Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,474,995 T→G 8.1% intergenic (‑775/+26) algD ← / ← PP_1289 GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,474,9950TG 8.1% 166.3 / 5.2 60intergenic (‑775/+26)algD/PP_1289GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base G (2/3);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGA  >  NC_002947/1474849‑1475142
                                                                                                                                                  |                                                                                                                                                   
aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGAGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCtt                                                                                                                                                     >  7:70935/1‑147 (MQ=255)
 aaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGAAGGGCAGAGGAGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                                  >  8:37282/1‑149 (MQ=255)
      ggTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCcgcg                                                                                                                                             <  5:113322/149‑1 (MQ=255)
        tATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGaa                                                                                                                                           <  1:142811/149‑1 (MQ=255)
        tATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGGCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                           <  2:266496/149‑1 (MQ=255)
         aTGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCcgcg                                                                                                                                             >  1:321882/1‑146 (MQ=255)
            aaCGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAagag                                                                                                                                        <  5:362766/148‑1 (MQ=255)
            aaCGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAgg                                                                                                                                       <  7:196194/149‑1 (MQ=255)
            aaCGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                        <  2:95256/148‑1 (MQ=255)
              cGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGc                                                                                                                                     >  3:267412/1‑149 (MQ=255)
               ggTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCg                                                                                                                                    >  1:210612/1‑149 (MQ=255)
               ggTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCg                                                                                                                                    >  2:293966/1‑149 (MQ=255)
                gTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGt                                                                                                                                   <  1:293966/149‑1 (MQ=255)
                 tCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTg                                                                                                                                  >  6:224060/1‑149 (MQ=255)
                     aTGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACa                                                                                                                               <  8:46194/148‑1 (MQ=255)
                                   tAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                                <  6:39222/149‑1 (MQ=255)
                                     aaGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCgggg                                                                                                               <  2:79830/148‑1 (MQ=255)
                                        ggAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACt                                                                                                                       <  2:90100/137‑1 (MQ=255)
                                        ggAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACt                                                                                                                       >  1:90100/1‑137 (MQ=255)
                                               cGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAg                                                                                                    <  4:161047/149‑1 (MQ=255)
                                                gACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAgg                                                                                                   <  3:303462/149‑1 (MQ=255)
                                                 aCGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAgg                                                                                                   >  8:298865/1‑148 (MQ=255)
                                                    gATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACa                                                                                                                               <  1:166910/117‑1 (MQ=255)
                                                    gATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACa                                                                                                                               >  2:166910/1‑117 (MQ=255)
                                                          tGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCaaa                                                                                          <  5:224060/148‑1 (MQ=255)
                                                             cGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGt                                                                                      <  7:263101/149‑1 (MQ=255)
                                                              gTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCgcgc                                                                                                      >  8:159723/1‑132 (MQ=255)
                                                              gTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCgcgc                                                                                                      <  7:159723/132‑1 (MQ=255)
                                                               tCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCt                                                                                    <  7:184943/149‑1 (MQ=255)
                                                                      cGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              <  4:267412/148‑1 (MQ=255)
                                                                                    ggTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTg                                                               <  2:210612/149‑1 (MQ=255)
                                                                                    ggTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTg                                                               <  6:215096/149‑1 (MQ=255)
                                                                                          cAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagta                                                          <  7:298865/148‑1 (MQ=255)
                                                                                           aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTg                                                               >  6:124324/1‑142 (MQ=255)
                                                                                           aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTg                                                               <  5:124324/142‑1 (MQ=255)
                                                                                             tctGCCCTGCAACAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGt                                                                                                              >  6:352100/3‑93 (MQ=255)
                                                                                             tctGCCCTGCAACAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGt                                                                                                              <  5:352100/91‑1 (MQ=255)
                                                                                                  ccTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCt                                                  >  1:7276/1‑148 (MQ=255)
                                                                                                        gCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCAt                                            <  2:151833/148‑1 (MQ=255)
                                                                                                               agagGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGc                                    >  7:234239/1‑149 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              >  4:104688/1‑101 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              <  3:109594/101‑1 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              <  3:104688/101‑1 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              >  4:109594/1‑101 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                >  4:342798/1‑131 (MQ=255)
                                                                                                                     ggATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                <  3:342798/131‑1 (MQ=255)
                                                                                                                        tCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTCTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGt                           >  5:343814/1‑149 (MQ=255)
                                                                                                                              cggcTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg                     <  6:92712/149‑1 (MQ=255)
                                                                                                                                     ccTCTTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                <  3:205409/115‑1 (MQ=255)
                                                                                                                                     ccTCTTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                >  4:205409/1‑115 (MQ=255)
                                                                                                                                      ctctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGAt             >  3:27487/1‑149 (MQ=255)
                                                                                                                                        ctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAc                                                      >  4:177821/1‑106 (MQ=255)
                                                                                                                                        ctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAc                                                      <  3:177821/106‑1 (MQ=255)
                                                                                                                                        ctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgc           >  2:193424/1‑149 (MQ=255)
                                                                                                                                         ttCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCtt                                  <  3:345963/125‑1 (MQ=255)
                                                                                                                                         ttCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCtt                                  >  4:345963/1‑125 (MQ=255)
                                                                                                                                         ttCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgcg          >  3:10735/1‑149 (MQ=255)
                                                                                                                                            gcgGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCgctg       >  4:315439/1‑149 (MQ=255)
                                                                                                                                              ggCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGa                                <  4:136499/122‑1 (MQ=255)
                                                                                                                                              ggCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGa                                >  3:136499/1‑122 (MQ=255)
                                                                                                                                               gCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGAGctgctg    >  7:314857/1‑149 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACCGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGa  >  3:32497/1‑149 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                <  4:147618/103‑1 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                                >  3:147618/1‑103 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg                     <  4:169229/130‑1 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg                     >  3:169229/1‑130 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGA  >  NC_002947/1474849‑1475142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑