Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,526,580 T→A 5.3% I256N (ATT→AAT)  ddlB → D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,526,5800TA 5.3% 229.3 / 2.7 75I256N (ATT→AAT) ddlBD‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base T (34/37);  new base A (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

CCGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAAC  >  NC_002947/1526432‑1526726
                                                                                                                                                     |                                                                                                                                                  
ccgccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAt                                                                                                                                                    >  8:79130/1‑149 (MQ=255)
 cgccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGTGATGCCAtt                                                                                                                                                   >  7:218455/1‑149 (MQ=255)
  gccgATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTTCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTg                                                                                                                                                  <  8:145973/149‑1 (MQ=255)
         gCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCAATGGCATCgc                                                                                                                                           <  2:334228/149‑2 (MQ=255)
           ccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAg                                                                                                                                         >  7:198079/1‑149 (MQ=255)
           ccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAg                                                                                                                                         >  3:143749/1‑149 (MQ=255)
              gggTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTCACCGCGCGC‑CCCTGCGATGCCATTGGCAACGAAGCCt                                                                                                                                      >  6:198913/1‑149 (MQ=255)
                    gccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGc                                                                                                                                >  1:232742/1‑149 (MQ=255)
                      cgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCgg                                                                                                                              <  4:194672/149‑1 (MQ=255)
                      cgcACGTGTTCTACGACTATGACGCCAAGTACATCGGCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCgg                                                                                                                              <  3:121095/149‑1 (MQ=255)
                        cACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGTTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                            <  2:359250/149‑1 (MQ=255)
                         aCGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTg                                                                                                                           >  8:118352/1‑149 (MQ=255)
                         aCGTGGTCTACGACTATGACGCCAAGTACATCGTCATCGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTg                                                                                                                           <  5:198913/149‑1 (MQ=255)
                           gtgtTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGa                                                                                                                         >  5:374/1‑149 (MQ=255)
                                  aCGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATg                                                                                                                  >  3:204667/1‑149 (MQ=255)
                                            cGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGc                                                                                                        <  7:118352/149‑1 (MQ=255)
                                              ccAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAg                                                                                                      <  7:79130/149‑1 (MQ=255)
                                               cAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGAACGAGCAgg                                                                                                     >  5:140861/1‑149 (MQ=255)
                                               cAAGTACATCGTCAACGATACCCAGTACCGCATCCCGAGCGGGCTGGATAGCGTCAACGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATAGGCATCCAACGCAGGGGCAGGTAGGACGTGATGCAGGACGAGCAgg                                                                                                     >  1:248635/1‑149 (MQ=255)
                                                  gTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGcc                                                                                                  <  4:159577/149‑1 (MQ=255)
                                                    aCATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAg                                                                                                               <  7:206473/134‑1 (MQ=255)
                                                    aCATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAg                                                                                                               >  8:206473/1‑134 (MQ=255)
                                                    aCATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTAACCGCCCCCAGCCTTCGATGCCAATGGCATCgca                                                                                                                                          <  3:287406/108‑3 (MQ=37)
                                                    aCATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTAACCGCCCCCAGCCTTCGATGCCAATGGCATCgca                                                                                                                                          >  4:287406/1‑106 (MQ=37)
                                                       tCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTc                                                                                             <  2:232742/149‑1 (MQ=255)
                                                        cGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGCCCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCt                                                                                            <  5:236281/149‑1 (MQ=255)
                                                          tCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTgg                                                                                          >  6:285594/1‑149 (MQ=255)
                                                           cAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgc                                                                                         >  1:172406/1‑149 (MQ=255)
                                                            aaCGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgct                                                                                        >  3:118975/1‑149 (MQ=255)
                                                              cGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgctgc                                                                                      <  4:143749/149‑1 (MQ=255)
                                                               gATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgctgct                                                                                     >  7:113375/1‑149 (MQ=255)
                                                                aTACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTc                                                                                    >  6:341442/1‑149 (MQ=255)
                                                                 tACCCAGTACCGCATCCCGTGCGGGCTGGATAGCCTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCg                                                                                   <  5:72409/149‑1 (MQ=255)
                                                                    ccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAg                                                                                >  3:51003/1‑149 (MQ=255)
                                                                    ccAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAg                                                                                >  3:90272/1‑149 (MQ=255)
                                                                     cAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGt                                                                               >  3:59957/1‑149 (MQ=255)
                                                                     cAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGAACGAGCAGGGCCGGTTCTGGCTGCTCGAAGt                                                                               <  6:140861/149‑1 (MQ=255)
                                                                      aGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTc                                                                              <  3:20520/149‑1 (MQ=255)
                                                                        tACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCaa                                                                            >  6:261264/1‑149 (MQ=255)
                                                                          ccGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAaca                                                                          <  5:341442/149‑1 (MQ=255)
                                                                           cGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAacac                                                                         >  5:240108/1‑149 (MQ=255)
                                                                            gCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCCACAcc                                                                        >  4:295711/1‑149 (MQ=255)
                                                                                  cGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATg                                                                                                                  <  5:60421/101‑1 (MQ=255)
                                                                                  cGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATg                                                                                                                  >  6:60421/1‑101 (MQ=255)
                                                                                   gTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAg                                                                 <  3:95734/149‑1 (MQ=255)
                                                                                     gCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGc                                                               <  8:113375/149‑1 (MQ=255)
                                                                                         gCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa                                                           >  5:10065/1‑149 (MQ=255)
                                                                                            ggATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGa                                                                                                                    >  8:214066/1‑89 (MQ=255)
                                                                                            ggATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGa                                                                                                                    <  7:214066/89‑1 (MQ=255)
                                                                                               tAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATc                                                     <  4:90272/149‑1 (MQ=255)
                                                                                                 gCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCAt                                                   <  8:198079/149‑1 (MQ=255)
                                                                                                  cGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATa                                                  <  5:261264/149‑1 (MQ=255)
                                                                                                       aggaacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTg                                             <  4:51003/149‑1 (MQ=255)
                                                                                                       aggaacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTg                                             >  4:317557/1‑149 (MQ=255)
                                                                                                        ggaacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTgg                                            >  7:39970/1‑149 (MQ=255)
                                                                                                         gaacaggaacTGATCGACCTGACCGCGCGC‑GGCTGCGAGGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGt                                           <  2:248635/149‑1 (MQ=255)
                                                                                                         gaacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGTGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATg                                                            <  6:124210/132‑1 (MQ=255)
                                                                                                         gaacaggaacTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATg                                                            >  5:124210/1‑132 (MQ=255)
                                                                                                                 acTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCAt                                                             <  2:108354/123‑1 (MQ=255)
                                                                                                                 acTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCAt                                                             >  1:108354/1‑123 (MQ=255)
                                                                                                                  cTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATggc                                  <  5:285594/149‑1 (MQ=255)
                                                                                                                     aTCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATggcggc                               >  6:88560/1‑149 (MQ=255)
                                                                                                                           cTGACCGCGCGC‑GCCTGTGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGc                         <  8:218455/149‑1 (MQ=255)
                                                                                                                                  cgcgc‑gcCTGCGATGCCATTGGCATCGAAGGCTGGGGGGGGTTGGACGTTATGCAGGACGAGCAGGGCCGTTTCTGGCTGCTCGAAGTCAACACGGCACCAGGCATTACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGc                  <  2:260779/149‑1 (MQ=255)
                                                                                                                                  cgcgc‑gcCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGc                  <  6:374/149‑1 (MQ=255)
                                                                                                                                   ccccagcCTTCGATGCCAATGGCATCGCAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGc                  >  8:121082/6‑149 (MQ=255)
                                                                                                                                            gCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTc         <  3:295711/149‑1 (MQ=255)
                                                                                                                                            gCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTc         >  3:87486/1‑149 (MQ=255)
                                                                                                                                            gCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTc         <  4:204667/149‑1 (MQ=255)
                                                                                                                                             cGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTcc        <  8:39970/149‑1 (MQ=255)
                                                                                                                                              gATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTc         >  2:225335/1‑147 (MQ=255)
                                                                                                                                                tGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAg      >  7:233501/1‑148 (MQ=255)
                                                                                                                                                  ccATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGc     <  4:87486/147‑1 (MQ=255)
                                                                                                                                                  ccATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCaa   >  8:148532/1‑149 (MQ=255)
                                                                                                                                                   cATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAAc  <  1:53348/149‑1 (MQ=255)
                                                                                                                                                    aTTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGc                                                <  7:313612/102‑1 (MQ=255)
                                                                                                                                                    aTTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGc                                                >  8:313612/1‑102 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCGCCGATCGCCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAGCAAC  >  NC_002947/1526432‑1526726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑