| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,521,248 | C→A | 9.0% | Q169K (CAG→AAG) | murD → | UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,521,248 | 0 | C | A | 9.0% | 233.3 / 12.0 | 78 | Q169K (CAG→AAG) | murD | UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase |
| Reads supporting (aligned to +/- strand): ref base C (40/31); new base A (3/4); total (43/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01 | |||||||||||
CAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACCG > NC_002947/1521106‑1521396 | cAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCGAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTg > 8:109561/1‑148 (MQ=255) aaGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTgg > 4:73221/1‑148 (MQ=255) aaGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTgg > 8:10765/1‑148 (MQ=255) agagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAgctggaagctggaa < 5:257628/149‑1 (MQ=255) gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACCATGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAgctggaagctggaa < 1:283805/149‑1 (MQ=255) gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGaaa > 6:165042/1‑149 (MQ=255) gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa < 8:341429/149‑1 (MQ=255) cACCGTCACGACGGTGGTCGGTGAAATGGGAGCCAAGGCCGGCAAACGCGTGGCCGTGTGGTGTAAGCTCTGTACCCCGTCA‑CTGGACCTGCTGTCCGATGATATCGAGCTGTACGTGGTGGAGCTTTCCAGGTTCCAGCTGGAAAc < 8:265729/147‑1 (MQ=255) cACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAAc > 7:265729/1‑147 (MQ=255) agcGTCACGACGCTGCTCGGTGAAATGGCCGACAACGCCGGCAAACTCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGTAAACCAc < 7:315026/147‑1 (MQ=255) aCCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCTGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCAc < 1:35350/149‑1 (MQ=255) aCCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctc < 3:207085/149‑5 (MQ=255) tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc > 1:20224/1‑149 (MQ=255) tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc < 2:136317/149‑1 (MQ=255) tGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAAc > 3:30721/1‑149 (MQ=255) tGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATAGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAAc > 6:22694/1‑149 (MQ=255) gTCGGTGAAATGGCCGCCAATGCCGGCAAACGCGTGGCCGTGGGCGGTAACCACGCTCCCCCCGCA‑CTCGACCTGCTGGCCGCTGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGc < 8:157547/149‑1 (MQ=255) ggTGAAATGGCCTCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCg < 4:105998/148‑1 (MQ=255) ggTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCg > 8:304677/1‑148 (MQ=255) aaTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTgg < 3:73221/149‑1 (MQ=255) cAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTc < 1:118075/149‑1 (MQ=255) aaGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCTGAAGTGGCGACCGTTCt < 5:22694/149‑1 (MQ=255) aaGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCt < 7:35772/149‑1 (MQ=255) ggCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTg > 3:220201/1‑148 (MQ=255) ccGGCAACCGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGaa > 6:52144/1‑148 (MQ=255) cGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTAAACGCCGAAGTGGCGACCGTTCAGACCa > 8:95484/1‑149 (MQ=255) ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGa < 4:30247/136‑1 (MQ=255) ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGa > 3:30247/1‑136 (MQ=255) ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACAt < 4:220201/149‑1 (MQ=255) ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACAt < 7:10765/149‑1 (MQ=255) cAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCa < 7:304677/149‑1 (MQ=255) cAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGAA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACAt > 6:204820/1‑147 (MQ=255) aaaCGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAg < 4:30721/149‑1 (MQ=255) cGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctc < 7:301933/96‑5 (MQ=39) cGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctc > 8:301933/1‑92 (MQ=39) gTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACAt > 6:360323/1‑149 (MQ=255) gggCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATg > 3:162537/1‑148 (MQ=255) gggCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCAAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATg > 8:210885/1‑148 (MQ=255) gctcGGTAACCTCGGTACCCAGGCA‑CTCGACCTGCTGGCCGATTACATCGAGCTGTACGTGCTGGAGCTTTCAAGCTTCCAGCTGGAAACCACTGACCAGCTAAACGCCTAAGTTGCTAAAGTTCTGAACATCAGCGAATACCACATg > 8:216497/4‑148 (MQ=255) ggCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGa < 3:255159/127‑1 (MQ=255) ggCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGa > 4:255159/1‑127 (MQ=255) gCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGAc > 8:134769/1‑149 (MQ=255) cGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGACCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGAcc > 3:305808/1‑149 (MQ=255) aCCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCt > 4:96443/1‑149 (MQ=255) cccGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGAc > 4:141535/1‑102 (MQ=255) cccGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc > 1:291118/1‑149 (MQ=255) gCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGc < 4:162537/149‑1 (MQ=255) a‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGcc > 8:163419/1‑148 (MQ=255) a‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCt < 7:163419/149‑1 (MQ=255) tCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCAc > 8:122236/1‑67 (MQ=255) tCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCAc < 7:122236/67‑1 (MQ=255) tCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTac > 3:162188/1‑149 (MQ=255) gACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTacc < 7:109561/148‑1 (MQ=255) tgctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCg < 7:80815/129‑1 (MQ=255) tgctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCg > 8:80815/1‑129 (MQ=255) ctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTg < 4:162188/147‑1 (MQ=255) ccGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCaaa < 5:360323/149‑1 (MQ=255) aCATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCg < 5:52144/148‑1 (MQ=255) cATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGa > 1:343495/1‑149 (MQ=255) cATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGa < 4:305808/149‑1 (MQ=255) gAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAtttt > 6:91382/1‑149 (MQ=255) cTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcg < 2:343495/149‑1 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcgcg < 7:295375/148‑1 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 8:325897/1‑149 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 8:211534/1‑149 (MQ=255) tACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCgg > 6:75208/1‑149 (MQ=255) cGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTaa > 7:301813/1‑117 (MQ=255) cGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTaa < 8:301813/118‑2 (MQ=255) gTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGc > 3:299126/1‑149 (MQ=255) cTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGccc < 3:96443/148‑1 (MQ=255) tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGa < 8:48805/132‑1 (MQ=255) tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGa > 7:48805/1‑132 (MQ=255) tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc > 3:312850/1‑149 (MQ=255) tggtttCCAGCTGGAAGCTGGAAAGCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGAc < 2:219458/74‑1 (MQ=37) tggtttCCAGCTGGAAGCTGGAAAGCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGAc > 1:219458/4‑77 (MQ=37) tggtttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAg > 1:351021/4‑149 (MQ=255) cTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcac < 3:220191/78‑1 (MQ=255) cTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcac > 4:220191/1‑78 (MQ=255) tttCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtg > 6:246726/1‑149 (MQ=255) tttCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtg > 3:259299/1‑149 (MQ=255) tccagcttccagctGGAAACCACTGACAAGCTCAACTCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACATGGCCAAACACCGGATTTTCCGCGGTGACCGCCAggtggt > 7:237828/1‑149 (MQ=255) ccagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggt < 7:134769/148‑1 (MQ=255) ttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCaa > 7:173752/1‑149 (MQ=255) ccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCt < 2:253539/102‑1 (MQ=255) ccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTACTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCt > 1:253539/1‑102 (MQ=255) cagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACCg > 4:197565/1‑149 (MQ=255) | CAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCA‑CTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACCG > NC_002947/1521106‑1521396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |