Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,312,287 C→T 9.3% A790A (GCG→GCA) ‡ rapA ← RNA polymerase‑associated protein RapA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,312,2870CT 9.3% 117.2 / 2.8 43A790A (GCG→GCA) ‡rapARNA polymerase‑associated protein RapA
Reads supporting (aligned to +/- strand):  ref base C (21/18);  new base T (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

CGCGTGACGCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCACGGTCGTAGGTGA  >  NC_002947/1312145‑1312429
                                                                                                                                              |                                                                                                                                               
cgcgTGACGCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTg                                                                                                                                           >  2:210140/1‑149 (MQ=255)
   gTGACGCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTc                                                                                                                                        >  7:291589/1‑149 (MQ=255)
      aCGCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCtt                                                                                                                                      <  1:147092/148‑1 (MQ=255)
        gCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGAt                                                                                                                                   >  3:188941/1‑149 (MQ=255)
         ccAGGTCGTTGCCATTTGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGAt                                                                                                                                   >  7:335356/1‑148 (MQ=255)
         ccAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGAt                                                                                                                                   >  7:330607/1‑148 (MQ=255)
                       ttGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCTgt                                                                                                                    <  4:233428/149‑1 (MQ=255)
                         ggCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGgtg                                                                                                                   <  3:241549/148‑1 (MQ=255)
                          gCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGtt                                                                                                                 <  5:280439/149‑1 (MQ=255)
                            gTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGc                                                                                                               <  4:188941/149‑1 (MQ=255)
                                 gcaggcagCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATc                                                                                                          >  4:43687/1‑149 (MQ=255)
                                      cagCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCAcggcg                                                                                                                      >  2:182978/1‑132 (MQ=255)
                                      cagCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCAcggcg                                                                                                                      <  1:182978/132‑1 (MQ=255)
                                             gTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGc                                                                                              <  7:64830/149‑1 (MQ=255)
                                                 cGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAg                                                                                                       <  2:138677/136‑1 (MQ=255)
                                                 cGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAg                                                                                                       >  1:138677/1‑136 (MQ=255)
                                                        ggcAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCAt                                                                                   <  3:172259/149‑1 (MQ=255)
                                                        ggcAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCAt                                                                                   >  4:172259/1‑149 (MQ=255)
                                                                     ccAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCGTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTAcc                                                                      <  8:330607/149‑1 (MQ=255)
                                                                     ccAGTTGCAGGCTACGGGGTGACACCACCTCGCTGACTAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTAcc                                                                      <  8:335356/149‑1 (MQ=255)
                                                                                        tGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGa                                                  <  3:43687/149‑1 (MQ=255)
                                                                                          ccaccaCCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAg                                                 >  2:59363/1‑148 (MQ=255)
                                                                                          ccaccaCCTCGCTGACGAACAGCAGTTCAAACAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAg                                                 >  8:84274/1‑148 (MQ=255)
                                                                                           caccacCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGTACTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGa                                                  <  4:16542/146‑1 (MQ=255)
                                                                                           caccacCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGTACTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGa                                                  <  4:293382/146‑1 (MQ=255)
                                                                                           caccacCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGTACTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGa                                                  >  3:16542/1‑146 (MQ=255)
                                                                                           caccacCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGTACTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGa                                                  >  3:293382/1‑146 (MQ=255)
                                                                                            accaccTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGaa                                              >  3:6577/1‑149 (MQ=255)
                                                                                                    gCTGACGAACAGCAGTTCAAGCAGGACAGTGGCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATcc                                                                                     >  6:317675/1‑103 (MQ=255)
                                                                                                    gCTGACGAACAGCAGTTCAAGCAGGACAGTGGCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATcc                                                                                     <  5:317675/103‑1 (MQ=255)
                                                                                                    gCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCAt                                        <  4:6577/147‑1 (MQ=255)
                                                                                                          gAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGGTTGCTCCCAGGTGAGGAATTGCATGTCCTcg                                 <  1:210140/149‑1 (MQ=255)
                                                                                                          gAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTcgc                                >  3:230923/1‑149 (MQ=255)
                                                                                                               gcagTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGa                            >  7:204178/1‑148 (MQ=255)
                                                                                                                  gTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAgc                        >  4:326862/1‑149 (MQ=255)
                                                                                                                          aGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAg                                                 >  7:173064/1‑116 (MQ=255)
                                                                                                                          aGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAg                                                 <  8:173064/116‑1 (MQ=255)
                                                                                                                          aGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGc                 >  3:319438/1‑148 (MQ=255)
                                                                                                                           ggACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCa               >  5:335564/1‑149 (MQ=255)
                                                                                                                                gTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCACGGTc          <  4:230923/149‑1 (MQ=255)
                                                                                                                                     tGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGCCAGCGCCTGGCCACGGTCGTAgg     >  6:354641/1‑149 (MQ=255)
                                                                                                                                           gAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCACGGTCGTAGGTGa  <  3:304877/146‑1 (MQ=255)
                                                                                                                                           gAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCACGGTCGTAGGTGa  >  4:304877/1‑146 (MQ=255)
                                                                                                                                              cgcTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCAt                                        <  4:87799/105‑1 (MQ=255)
                                                                                                                                              cgcTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCAt                                        >  3:92532/1‑105 (MQ=255)
                                                                                                                                              cgcTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCAt                                        >  3:87799/1‑105 (MQ=255)
                                                                                                                                              cgcTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCAt                                        <  4:92532/105‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CGCGTGACGCCAGGTCGTTGCCATTGGCGTCGAGCAGGCAGCGCAGTGCCGCTGGCGGCAGGTAGCGGCCCAGTTGCAGGCTACGGGGTGCCACCACCTCGCTGACGAACAGCAGTTCAAGCAGGACAGTGCCTGGTTTGAGCGCTTTGTTCTTGATCAACGCCACGGCGGTGTTGCCCATCGAGCCTGACAGCACCAGATCCATGCCGCCCTGTACCATCGGG‑TGCTCCCAGGTGAGGAATTGCATGTCCTCGCGTGACAGCGCCTGGCCACGGTCGTAGGTGA  >  NC_002947/1312145‑1312429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑