Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,757 A→T 10.0% intergenic (‑277/+36) galP‑II ← / ← ttgC porin‑like protein/efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,7570AT10.0% 108.6 / 3.9 40intergenic (‑277/+36)galP‑II/ttgCporin‑like protein/efflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base A (16/20);  new base T (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCAATTCGCCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCT  >  NC_002947/1576609‑1576896
                                                                                                                                                    |                                                                                                                                           
gCAATTCGCCGCAAACGGAACGGGGCGAT‑ATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGa                                                                                                                                             >  2:106039/1‑148 (MQ=255)
    ttCGCCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAg                                                                                                                                         <  3:236600/149‑1 (MQ=255)
        ccgcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTg                                                                                                                                     >  7:173984/1‑149 (MQ=255)
          gcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                                   >  5:210260/1‑149 (MQ=255)
           cAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGc                                                                                                                                  <  8:208818/149‑1 (MQ=255)
            aaaCGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCt                                                                                                                                 >  3:66202/1‑149 (MQ=255)
                  cacggGGCGATAATCGAACGGAATGGTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCGCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttgttt                                                                                                                           <  7:325028/148‑5 (MQ=38)
                      gggCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCtttt                                                                                                                       <  4:66202/149‑1 (MQ=255)
                        gCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGc                                                                                                                     <  5:286561/149‑1 (MQ=255)
                          gATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttg                                                                                                                              >  3:228781/1‑137 (MQ=255)
                          gATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttg                                                                                                                              <  4:228781/138‑2 (MQ=255)
                           aTAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGcaac                                                                                                                  <  6:210260/149‑1 (MQ=255)
                               tCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAgg                                                                                                              >  5:11203/1‑149 (MQ=255)
                                  aaCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGcc                                                                                                            >  3:234697/1‑148 (MQ=255)
                                           ttCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCtt                                                                                                  <  8:173984/149‑1 (MQ=255)
                                                gttggttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAATCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGgt                                                                                             <  1:120799/149‑1 (MQ=255)
                                                    gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCa                                                                                                       <  2:262777/135‑1 (MQ=255)
                                                    gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCa                                                                                                       >  1:262777/1‑135 (MQ=255)
                                                       aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                                   >  7:48150/1‑104 (MQ=255)
                                                       aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                                   <  8:48150/104‑1 (MQ=255)
                                                        ataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCa                                                                                      <  4:234697/148‑1 (MQ=255)
                                                                       cggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGtt                                                                      <  1:106039/149‑1 (MQ=255)
                                                                       cggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGACCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTCGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGtt                                                                      >  6:116413/1‑149 (MQ=255)
                                                                        ggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTg                                                                     <  6:11203/149‑1 (MQ=255)
                                                                                 gcgGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGc                                                                                                             >  7:101881/1‑100 (MQ=255)
                                                                                 gcgGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGc                                                                                                             <  8:101881/100‑1 (MQ=255)
                                                                                    ggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAg                                                                                                               >  7:335905/1‑95 (MQ=255)
                                                                                    ggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAg                                                                                                               <  8:335905/95‑1 (MQ=255)
                                                                                         aCCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTc                                                    >  5:263056/1‑149 (MQ=255)
                                                                                          cccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTACGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                                   >  7:105546/1‑149 (MQ=255)
                                                                                             gCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTAc                                                >  6:51997/1‑149 (MQ=255)
                                                                                                 cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAATGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGGCTGCTCGTACCAg                                             <  2:86600/148‑1 (MQ=255)
                                                                                                 cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCCGGTTGGCCTGCCCGGTCTGCTCGTACCa                                              <  7:238811/147‑1 (MQ=255)
                                                                                                               gcCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGcc                              >  2:162032/1‑149 (MQ=255)
                                                                                                                          acaGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGtt                   <  5:116413/149‑1 (MQ=255)
                                                                                                                                    aaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTg         <  6:263056/149‑1 (MQ=255)
                                                                                                                                         aaaGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAg    >  8:139574/1‑149 (MQ=255)
                                                                                                                                         aaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAg    >  8:133410/1‑149 (MQ=255)
                                                                                                                                           aGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCt  <  8:105546/149‑1 (MQ=255)
                                                                                                                                              ccACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGc           >  6:181112/1‑137 (MQ=255)
                                                                                                                                              ccACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGc           <  5:181112/137‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
GCAATTCGCCGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCT  >  NC_002947/1576609‑1576896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑