| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,642,271 | G→C | 8.8% | intergenic (‑99/+53) | PP_1442 ← / ← lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,642,271 | 0 | G | C | 8.8% | 164.8 / 4.4 | 54 | intergenic (‑99/+53) | PP_1442/lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Reads supporting (aligned to +/- strand): ref base G (21/28); new base C (2/3); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
GCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGC > NC_002947/1642123‑1642415 | gCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAg < 7:234330/149‑1 (MQ=255) cAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAgg > 8:268470/1‑149 (MQ=255) cGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTAccc < 5:89550/149‑1 (MQ=255) cTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGGGCAAGCCCGCACCTGCACGGGTAC‑GCTACCCCTG‑Ca < 6:138416/149‑1 (MQ=38) tCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGCGTAG‑CGTACCCGTG‑CAg > 7:338810/1‑149 (MQ=255) ggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCg < 2:228085/149‑1 (MQ=255) gAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCgg < 4:338877/149‑1 (MQ=255) gAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTAC‑GCTACCCCTG‑CAGGCGCgg < 1:44570/149‑1 (MQ=18) aCGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTAC‑GCTACCCCTG‑CAGGCGCGGGc < 6:113990/149‑1 (MQ=18) gAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCtt > 8:97546/1‑149 (MQ=255) aGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGATGCCTGTGCCGGTCCTTTCGCGAGTAAGCCAGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTg > 7:149891/1‑149 (MQ=255) gCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGc > 2:98520/1‑149 (MQ=255) aGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGccc > 4:46364/1‑149 (MQ=255) ggTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGc < 7:268470/149‑1 (MQ=255) ggTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATAACCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGc < 5:123490/149‑1 (MQ=255) aTGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGt < 3:46364/148‑1 (MQ=255) gtcgCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGCACGAGGAATGTTGTGCTGCCTGTGCCGGTCCTTTCGCGCGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGAG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAg < 4:241762/146‑1 (MQ=255) tggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTATCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg < 5:23334/149‑1 (MQ=255) tCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGc > 2:74765/1‑146 (MQ=255) aGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTGCCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa < 1:74765/149‑1 (MQ=255) aGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAg < 7:97546/149‑1 (MQ=255) cTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGTCGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCa < 1:98520/149‑1 (MQ=255) gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTA‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCg > 3:303223/1‑149 (MQ=255) tGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGcc < 2:66253/149‑1 (MQ=255) tGGCCTGAGGAAACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGc > 6:288992/1‑148 (MQ=255) ccTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACg > 6:3709/1‑149 (MQ=255) cTGAGGATACCCGATGATCGAGGAATTTTGTGCTGCCTGTGCGGGTCCTTTCGCTAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGGACTTTCGCCACGt < 8:241592/149‑1 (MQ=255) ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAgcg > 3:316835/1‑149 (MQ=255) atgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCtt < 5:337883/149‑1 (MQ=255) gatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTg < 4:2553/148‑1 (MQ=255) tCGAGGATATTTGTGATTCCTGTGCGGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc < 8:149891/148‑1 (MQ=255) cGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCg > 7:129385/1‑148 (MQ=255) cGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCg > 1:57970/1‑148 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc < 4:278310/146‑1 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGc > 3:278310/1‑146 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGa > 2:335448/1‑148 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTA‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTg < 8:68449/103‑1 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTA‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTg > 7:68449/1‑103 (MQ=255) ggAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAAt < 2:57970/149‑1 (MQ=255) aaTTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGc < 4:316835/148‑1 (MQ=255) tgctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTT‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTc < 4:303223/148‑1 (MQ=255) gctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTg > 2:256589/1‑91 (MQ=255) gctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGCTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTg < 1:256589/91‑1 (MQ=255) tgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGc > 6:356997/1‑149 (MQ=255) cTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGGAAAGCCCGTGGGGAAATGCACTGTCAGGCCtt > 2:258293/1‑149 (MQ=255) gAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTa > 6:217988/1‑141 (MQ=255) gAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTa < 5:217988/141‑1 (MQ=255) aGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttct < 5:3709/149‑1 (MQ=255) gCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑TAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt > 1:215627/1‑149 (MQ=255) gCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt > 5:57748/1‑149 (MQ=255) gCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt < 1:258293/149‑1 (MQ=255) cccGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc < 3:195325/149‑1 (MQ=255) ccGCACCTGCACGGGTAC‑GCTACCCCTG‑CAGGCGCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc > 2:118859/1‑148 (MQ=37) gcgcCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa < 1:335448/149‑1 (MQ=255) gcgcCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa < 5:356997/149‑1 (MQ=255) tGCACGGGTAA‑GCTACCCCTG‑CAGGTGCGGGCTTGAGCGCGAAGAGGGCGCTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTcgc > 8:44356/1‑149 (MQ=255) | GCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAG‑CGTACCCGTG‑CAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAGTCGC > NC_002947/1642123‑1642415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |