| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 156,386 | T→C | 10.1% | intergenic (‑11/+32) | ygdQ ← / ← citN | transport protein/citrate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 156,386 | 0 | T | C | 10.1% | 154.9 / 6.5 | 59 | intergenic (‑11/+32) | ygdQ/citN | transport protein/citrate transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/25); new base C (3/3); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CAGAATCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGCCGAACT > NC_002947/156247‑156527 | cAGAATCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCTGACCGGGgcgc < 6:148546/148‑1 (MQ=255)cAGAATCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCTGACCGGGgcgc > 5:148546/1‑148 (MQ=255) gAATCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGcgcgtc < 5:358190/149‑1 (MQ=255) aaTCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCTGACCGGGGcgcgttg < 4:178678/149‑3 (MQ=255) aTCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCATTTTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGcgcgtcgc < 3:240660/149‑1 (MQ=255) gagaTCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTa > 3:7164/1‑149 (MQ=255) agaTCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTAc > 8:162404/1‑149 (MQ=255) gatgatgTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATAc > 5:198087/1‑149 (MQ=255) gATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTaaa > 7:151266/1‑149 (MQ=255) cGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGacta < 3:94859/149‑1 (MQ=255) ggACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTg < 2:248856/149‑1 (MQ=255) gATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCTGACCGGGGcgcgttgcc > 8:255361/1‑114 (MQ=255) gATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCTGACCGGGGcgcgttgcc < 7:255361/115‑2 (MQ=255) aaGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCggg < 8:151266/148‑1 (MQ=255) gTAATGGTTAAAAAGGCACCCCAGATTTCCGGGCTGGTCCGCCAGTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATccc < 8:59887/149‑1 (MQ=255) gTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTa > 8:216290/1‑104 (MQ=255) gTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTa < 7:216290/104‑1 (MQ=255) ggTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATca < 5:60593/149‑1 (MQ=255) gTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATcag < 6:198087/149‑1 (MQ=255) gTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATcag > 1:220161/1‑149 (MQ=255) tGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCggg > 2:308351/1‑134 (MQ=255) tGAAAAAGGCAACCCAGATTTCCGGGCGGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATcagc > 8:323647/1‑149 (MQ=255) aaaGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAACTcccc < 1:100058/139‑1 (MQ=255) aaaGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATcccc > 2:100058/1‑139 (MQ=255) aaCCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGa < 4:7164/149‑1 (MQ=255) tcccAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAg < 3:198288/148‑1 (MQ=255) ccAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCa > 5:264349/1‑149 (MQ=255) ccAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATAAGCAGTGCGCCGAg > 4:198288/1‑147 (MQ=255) aGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCAt > 4:60499/1‑148 (MQ=255) gATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTa < 6:264349/149‑1 (MQ=255) cGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGca > 6:103607/1‑147 (MQ=255) tCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATAc > 3:316614/1‑148 (MQ=255) tCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATAc < 7:162404/148‑1 (MQ=255) aGCCATTCCATGTATTTTCCTTCGAGCGGTAATGACAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAg > 3:227077/1‑149 (MQ=255) ccATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCa < 2:220161/149‑1 (MQ=255) cATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGcc < 5:103607/148‑1 (MQ=255) aTGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCa > 3:335699/1‑149 (MQ=255) tGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCg > 5:320793/1‑90 (MQ=255) tGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCg < 6:320793/90‑1 (MQ=255) aTTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACtt > 7:218760/1‑148 (MQ=255) ttttCCTTCGAGCGGTAATGGCAACACGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACtt > 8:256749/1‑147 (MQ=255) tttCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATg < 2:284061/120‑1 (MQ=255) tttCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATg > 1:284061/1‑120 (MQ=255) ttCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGcac > 2:232171/1‑122 (MQ=255) ttCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGcac < 1:232171/122‑1 (MQ=255) ttCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTg > 5:175946/1‑148 (MQ=255) gTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCtga < 3:60499/149‑1 (MQ=255) gTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCtga < 4:316614/149‑1 (MQ=255) tAATGGCAACGCGCCCCTGTCAGGGCGCTTCGCGTACGTGATACTGTTGTATTAAATACTACTGAACAGCGGGAAAATCCCCATCATCAGTGCGCCGAGCATTATGCACAGGCa < 8:319135/114‑1 (MQ=255) tAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCa > 7:319135/1‑114 (MQ=255) tAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCtgat < 7:323647/149‑1 (MQ=255) tGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTgatgat > 1:193161/1‑149 (MQ=255) gCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCt < 5:289732/141‑1 (MQ=255) gCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCt > 6:289732/1‑141 (MQ=255) gaCGCGCCCTGACCGGGGCGCGTTGCGTACGTGATACGGATGTATAAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGcc > 8:197927/2‑149 (MQ=255) aaCGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGcc > 5:189616/1‑149 (MQ=255) aaCGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGcc > 2:251274/1‑149 (MQ=255) cgcCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGCCGAAc < 6:175946/149‑1 (MQ=255) gcCCCGGTCAGGGCGCGTCGCGTACGTGAGACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCAGACCAGCACTGCCCACTTGAGCGGGAAGCGCTGATGAGCGCCGAACt > 6:357387/1‑149 (MQ=255) | CAGAATCGAGATCATGATGATGTTGTCGATACCGAGGACGATCTCAAGCGCGGTAAGGGTGAAAAAGGCAACCCAGATTTCCGGGCTGGTCAGCCATTCCATGTATTTTCCTTCGAGCGGTAATGGCAACGCGCCCCGGTCAGGGCGCGTCGCGTACGTGATACGGATGTATTAAAGACTACTGAACAGCGGGAAAATCCCCATCAGCAGTGCGCCGAGCATTATGCACAGGCATACCAGCACTGCCCACTTGAGCGTGAAGCGCTGATGATCGCCGAACT > NC_002947/156247‑156527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |