Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,708,163 G→C 7.5% intergenic (‑48/+3) PP_1502 ← / ← PP_1503 OmpA family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,708,1630GC 7.5% 187.2 / 4.4 63intergenic (‑48/+3)PP_1502/PP_1503OmpA family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCTTGGCCT  >  NC_002947/1708017‑1708311
                                                                                                                                                  |                                                                                                                                                    
ggTGCGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAggg                                                                                                                                                     <  4:313325/148‑1 (MQ=255)
 gagCGGGCCGATTCCAGGTTCGCCTGCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCt                                                                                                                                                   <  8:229566/147‑1 (MQ=255)
 gTGCGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCt                                                                                                                                                   <  7:28044/149‑1 (MQ=255)
   gCGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCa                                                                                                                                                 >  2:208373/1‑149 (MQ=255)
    cGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCa                                                                                                                                                 <  4:325407/148‑1 (MQ=255)
     gggCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACt                                                                                                                                               >  8:216658/1‑149 (MQ=255)
         cGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAgc                                                                                                                                           <  3:294292/149‑1 (MQ=255)
          gATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAgcg                                                                                                                                          >  1:95482/1‑149 (MQ=255)
            ttCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCt                                                                                                                                        <  4:162888/149‑1 (MQ=255)
               cAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTTTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGCGCTCAGTGAGCCCTgcc                                                                                                                                     <  8:286503/149‑2 (MQ=255)
               cAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGCGCTCAGTGAGCCCTgcc                                                                                                                                     <  3:340792/149‑2 (MQ=255)
                      gCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGAGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCGTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCCCTGAGCGCTGCGCAGGCCt                                                                                                                              >  4:32482/1‑149 (MQ=255)
                        aTTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTc                                                                                                                            <  6:266928/149‑1 (MQ=255)
                                  tGCGAGCAGGCAGCAAGACCGCCGCTCAGGCCCAGCAGGGCGGGAATCATTGCGTGTTTGCGCATAGTGTTCATCGTTTGATCGTGTGCGAAGGCTACGGTTGGCGGGGCAGCGCTCACTGAGCGCTGCGCAGGCCTTCCTCacggacg                                                                                                                  >  4:13564/1‑149 (MQ=255)
                                  tGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCacgtacg                                                                                                                  >  6:21137/1‑149 (MQ=255)
                                     gAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTc                                                                                                                <  1:87463/148‑1 (MQ=255)
                                     gAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTc                                                                                                                >  2:87463/1‑148 (MQ=255)
                                       gcaggcagCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCt                                                                                                              >  8:55263/1‑148 (MQ=255)
                                         aggcagCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGaa                                                                                                           >  3:318631/1‑149 (MQ=255)
                                          ggcagCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAAc                                                                                                          <  4:318631/149‑1 (MQ=255)
                                           gcagcaAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACg                                                                                                         >  1:66598/1‑149 (MQ=255)
                                              tcaAGACCGACGCTCAGTGCCAGCAGGTCGTTAATCATTACGTGTTTGCGAATAGTTTTCATCCTTTGATCGTGTTCGAAGGCTAAGGTTGGAGGGGCAGTGCTCACTGAGCGTTGCGCAGGCCTTCCTCACGTACGTCCTGAACGccc                                                                                                      <  1:356764/148‑1 (MQ=255)
                                                aaGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTg                                                                                                    >  2:185353/1‑149 (MQ=255)
                                                aaGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTg                                                                                                    >  7:136278/1‑149 (MQ=255)
                                                aaGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTg                                                                                                    >  4:312367/1‑149 (MQ=255)
                                                aaGACCGACGCTCAGGGCCAGCAGGGCGGGAATCAGTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGGTGGCGGGGCAGGTCTCCCTGAGCGCTGCGCAGGCCTTCCTCACGTCCGGCCTGAACGCCCTg                                                                                                    >  4:95861/1‑149 (MQ=255)
                                                     cGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCggg                                                                                               >  8:256671/1‑149 (MQ=255)
                                                     cGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCggg                                                                                               >  3:49019/1‑149 (MQ=255)
                                                         gCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATc                                                                                           <  7:216658/149‑1 (MQ=255)
                                                               ggCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCacgtacgt                                                                                                                 <  4:96502/121‑1 (MQ=255)
                                                               ggCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCacgtacgt                                                                                                                 >  3:96502/1‑121 (MQ=255)
                                                               ggCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCAc                                                                                     >  1:134191/1‑149 (MQ=255)
                                                                        ggCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTg                                                                            <  2:66598/149‑1 (MQ=255)
                                                                          cGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTggg                                                                          >  7:76158/1‑149 (MQ=255)
                                                                          cGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTggg                                                                          <  4:325476/149‑1 (MQ=255)
                                                                            ggAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGCGCTCAGTGAGCCCTgccc                                                                                                                                    <  6:192202/89‑3 (MQ=39)
                                                                            ggAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGCGCTCAGTGAGCCCTgccc                                                                                                                                    >  5:192202/1‑87 (MQ=39)
                                                                                        tgtTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTgg                                                            <  4:49019/149‑1 (MQ=255)
                                                                                          tttGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTggg                                                           <  1:208373/148‑1 (MQ=255)
                                                                                               gcATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGGTTTGGCTGCCTGGGCCTTGc                                                     <  2:95482/149‑1 (MQ=255)
                                                                                                     tgtTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCgg                                               >  7:333669/1‑149 (MQ=255)
                                                                                                         cATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAg                                           >  3:34731/1‑149 (MQ=255)
                                                                                                           tCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGc                                         >  1:171923/1‑149 (MQ=255)
                                                                                                             cTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGt                                       <  2:134191/149‑1 (MQ=255)
                                                                                                                tGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGc                                     <  7:256671/148‑1 (MQ=255)
                                                                                                                  aTCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGc                                                                                                  >  4:336531/1‑85 (MQ=255)
                                                                                                                  aTCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGc                                                                                                  <  3:336531/85‑1 (MQ=255)
                                                                                                                        tgCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGTTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTc                            <  8:136278/149‑1 (MQ=255)
                                                                                                                          cGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCgg                          >  8:304425/1‑149 (MQ=255)
                                                                                                                           gAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGa                                            <  2:33348/130‑1 (MQ=255)
                                                                                                                           gAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGa                                            >  1:33348/1‑130 (MQ=255)
                                                                                                                           gAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGc                         <  3:312367/149‑1 (MQ=255)
                                                                                                                           gAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGc                         <  1:185353/149‑1 (MQ=255)
                                                                                                                            aaGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGcc                        >  1:73645/1‑149 (MQ=255)
                                                                                                                               gCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGTCCTTCTGGGCTCTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGt                     >  7:190621/1‑149 (MQ=255)
                                                                                                                                cTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGtt                    >  2:47439/1‑149 (MQ=255)
                                                                                                                                   aGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTg                   <  8:333669/147‑1 (MQ=255)
                                                                                                                                    gggtGGCGGGGCAGGGCTCACTGACCGCGGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCCTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGc                <  8:190621/146‑1 (MQ=255)
                                                                                                                                    ggTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGc                >  2:35533/1‑149 (MQ=255)
                                                                                                                                      ttGGCGGGGCAGGGCTCACTGAGCGATGCGCAGGCCTTCCTCACGTCCGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGGGCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCa              <  3:95861/149‑1 (MQ=255)
                                                                                                                                             ggCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCtt       <  3:32482/149‑1 (MQ=255)
                                                                                                                                              gCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCTTg      >  1:235701/1‑149 (MQ=255)
                                                                                                                                              gCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCTTg      <  2:171923/149‑1 (MQ=255)
                                                                                                                                              gCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCTTg      <  2:73645/149‑1 (MQ=255)
                                                                                                                                                  ggCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCGGGGCCTTGCGTTCGGCGAGGCGTGCAGCCCACGCGGCCTGTTGGGCCCGTCGCTTGGCCt  >  1:288882/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGTGCGGGCCGATTCCAGGTTGGCATTCGGATCATGCGAGCAGGCAGCAAGACCGACGCTCAGGGCCAGCAGGGCGGGAATCATTACGTGTTTGCGCATAGTGTTCATCCTTTGATCGTGTGCGAAGGCTAAGGTTGGCGGGGCAGGGCTCACTGAGCGCTGCGCAGGCCTTCCTCACGTACGTCCTGAACGCCCTGGCGGGCATCCTGCACGGCCTTCTGGGCTTTGGCTGCCTGGGCCTTGCGTTCGGCGAGGCGTGCATCCCACTCGGCCTGTTGGGCCAGTCGCTTGGCCT  >  NC_002947/1708017‑1708311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑