Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,594,073 G→A 7.6% intergenic (‑271/+30) PP_1399 ← / ← kgtP hypothetical protein/alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,594,0730GA 7.6% 190.0 / 4.5 66intergenic (‑271/+30)PP_1399/kgtPhypothetical protein/alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base A (3/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCT  >  NC_002947/1593925‑1594221
                                                                                                                                                    |                                                                                                                                                    
cgTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATggg                                                                                                                                                      <  5:120820/149‑1 (MQ=255)
        gTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCt                                                                                                                                              >  2:158828/1‑149 (MQ=255)
           cTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                                            <  3:38753/148‑1 (MQ=255)
            ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGcc                                                                                                                                                 <  6:133733/142‑1 (MQ=255)
            ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGcc                                                                                                                                                 >  5:133733/1‑142 (MQ=255)
              cAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttttcta                                                                                                                                        >  2:136018/1‑146 (MQ=39)
               aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttttcta                                                                                                                                        <  1:136018/148‑4 (MQ=39)
                ggCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATTGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                                            >  5:309741/1‑143 (MQ=255)
                ggCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                                            <  6:309741/143‑1 (MQ=255)
                 gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACt                                                                                                                                     >  6:295120/1‑149 (MQ=255)
                 gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACt                                                                                                                                     >  7:246513/1‑149 (MQ=255)
                 gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACt                                                                                                                                     >  2:96696/1‑149 (MQ=255)
                     cAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                                                   >  8:220586/1‑147 (MQ=255)
                       tggctggcagaacgttggcgcccgcgcgaacgctcccaagccagccATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccagg                                                                                                                               >  3:153280‑M2/47‑149 (MQ=255)
                             GCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATAAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGc                                                                                                                          >  2:344351‑M1/1‑148 (MQ=255)
                               ttGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGt                                                                                                                        >  7:245388/1‑148 (MQ=255)
                                     gCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAg                                                                                                                 <  5:295120/149‑1 (MQ=255)
                                      cGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGt                                                                                                                <  7:112685/149‑1 (MQ=255)
                                      cGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGt                                                                                                                <  7:220586/149‑1 (MQ=255)
                                       gTTCGCGCGGGCGCCAACGATCTGCCAGACATTGCAGCTTGATAAGGTGGAATTATTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCTCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                                               <  6:82339/149‑1 (MQ=255)
                                                     cAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGt                                                                                                  >  7:84319/1‑148 (MQ=255)
                                                            cTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTa                                                                                                                      >  7:324266/1‑121 (MQ=255)
                                                            cTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTa                                                                                                                      <  8:324266/121‑1 (MQ=255)
                                                             tGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTgg                                                                                         >  4:140746/1‑149 (MQ=255)
                                                                      ttGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCa                                                                                <  8:246513/149‑1 (MQ=255)
                                                                        gCATCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATATAAAAGGGGCGCCCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCAt                                                                               <  5:140873/148‑1 (MQ=255)
                                                                          aGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCa                                                                                <  3:325370/145‑1 (MQ=255)
                                                                          aGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCa                                                                                >  4:325370/1‑145 (MQ=255)
                                                                          aGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGt                                                                            >  6:357447/1‑149 (MQ=255)
                                                                           gCTTGATAAGGGGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTc                                                                           <  3:149317/149‑1 (MQ=255)
                                                                               gATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTcgcg                                                                        >  2:61138/1‑148 (MQ=255)
                                                                                 tAAGGTGGAAGTAATAGGGCGTTAAGAAGGAACAGGCTGAATAGAAACGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTaa                                                                     <  8:245388/149‑1 (MQ=255)
                                                                                        gAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACcag                                                                >  2:359715/1‑147 (MQ=255)
                                                                                         aaTTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACcagcag                                                             >  8:165587/1‑149 (MQ=255)
                                                                                          aTTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCagcagc                                                            <  4:153280/149‑1 (MQ=255)
                                                                                           ttAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCagcagc                                                            >  2:104963/1‑148 (MQ=255)
                                                                                             aaTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                                         >  2:176120/1‑149 (MQ=255)
                                                                                              aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                                         >  8:167473/1‑148 (MQ=255)
                                                                                                  ggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGc                                                    <  5:357447/149‑1 (MQ=255)
                                                                                                  ggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGc                                                    <  3:140746/149‑1 (MQ=255)
                                                                                                   gCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGc                                                    >  1:328670/1‑148 (MQ=255)
                                                                                                      ttAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATa                                                >  5:148663/1‑149 (MQ=255)
                                                                                                       tAAGAAGGAAAAGTCTTAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                                                  <  5:264751/146‑1 (MQ=255)
                                                                                                       tAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                                                  >  6:264751/1‑146 (MQ=255)
                                                                                                        aagaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATa                                                >  7:96616/1‑147 (MQ=255)
                                                                                                        aagaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATAc                                               <  2:328670/148‑1 (MQ=255)
                                                                                                          gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTaa                                                                     <  4:63957/124‑1 (MQ=255)
                                                                                                          gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTaa                                                                     >  3:63957/1‑124 (MQ=255)
                                                                                                           aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggc                                                                                                                              <  8:14245/66‑1 (MQ=255)
                                                                                                           aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggc                                                                                                                              >  7:14245/1‑66 (MQ=255)
                                                                                                           aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                                         <  4:141866/135‑1 (MQ=255)
                                                                                                           aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                                         >  3:141866/1‑135 (MQ=255)
                                                                                                           aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGc                                           >  2:158880/1‑149 (MQ=255)
                                                                                                                aaaGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGa                                      <  6:148663/149‑1 (MQ=255)
                                                                                                                  aGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACg                                    <  1:359715/149‑1 (MQ=255)
                                                                                                                           ctaaaaGGGGCGCCCCATTGCATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAg                              >  4:193018/3‑146 (MQ=39)
                                                                                                                            gAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                                 >  7:34554/1‑142 (MQ=255)
                                                                                                                            gAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                                 <  8:34554/142‑1 (MQ=255)
                                                                                                                                 ggggCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCtt                                                                                   >  7:98870/1‑87 (MQ=255)
                                                                                                                                 ggggCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCtt                                                                                   <  8:98870/87‑1 (MQ=255)
                                                                                                                                    gcgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctc                  >  6:310202/1‑149 (MQ=255)
                                                                                                                                     cgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGctct                 <  7:167473/149‑1 (MQ=255)
                                                                                                                                      gcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCtctc                <  1:158828/149‑1 (MQ=255)
                                                                                                                                           aTGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                                 <  1:108969/127‑1 (MQ=255)
                                                                                                                                           aTGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                                 >  2:108969/1‑127 (MQ=255)
                                                                                                                                               aaTGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGgc       >  2:29905/1‑149 (MQ=255)
                                                                                                                                                 tGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGgcgc     <  1:29905/149‑1 (MQ=255)
                                                                                                                                                    ggCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGctct  <  8:84319/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCT  >  NC_002947/1593925‑1594221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑