Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,598,834 C→G 8.9% intergenic (‑73/+58) dctB ← / ← bglX C4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,598,8340CG 8.9% 133.7 / 4.4 45intergenic (‑73/+58)dctB/bglXC4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base C (22/19);  new base G (2/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

GGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTGC  >  NC_002947/1598687‑1598979
                                                                                                                                                   |                                                                                                                                                 
ggCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTgca                                                                                                                                                  <  8:260229/149‑1 (MQ=255)
                 ccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                                 >  1:1544/1‑149 (MQ=255)
                 ccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                                 >  4:129365/1‑149 (MQ=255)
                  cAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGtt                                                                                                                                >  4:156013/1‑149 (MQ=255)
                  cAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCgggt                                                                                                                                <  3:22691/149‑3 (MQ=37)
                          tCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                        <  3:156013/149‑1 (MQ=255)
                            agcagGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGa                                                                                                                      <  6:334123/149‑1 (MQ=255)
                                          ccccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTATTACCCGCGAAAAGGCCGGAACAg                                                                                                        >  3:19619/1‑149 (MQ=255)
                                            ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggc                                                                                                      <  4:76447/149‑1 (MQ=255)
                                               gACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTTCTCTGCAGCACCTGTAGGCGCGGTGTTTCCCGCGAAAACGCCGGAACAggcggc                                                                                                   >  2:175921/1‑149 (MQ=255)
                                               gACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCg                                                                                                              <  4:287570/138‑1 (MQ=255)
                                               gACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCg                                                                                                              >  3:287570/1‑138 (MQ=255)
                                                        cgcgAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGTAACAGGCGGCCttttt                                                                                             <  6:166197/146‑1 (MQ=255)
                                                        cgcgAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCttttt                                                                                             <  6:160900/146‑1 (MQ=255)
                                                        cgcgAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCttttt                                                                                             >  5:166197/1‑146 (MQ=255)
                                                        cgcgAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAg                                                                                          >  5:160900/1‑149 (MQ=255)
                                                                 aaaaGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGTCCTGCCTCTTCGCGGGTAAACCCGCTCCTACATGTACTCTGCAGCACGTGTAGGCGAGGTTTTACCCTCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCaa                                                                                 <  6:180066/149‑1 (MQ=38)
                                                                     gggCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTc                                                                              >  6:316655/1‑148 (MQ=255)
                                                                         aTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGc                                                                         >  2:281727/1‑149 (MQ=255)
                                                                                  ggATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTctgctgct                                                                >  7:121863/1‑149 (MQ=255)
                                                                                    aTCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTctgctgctg                                                               >  5:180537/1‑148 (MQ=255)
                                                                                    aTCCGGGGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCtgctgctgc                                                              <  2:243019/149‑1 (MQ=255)
                                                                                       cGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCAc                                                            <  1:281727/148‑1 (MQ=255)
                                                                                          tggtggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGcc                                                        <  6:180537/149‑1 (MQ=255)
                                                                                          tggtggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGcc                                                        >  8:73431/1‑149 (MQ=255)
                                                                                                 aTGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAAt                                                 >  2:49865/1‑149 (MQ=255)
                                                                                                           ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAc                                       >  2:271634/1‑149 (MQ=255)
                                                                                                           cggcctTTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAc                                       >  1:159820/3‑149 (MQ=18)
                                                                                                                  ttCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGc                                                         <  4:221599/124‑1 (MQ=255)
                                                                                                                  ttCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGc                                                         >  3:221599/1‑124 (MQ=255)
                                                                                                                     gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                              <  1:175921/148‑1 (MQ=255)
                                                                                                                     gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCCGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                              >  2:308659/1‑148 (MQ=255)
                                                                                                                       gggTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGaa                           <  3:129365/149‑1 (MQ=255)
                                                                                                                              cccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCt                    <  1:49865/149‑1 (MQ=255)
                                                                                                                              cccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCt                    <  7:73431/149‑1 (MQ=255)
                                                                                                                              cccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTAAACTCCCCt                    >  2:77905/1‑149 (MQ=255)
                                                                                                                                    ccTACAGGTGCTGCAGAGTACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCt                    <  3:143260/143‑1 (MQ=38)
                                                                                                                                    ccTACAGGTGCTGCAGAGTACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCt                    >  4:143260/1‑143 (MQ=38)
                                                                                                                                    ccTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGAGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCATCCCTACCTGGACATTGAACTCCCCTGGTTCg              <  5:316655/149‑1 (MQ=255)
                                                                                                                                     cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCt                                     <  7:204660/125‑1 (MQ=255)
                                                                                                                                     cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCt                                     >  8:204660/1‑125 (MQ=255)
                                                                                                                                     cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCgg             >  3:348800/1‑149 (MQ=255)
                                                                                                                                          ggTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTAAACTCCCCTGGTTCGGCAAcc        <  1:77905/149‑1 (MQ=255)
                                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCaa                                                                            <  2:70028/80‑1 (MQ=255)
                                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCaa                                                                            >  1:70028/1‑80 (MQ=255)
                                                                                                                                                 tGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCCCCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGATGCTGCACAGCCTGGGAATCCAGCCCTACATGGACATTGAACACCCATGGTTCGGCAACCCGCtcc  >  1:311706/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTGC  >  NC_002947/1598687‑1598979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑