Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,411,292	G→A	6.5%	P215S (CCG→TCG)	PP_1232 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,411,292	0	G	A	6.5%	177.7 / 2.1	61	P215S (CCG→TCG)	PP_1232	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/26);  new base A (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

TGCTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGCCCGCATCGCCGGCA  >  NC_002947/1411145‑1411440                                                                                                                                                     |                                                                                                                                                     tGCTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCACTCGAACATGGTCGGc                                                                                                                                                     <  5:315520/149‑1 (MQ=255)  gCTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCgg                                                                                                                                                      <  8:39913/147‑1 (MQ=255)  gCTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCgg                                                                                                                                                      >  7:39913/1‑147 (MQ=255)   cTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATTGTCGGCAt                                                                                                                                                   <  5:16682/149‑1 (MQ=255)        ttCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGc                                                                                                                                              >  2:183431/1‑149 (MQ=255)           aaTGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGt                                                                                                                                            <  8:210502/148‑1 (MQ=255)                cACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCg                                                                                                                                      >  6:341736/1‑149 (MQ=255)                cACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCg                                                                                                                                      >  1:104839/1‑149 (MQ=255)                   ccTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTCGAGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCg                                                                                                                                      >  1:287776/1‑147 (MQ=255)                   ccTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAg                                                                                                                                   >  1:24894/1‑149 (MQ=255)                       gggCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGcc                                                                                                                                 <  5:341736/147‑1 (MQ=255)                              tGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTc                                                                                                                        >  2:78541/1‑149 (MQ=255)                                gTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTcc                                                                                                                       <  2:24894/148‑1 (MQ=255)                                      gcgGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCt                                                                                                                <  1:78541/149‑1 (MQ=255)                                        ggTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATc                                                                                                                                       <  2:353004/124‑1 (MQ=255)                                        ggTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATc                                                                                                                                       >  1:353004/1‑124 (MQ=255)                                              tggTGTCGGCAATACGTGATTCGGGCACCGGGTGGGTC‑AGCATGAATTCCGGAGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATAc                                                                                                          <  4:14811/146‑1 (MQ=255)                                              gggTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCCGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATAcc                                                                                                         >  6:141318/1‑148 (MQ=255)                                                gtctCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGCCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATAc                                                                                                          >  3:14811/4‑145 (MQ=255)                                                 tgtCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATg                                                                                                     >  1:190516/1‑149 (MQ=255)                                                  gtCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGc                                                                                                    >  7:4788/1‑149 (MQ=255)                                                     ggCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGAt                                                                                                            >  2:299269/1‑138 (MQ=255)                                                     ggCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGAt                                                                                                            <  1:299269/138‑1 (MQ=255)                                                      gCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCg                                                                                                   <  5:283928/146‑1 (MQ=255)                                                      gCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCg                                                                                                   >  6:283928/1‑146 (MQ=255)                                                       cAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGGGGGGCGACATAGCGAGACTGGCGTTCCAGGCGCACTACCATGGTAGGGAGGTTGCGTGGATCTTCGGCGGGTTGTTCAAGGTGCTGGAGACCGATGccggcg                                                                                               >  8:139960/1‑144 (MQ=255)                                                          tACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCt                                                                                            >  3:142240/1‑149 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGc                                                                                                                                              <  7:205562/96‑1 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGc                                                                                                                                              >  8:205562/1‑96 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTgg                                                                                                              >  6:114906/1‑128 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTgg                                                                                                              <  5:114906/128‑1 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTcc                                                                                         >  6:153934/1‑149 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTcc                                                                                         <  2:138283/149‑1 (MQ=255)                                                             gTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTcc                                                                                         <  4:31611/149‑1 (MQ=255)                                                                aTTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCTGTCGGCTTCCTGt                                                                                      <  2:93654/149‑1 (MQ=255)                                                                  tCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGGCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGtt                                                                                     <  2:190516/148‑1 (MQ=255)                                                                       cACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCt                                                                               >  6:69958/1‑149 (MQ=255)                                                                              tgggtC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCga                                                                        <  2:104839/149‑1 (MQ=255)                                                                                      gcagGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAgcgc                                                                 <  5:153934/149‑1 (MQ=255)                                                                                               ccGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTc                                                         <  2:322276/148‑1 (MQ=255)                                                                                                cggcggTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTcc                                                        <  7:492/148‑1 (MQ=255)                                                                                                     gTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATg                                                  <  5:170752/149‑1 (MQ=255)                                                                                                         ggCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGcc                                               <  1:183431/148‑1 (MQ=255)                                                                                                                 aGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGGATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGTCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTgg                                      >  1:217691/1‑149 (MQ=255)                                                                                                                 aGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTgg                                      >  2:49357/1‑149 (MQ=255)                                                                                                                 aGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGACATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTgg                                      >  2:336229/1‑149 (MQ=255)                                                                                                                 aGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGACATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTgg                                      >  2:341566/1‑149 (MQ=255)                                                                                                                  gCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGAATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTggg                                     >  1:240834/1‑149 (MQ=255)                                                                                                                   cGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGt                                    <  5:69958/149‑1 (MQ=255)                                                                                                                      tACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGcc                                 >  7:165073/1‑149 (MQ=255)                                                                                                                        cTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCgg                               <  4:142240/149‑1 (MQ=255)                                                                                                                            cGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCt                                            >  1:139599/1‑132 (MQ=255)                                                                                                                                ccAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGAAGCGGTCGGCTTCCTGTTCATTCTGGCGAGAGAAGCGCCGTTGATCCTGAATGGCCGCTGACTGGGTGCCGGCAATCATg                       >  5:72267/1‑149 (MQ=255)                                                                                                                                 cAGGCGCTCGAACATGGTCGACATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGc                      <  1:341566/149‑1 (MQ=255)                                                                                                                                 cAGGCGCTCGAACATGGTCGACATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGc                      <  1:336229/149‑1 (MQ=255)                                                                                                                                  aGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGcc                     >  4:149340/1‑149 (MQ=255)                                                                                                                                   ggCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGcc                     >  4:146925/1‑148 (MQ=255)                                                                                                                                   ggCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGcc                     >  3:353011/1‑148 (MQ=255)                                                                                                                                        tCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGcc               >  5:103767/1‑149 (MQ=255)                                                                                                                                        tCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGcc               >  8:174835/1‑149 (MQ=255)                                                                                                                                               tGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGCCCGCATCg        <  1:49357/149‑1 (MQ=255)                                                                                                                                                   cGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCaa                            >  4:39970/1‑125 (MQ=255)                                                                                                                                                   cGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCaa                            <  3:39970/125‑1 (MQ=255)                                                                                                                                                     gCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGCCCGCATCGCCGGca  <  3:149340/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGCTGTCTTCAATGCCACCCTTGGGCGCCTGGTCGGCGCGGTTACGGGTGTCGGCAATACGTGATTCGGTCACCGGGTGGGTC‑AGCAGGAATTCCGGCGGTTTGGCGTCATAGCGATACTGGCGTGCCAGGCGCTCGAACATGGTCGGCATGTTGCGTGGATCGTAGCCGGCTTTTTCCAGGTTCTGGATACCGATGCGGTCGGCTTCCTGTTCATTCTGGCGCGAGAAGCGCCGTTGCTCCTGAATGGCCGCTGCCTGGGTGCCGGCAATCATGCCGATGCCCGCATCGCCGGCA  >  NC_002947/1411145‑1411440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑