Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 163,714 G→A 7.1% G40G (GGC→GGT)  scpC ← propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947163,7140GA 7.1% 205.6 / 3.2 70G40G (GGC→GGT) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (29/36);  new base A (2/3);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CTGGAACGGCATGCGGCGGGCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGAAACTAC  >  NC_002947/163567‑163862
                                                                                                                                                   |                                                                                                                                                    
cTGGAACGGCATGCGGCGGGCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGc                                                                                                                                                     >  6:182851/1‑149 (MQ=255)
             cggcggGCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGa                                                                                                                                         <  6:61067/148‑1 (MQ=255)
                 gggCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGAGTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCg                                                                                                                                    >  8:18880/1‑149 (MQ=255)
                   gCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCt                                                                                                                                  <  7:93239/149‑1 (MQ=255)
                   gCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCACCCGCGCCGGCGAAGccaag                                                                                                                                  <  4:210521/149‑4 (MQ=255)
                   gCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCACCCGCGCCGGCGAAGccaag                                                                                                                                  <  6:32683/149‑4 (MQ=255)
                    ccAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTc                                                                                                                                 <  7:47568/149‑1 (MQ=255)
                     cAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGGTGCGCCGGCGCGGGTGAGGCCGCTCa                                                                                                                                >  2:122614/1‑149 (MQ=255)
                      aaCACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCAt                                                                                                                               >  3:116880/1‑149 (MQ=255)
                      aaCACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCAt                                                                                                                               <  1:197788/149‑1 (MQ=255)
                        cacccCGGCCTCGGTCAGTTGTTTGTCCAGGGCATTGCCCAGGCTGGCGCCGGTCATCAGGCGGATTTTCAGGGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGc                                                                                                                             <  1:122614/149‑1 (MQ=255)
                           acCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGa                                                                                                                          >  7:294204/1‑149 (MQ=255)
                           acCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGa                                                                                                                          >  5:79566/1‑149 (MQ=255)
                                   cGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATg                                                                                                                  <  8:187560/149‑1 (MQ=255)
                                        aGTTGTATGTCGAGGTCTTTGCCCAGGCTGTCGCCGGTCATCAGGCTGATTTTCTGTGGCGTTTGGTTGGGAGGTTCGGCCATTGCATGCGGTAGGGGCTTGGCTTGGCCGGGGCGGGTGAAGCCGCTCATGCCGACTGTCATGCCGTc                                                                                                             <  4:71001/149‑1 (MQ=255)
                                          ttgtttgtCCAGGTCATTGCACAGGCTGGCTCCGGTCATCAGGCTGATTTGCAGTGGCGATTGCTTGTCACGTTCGGCCAATGCATGCGTTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACATTCATGCAGTCCt                                                                                                           <  7:155079/149‑1 (MQ=255)
                                            gtttgtCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTc                                                                                                          >  5:134080/1‑148 (MQ=255)
                                                   cAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGa                                                                                                   <  4:116880/148‑1 (MQ=255)
                                                   cAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGAc                                                                                                  >  1:18433/1‑149 (MQ=255)
                                                    aGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGAc                                                                                                  >  1:338914/1‑148 (MQ=255)
                                                     ggTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGAc                                                                                                  >  5:178652/1‑147 (MQ=255)
                                                      gTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAg                                                                                               >  6:242579/1‑149 (MQ=255)
                                                         aTTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCg                                                                                             <  6:134080/148‑1 (MQ=255)
                                                            gCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCACCCGCGCCGGCGAAGccaa                                                                                                                                   >  2:105122/1‑105 (MQ=255)
                                                            gCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCACCCGCGCCGGCGAAGccaa                                                                                                                                   <  1:105122/107‑3 (MQ=255)
                                                             cccAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCg                                                                                             <  5:355854/144‑1 (MQ=255)
                                                             cccAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCg                                                                                             >  6:355854/1‑144 (MQ=255)
                                                               cAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGAc                                                                                                  <  3:228253/137‑1 (MQ=255)
                                                               cAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGAc                                                                                                  >  4:228253/1‑137 (MQ=255)
                                                                aGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGc                                                                                     <  5:124227/149‑1 (MQ=255)
                                                                 ggCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGcc                                                                                    <  2:338914/149‑1 (MQ=255)
                                                                     ggCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCa                                                                                 >  2:321763/1‑148 (MQ=255)
                                                                      gcgcCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCa                                                                                 <  8:56569/147‑1 (MQ=255)
                                                                          cGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCAtt                                                                           <  1:321763/149‑1 (MQ=255)
                                                                            gTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTAc                                                                         <  5:242579/149‑1 (MQ=255)
                                                                                        aTTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGc                                                               <  2:207386/147‑1 (MQ=255)
                                                                                        aTTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAg                                                             >  2:34225/1‑149 (MQ=255)
                                                                                          tttCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAgg                                                            >  1:316981/1‑148 (MQ=255)
                                                                                           ttCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAggg                                                           <  7:18880/148‑1 (MQ=255)
                                                                                           ttCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAggg                                                           <  6:178652/148‑1 (MQ=255)
                                                                                            tCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGgga                                                          <  5:182851/148‑1 (MQ=255)
                                                                                                  gCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCAc                                                                                >  5:118599/1‑120 (MQ=255)
                                                                                                  gCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCAc                                                                                <  6:118599/120‑1 (MQ=255)
                                                                                                             gCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGAt                                         >  3:96835/1‑148 (MQ=255)
                                                                                                               aCGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGggag                                                         <  1:41615/130‑1 (MQ=255)
                                                                                                               aCGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGggag                                                         >  2:41615/1‑130 (MQ=255)
                                                                                                                cGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACg                                     <  7:222837/149‑1 (MQ=255)
                                                                                                                  ttCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGt                                   <  2:18433/149‑1 (MQ=255)
                                                                                                                    cGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCTTGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTa                                  <  6:79566/148‑1 (MQ=255)
                                                                                                                     ggCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTAc                                 <  1:34225/148‑1 (MQ=255)
                                                                                                                      gCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACAt                               <  3:105579/149‑1 (MQ=255)
                                                                                                                      gCCAATGCATGCGGTACGACCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACAt                               <  4:235130/149‑1 (MQ=255)
                                                                                                                        cAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGAATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGTACAAGCGGATACGATCACGGTACATg                              >  5:254454/1‑148 (MQ=255)
                                                                                                                         aaTGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGCTCACGGTACATGGa                            >  4:272599/1‑149 (MQ=255)
                                                                                                                            gcatgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGc                                                  >  3:18306/1‑124 (MQ=255)
                                                                                                                            gcatgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGc                                                  <  4:18306/124‑1 (MQ=255)
                                                                                                                             catgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAATCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGt                        >  1:275589/1‑149 (MQ=255)
                                                                                                                              atgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCAt                                                                            <  6:209145/96‑1 (MQ=255)
                                                                                                                              atgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCAt                                                                            >  5:209145/1‑96 (MQ=255)
                                                                                                                              atgcGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGt                        >  4:305986/1‑148 (MQ=255)
                                                                                                                                   gTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTAtctc                  <  4:96835/149‑1 (MQ=255)
                                                                                                                                    tACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCg                 <  3:305986/149‑1 (MQ=255)
                                                                                                                                      cGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCgg                <  5:23839/148‑1 (MQ=255)
                                                                                                                                         gagcggCTTCACCCGCGCCGGCGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTa            >  6:68527/5‑149 (MQ=255)
                                                                                                                                              gCTTCGCCTGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACAt                               <  6:251805/125‑1 (MQ=255)
                                                                                                                                              gCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACAt                               >  5:251805/1‑125 (MQ=255)
                                                                                                                                               tttCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGaaa      <  7:261971/148‑1 (MQ=255)
                                                                                                                                                ttCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGAAac     >  8:150974/1‑149 (MQ=255)
                                                                                                                                                ttCGCCGGCGCGGGAGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGAAac     >  7:306900/1‑149 (MQ=255)
                                                                                                                                                 tCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGaaa      >  8:261971/1‑147 (MQ=255)
                                                                                                                                                   gCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGAAactac  >  5:63508/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGGAACGGCATGCGGCGGGCCAACACACCGGCCTCGGTCAGTTGTTTGTCCAGGTCATTGCCCAGGCTGGCGCCGGTCATCAGGCTGATTTTCAGTGGCGATTGCTTGGCACGTTCGGCCAATGCATGCGGTACGGCCTTGGCTTCGCCGGCGCGGGTGAAGCCGCTCATGCCGACAGTCATGCCGTCCTCGATCAGACCAGCGGCATCAGCCGCACTCATTACCTTGCTGTGCAGGGAGGACAAGCGGATACGATCACGGTACATGGATTGTTATCTCGGGCTACTGAAACTAC  >  NC_002947/163567‑163862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑