                                                   <  4:138063/139‑1 (MQ=255)                                                                                                    gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCgcgc                                                   >  3:138063/1‑139 (MQ=255)                                                                                                    gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCaa                                          <  8:338813/148‑1 (MQ=255)                                                                                                           gagCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                                  <  2:159435/149‑1 (MQ=255)                                                                                                           gagCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                                  <  7:178275/149‑1 (MQ=255)                                                                                                               ggCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAAc                                                                                                                <  8:272637/67‑1 (MQ=255)                                                                                                               ggCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAAc                                                                                                                >  7:272637/1‑67 (MQ=255)                                                                                                               ggCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                              <  8:37764/149‑1 (MQ=255)                                                                                                                    gcgcCCTGAGCAACTG‑GGGCC‑GCCAAGCGGACCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAAGCACCCCTCCAGTTGCTTAAGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATTAAc                          <  6:260087/148‑1 (MQ=255)                                                                                                                      gcCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCa                               <  8:252083/141‑1 (MQ=255)                                                                                                                      gcCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCa                               >  7:252083/1‑141 (MQ=255)                                                                                                                      gcCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                       >  6:324058/1‑149 (MQ=255)                                                                                                                      gcCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcg                       <  2:244596/149‑1 (MQ=255)                                                                                                                       cccTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCACCCACCCCGCCAGTTGCGTACGCCCCACCTGCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCCTGAAcgcgc                      >  3:208247/1‑149 (MQ=255)                                                                                                                        ccTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgcg                     >  4:322890/1‑149 (MQ=255)                                                                                                                            aatggcTG‑GGGCC‑GCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGtttt                 >  4:32072/6‑149 (MQ=255)                                                                                                                              tggcTG‑GGGCC‑GCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTc               <  3:32072/146‑1 (MQ=255)                                                                                                                              cAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCGTCTCCTGCCGCGCCTGCGCCAACCATTCCAGCATTAACGCGCGtttta                >  6:213961/1‑147 (MQ=255)                                                                                                                               aaCTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGttttt                >  6:127366/1‑147 (MQ=255)                                                                                                                               aaCTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCa              <  3:322890/149‑1 (MQ=255)                                                                                                                                gcTG‑GGGCC‑GCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg             >  2:235703/2‑149 (MQ=255)                                                                                                                                     gggCC‑GCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                                  <  2:229931/124‑1 (MQ=255)                                                                                                                                     gggCC‑GCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                                  >  1:229931/1‑124 (MQ=255)                                                                                                                                     gggCC‑GCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATc         >  2:45857/1‑149 (MQ=255)                                                                                                                                             cAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCt  <  3:155076/149‑1 (MQ=255)                                                                                                                                             cAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCt  >  8:92050/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                      GGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTG‑GGGCC‑GCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGG‑ATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCT  >  NC_002947/1293976‑1294262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑