Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,424,252	G→C	11.1%	E321D (GAG→GAC) ‡	PP_5467 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,424,252	0	G	C	11.1%	172.3 / 11.6	63	E321D (GAG→GAC) ‡	PP_5467	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base C (4/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

CAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGT  >  NC_002947/1424105‑1424398                                                                                                                                                    |                                                                                                                                                   cAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAgg                                                                                                                                                   >  6:62862/1‑149 (MQ=255)    gagTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCtt                                                                                                                                                >  3:216420/1‑149 (MQ=255)     agTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                               <  6:6389/149‑1 (MQ=255)       tGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTc                                                                                                                                              <  5:118203/148‑1 (MQ=255)         cccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGc                                                                                                                                           >  1:306935/1‑149 (MQ=255)         cccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                           <  4:282376/149‑1 (MQ=255)          ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCg                                                                                                                                          <  2:306935/149‑1 (MQ=255)           cTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCg                                                                                                                                          >  2:340344/1‑148 (MQ=255)           cTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCg                                                                                                                                          >  3:67785/1‑148 (MQ=255)            tGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGaa                                                                                                                                        >  5:183361/1‑149 (MQ=255)              aGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGTTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGaa                                                                                                                                        <  4:253958/147‑1 (MQ=255)                 gcgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACca                                                                                                                                   <  3:46491/149‑1 (MQ=255)                   gATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAagag                                                                                                                                                    >  2:146514/1‑130 (MQ=255)                   gATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAagag                                                                                                                                                    <  1:146514/130‑1 (MQ=255)                      gCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcag                                                                                                                                  <  7:8624/145‑1 (MQ=255)                      gCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcag                                                                                                                                  >  8:8624/1‑145 (MQ=255)                              gcgcGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGGGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTcccc                                                                                                                      <  4:216420/149‑1 (MQ=255)                                     cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAg                                                                                                                   <  2:17082/145‑1 (MQ=255)                                     cAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAg                                                                                                                   >  1:17082/1‑145 (MQ=255)                                       gCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAg                                                                                                             >  5:301540/1‑149 (MQ=255)                                              cacCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAg                                                                                                      >  4:335503/1‑149 (MQ=255)                                              cacCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAg                                                                                                      >  4:317503/1‑149 (MQ=255)                                               acCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGc                                                                                                     >  7:221441/1‑149 (MQ=255)                                                ccGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGcc                                                                                                    >  2:289053/1‑149 (MQ=255)                                                        gCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGc                                                                                              >  2:89029/1‑147 (MQ=255)                                                         cGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTGCAGCGAATACCAGGAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCag                                                                                           <  5:62862/149‑1 (MQ=255)                                                          ggTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCag                                                                                           >  1:55684/1‑148 (MQ=255)                                                           gtgtTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagga                                                                                         <  6:301540/149‑1 (MQ=255)                                                           gtgtTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagga                                                                                         <  3:333169/149‑1 (MQ=255)                                                            tgtTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCaggag                                                                                        <  1:89029/149‑1 (MQ=255)                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagca                                                                                                                                <  8:113594/104‑1 (MQ=255)                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcagca                                                                                                                                >  7:113594/1‑104 (MQ=255)                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                   <  1:92749/149‑1 (MQ=255)                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTgg                                                                                   >  3:138968/1‑149 (MQ=255)                                                                 gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTg                                                                                    <  8:67064/148‑1 (MQ=255)                                                                    gAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGATCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTggg                                                                                  <  7:185506/147‑1 (MQ=255)                                                                    gAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCACAAGGAAAAGTTCGCAGCCTTCTGCCAGGAGGATggg                                                                                  >  8:185506/1‑147 (MQ=255)                                                                     aaGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                                <  3:278358/132‑1 (MQ=255)                                                                     aaGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                                >  4:278358/1‑132 (MQ=255)                                                                     aaGCGGGGAGCGGCTTTGCCCGCAAGGCCAAGCTCGACCACTACTCGGTCAGCTACAGCGGCTTCAAATCCCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTg                                                                               <  8:198130/149‑1 (MQ=255)                                                                          gTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCtt                                                                                                  <  2:154726/125‑1 (MQ=255)                                                                          gTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCtt                                                                                                  >  1:154726/1‑125 (MQ=255)                                                                             aGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTg                                                                       <  1:289053/149‑1 (MQ=255)                                                                                ggCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                    <  4:138968/149‑1 (MQ=255)                                                                                 gCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                    >  1:234889/1‑148 (MQ=255)                                                                                  cTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGat                                                                                                                                        >  6:356626/1‑78 (MQ=38)                                                                                  cTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGat                                                                                                                                        <  5:356626/79‑2 (MQ=38)                                                                                  cTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCATCTGCCAGGAGGTTGGGCTGTCGTCGTGGAg                                                                    >  4:34092/1‑147 (MQ=255)                                                                                        ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTAGGGCTGTCGTCGTGGAGCAGTGACt                                                            >  4:74668/1‑149 (MQ=255)                                                                                         cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                           <  2:75246/149‑1 (MQ=255)                                                                                                 cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgcc                                                    >  4:45926/1‑148 (MQ=255)                                                                                                             ctactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAAcc                                       <  2:234889/149‑1 (MQ=255)                                                                                                              tactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACca                                      >  3:291306/1‑149 (MQ=255)                                                                                                                   ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGGAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                   <  3:317503/147‑1 (MQ=255)                                                                                                                   ggTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAg                                   <  3:335503/147‑1 (MQ=255)                                                                                                                              gCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATgc                      <  4:67785/149‑1 (MQ=255)                                                                                                                                 gCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATgcgc                    >  1:95362/1‑148 (MQ=255)                                                                                                                                  cTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCa                  >  2:31397/1‑149 (MQ=255)                                                                                                                                   ttCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCAt                 >  6:335338/1‑149 (MQ=255)                                                                                                                                      aaaTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCgg              >  7:219706/1‑149 (MQ=255)                                                                                                                                      aaaTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCg               <  3:74668/148‑1 (MQ=255)                                                                                                                                          cGCTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCggg             >  8:240148/1‑146 (MQ=255)                                                                                                                                            cTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTgtcgtc                                                                          <  7:75332/83‑1 (MQ=255)                                                                                                                                            cTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTgtcgtc                                                                          >  8:75332/1‑83 (MQ=255)                                                                                                                                             tGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAg       >  3:183167/1‑149 (MQ=255)                                                                                                                                               aaaGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCATCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAg       <  3:34092/147‑1 (MQ=255)                                                                                                                                                  gagGCTTTCAGCGAATACCAGCAGCAAGGCCCCAAGGCACAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGt  <  6:183361/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CAAGAGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGCAGAAGCTGGT  >  NC_002947/1424105‑1424398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑