Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 162,270 C→T 7.8% intergenic (‑304/+70) PP_0153 ← / ← scpC hypothetical protein/propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947162,2700CT 7.8% 144.5 / 2.7 52intergenic (‑304/+70)PP_0153/scpChypothetical protein/propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTACTGGTCCGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGG  >  NC_002947/162124‑162412
                                                                                                                                                  |                                                                                                                                              
gTACTGGTCCGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCAAAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGTCGAAGTGATAGAAGGGTTTGACTGTTATGGCCCAt                                                                                                                                              >  4:256137/1‑149 (MQ=255)
      gTCCGCACTTATTTGGTGCTTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGGCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCgg                                                                                                                                        <  7:236794/149‑1 (MQ=255)
         cGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGc                                                                                                                                      >  1:2013/1‑148 (MQ=255)
           cACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAg                                                                                                                                   <  4:23558/149‑1 (MQ=255)
            aCTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGc                                                                                                                                  <  4:351132/149‑1 (MQ=255)
                      tGCGTTTTGATTCGAAGAATGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAagag                                                                                                                        <  8:261024/149‑1 (MQ=255)
                               aTTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGccataa                                                                                                                 >  7:111530/1‑143 (MQ=37)
                               aTTCGAACAAGGGCATTTGTCAGACGGGAAGGTCTGAAAGAACCAGAACAGTAGTACAGATTATGCTGCTAGTAGAGGGCTGACGTAGAGATAGAAGAGTTTGACTCTTATGGCCAAATCGACGTCAAGAAAGCGAAGAGGCCGGAAc                                                                                                                >  4:280895/1‑148 (MQ=255)
                                ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGCCATAACAg                                                                                                              <  8:111530/149‑1 (MQ=37)
                                ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAg                                                                                                              <  5:230632/149‑1 (MQ=255)
                                ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAACCCGCGAAGAGGCCGGAACAg                                                                                                              >  7:187287/1‑149 (MQ=255)
                                  cGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                             <  8:318093/148‑1 (MQ=255)
                                   gaagaaGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCTCGAAGAGGCCGGAACAGCg                                                                                                            >  8:237482/1‑148 (MQ=255)
                                    aagaagGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                             >  2:274637/1‑146 (MQ=255)
                                    aagaagGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGCTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                             <  1:274637/146‑1 (MQ=255)
                                         ggggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGaa                                                                                                         <  4:220414/145‑1 (MQ=255)
                                         ggggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAg                                                                                                     <  8:40429/149‑1 (MQ=255)
                                         ggggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTAGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGaa                                                                                                         >  3:220414/1‑145 (MQ=255)
                                                 gTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGccgccg                                                                                             >  1:114828/1‑149 (MQ=255)
                                                    aGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTc                                                                                          >  4:1651/1‑149 (MQ=255)
                                                       cGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCg                                                                                        <  3:280895/148‑1 (MQ=255)
                                                         ggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAAc                                                                                                                <  6:318825/122‑1 (MQ=255)
                                                         ggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAAc                                                                                                                >  5:318825/1‑122 (MQ=255)
                                                                   aaaGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTCCCCCATCCGGa                                                                           >  5:356779/1‑149 (MQ=255)
                                                                   aaaGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGa                                                                           >  5:40897/1‑149 (MQ=255)
                                                                           aGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCgg                                                                                                           <  6:5396/109‑1 (MQ=255)
                                                                           aGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCgg                                                                                                           >  5:5396/1‑109 (MQ=255)
                                                                                agtagtACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                             <  4:240330/102‑1 (MQ=255)
                                                                                     taCAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCTATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATgcg                                                         >  8:35648/1‑149 (MQ=255)
                                                                                              tgctgcTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttc                                                <  2:114828/149‑1 (MQ=255)
                                                                                                ctgctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAACCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttct                                               <  8:187287/148‑1 (MQ=255)
                                                                                                   ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCTATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCa                                           <  7:35648/149‑1 (MQ=255)
                                                                                                   ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttcttc                                             >  2:48293/1‑147 (MQ=255)
                                                                                                   ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGGttcgtc                                             >  2:317589/1‑147 (MQ=255)
                                                                                                   ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGATATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttcttc                                             <  5:313828/147‑1 (MQ=255)
                                                                                                         gagGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGtttt                                     >  6:243879/1‑149 (MQ=255)
                                                                                                            gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTcc                                            >  6:156654/1‑139 (MQ=255)
                                                                                                            gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTcc                                            <  5:156654/139‑1 (MQ=255)
                                                                                                            gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGt                                  <  7:237482/149‑1 (MQ=255)
                                                                                                            gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCAGGTTTCTTCCAGGTTTTAGt                                  >  3:24846/1‑149 (MQ=255)
                                                                                                              gTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAg                                                                                                     <  5:241367/80‑1 (MQ=255)
                                                                                                              gTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAg                                                                                                     >  6:241367/1‑80 (MQ=255)
                                                                                                                 gCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTc                                    >  7:241949/1‑142 (MQ=255)
                                                                                                                 gCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTc                                    <  8:241949/142‑1 (MQ=255)
                                                                                                                            gAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                  >  6:259621/1‑149 (MQ=255)
                                                                                                                             aaGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTcg                 >  4:61450/1‑149 (MQ=255)
                                                                                                                             aaGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCCGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTcg                 >  5:25843/1‑149 (MQ=255)
                                                                                                                                     cgacagtATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGAt         <  1:308037/143‑1 (MQ=255)
                                                                                                                                      aCTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAACCAGGGCCGCCGGTCCGTCCCACAGCCGGATCAGCTGGCCCGCATGCGGCGGGTGACTGCAAGGGTTACGGCCCAGCGACGGGCATAGCGCAGGagg        >  8:264581/1‑147 (MQ=255)
                                                                                                                                        tGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGAt         >  2:308037/1‑146 (MQ=255)
                                                                                                                                            aTGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCgg   <  3:256137/148‑1 (MQ=255)
                                                                                                                                             tGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCggg  <  5:259621/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GTACTGGTCCGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGG  >  NC_002947/162124‑162412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑