| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,392,938 | C→T | 12.5% | intergenic (+108/‑78) | PP_1214 → / → ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,392,938 | 0 | C | T | 12.5% | 81.3 / 3.0 | 32 | intergenic (+108/‑78) | PP_1214/ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Reads supporting (aligned to +/- strand): ref base C (17/11); new base T (2/2); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
CCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGC > NC_002947/1392794‑1393083 | ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTa > 2:272991/1‑149 (MQ=255) cAGATTTCCGACGAGATCATGGAATACCTCGGCTAAGGTTATTTCGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTAc < 3:10572/149‑1 (MQ=255) ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAg < 2:250068/148‑1 (MQ=255) tGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc < 1:272991/149‑1 (MQ=255) tGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc < 3:318286/149‑1 (MQ=255) ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGc > 6:106360/1‑139 (MQ=17) ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGc < 5:106360/139‑1 (MQ=17) cTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc > 1:62796/1‑132 (MQ=255) cTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc < 2:62796/132‑1 (MQ=255) tttGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg > 1:23463/1‑120 (MQ=255) tttGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg < 2:23463/120‑1 (MQ=255) gggTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAcc > 6:79038/1‑149 (MQ=255) ggTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc > 3:36528/1‑149 (MQ=255) cccGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc > 2:230271/1‑149 (MQ=255) ccGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg > 1:58206/1‑149 (MQ=255) tCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc < 2:203103/146‑1 (MQ=18) tCCTACAGGTACTGCACCACATTTGAAAAG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc > 1:203103/1‑146 (MQ=18) aGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCg > 2:307351/1‑149 (MQ=255) aGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCg > 5:160711/1‑149 (MQ=255) aGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCg < 5:79038/149‑1 (MQ=255) aTTACGCC‑CATTTCAATTGTGGGTCTGTACGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt < 4:36528/148‑1 (MQ=39) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTc > 5:328329/1‑110 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTc < 6:328329/110‑1 (MQ=255) gCC‑CATATCAAATCTGCTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGATGTTTGAACAGATGCATCATTTTTTCGACCGCCTTAAACACACTGATTCTAGGAATCGACCCCGTATCGCGAAACACCGGCTATGGCg > 2:136813/1‑149 (MQ=25) cc‑cATTTAAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGAGCCGGCGCAGTTTGTAGAGATGCATAATTTTTTCGAAGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTATCTCATCACCGGCTAtttcgt > 8:225626/1‑144 (MQ=38) c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg > 6:48147/1‑149 (MQ=255) ttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc > 8:52289/1‑149 (MQ=255) aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGTGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGc < 7:225626/148‑1 (MQ=255) aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACGCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGc > 6:169290/1‑148 (MQ=255) aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGc < 6:160711/148‑1 (MQ=255) aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGc > 6:96503/1‑148 (MQ=255) gtgCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGc > 7:205363/1‑149 (MQ=255) | CCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGC > NC_002947/1392794‑1393083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |