Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,717,899 G→T 7.7% V200L (GTG→TTG)  rsmJ → ribosomal RNA small subunit methyltransferase J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,717,8990GT 7.7% 162.0 / 3.0 52V200L (GTG→TTG) rsmJribosomal RNA small subunit methyltransferase J
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCGCATGCGCCTGCTGACCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGA  >  NC_002947/1717751‑1718047
                                                                                                                                                    |                                                                                                                                                    
gcgcATGCGCCTGCTGACCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTtgg                                                                                                                                                      <  4:137767/149‑1 (MQ=255)
        gcCTGCTGACCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGAt                                                                                                                                              >  3:216954/1‑149 (MQ=255)
             ctgACCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGCTGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCt                                                                                                                                         <  6:347107/149‑1 (MQ=255)
                aCCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTgg                                                                                                                                       <  7:89760/148‑1 (MQ=255)
                  cGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATg                                                                                                                                    >  3:161869/1‑149 (MQ=255)
                   ggCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGc                                                                                                                                   >  5:272155/1‑149 (MQ=255)
                                      tGCGCGCCTGGGAATGGGAGGCGGCGAAGGTGTTATACCTCGACCCGATGTTTCCACACCGCGAGAAGTGCGGCCTGGTTAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGGGATGACCTGGATGGCCCTGCGCTGCTCgta                                                                                                                   <  8:239623/146‑3 (MQ=255)
                                      tGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGcc                                                                                                                >  8:103774/1‑149 (MQ=255)
                                      tGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCACCGGAGCTGCTCGaa                                                                                                                   >  7:239623/1‑146 (MQ=255)
                                         gcgcCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGct                                                                                                             >  5:98484/1‑149 (MQ=255)
                                                ggAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGgc                                                                                                        <  7:103774/147‑1 (MQ=255)
                                                  aaGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACACGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  5:289402/149‑1 (MQ=255)
                                                  aaGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  4:161869/149‑1 (MQ=255)
                                                  aaGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  2:82906/149‑1 (MQ=255)
                                                            gccgcAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGgcca                                                                                                >  7:28105/1‑143 (MQ=255)
                                                            gccgcAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGgcca                                                                                                <  8:28105/143‑1 (MQ=255)
                                                                        cTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGTTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    >  7:222864/1‑127 (MQ=255)
                                                                        cTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGTTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    >  7:217383/1‑127 (MQ=255)
                                                                        cTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGTTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  8:217383/127‑1 (MQ=255)
                                                                        cTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGTTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  8:222864/127‑1 (MQ=255)
                                                                         tACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTg                                                                               >  6:270059/1‑147 (MQ=255)
                                                                           ccTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGaa                                                                             <  4:172703/147‑1 (MQ=255)
                                                                           ccTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAgc                                                                           >  6:113569/1‑149 (MQ=255)
                                                                             tCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGaa                                                                             <  4:69175/145‑1 (MQ=255)
                                                                             tCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGaa                                                                             >  3:69175/1‑145 (MQ=255)
                                                                               gACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAgcgc                                                                         <  5:113569/147‑1 (MQ=255)
                                                                                 cccGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTa                                                                                  <  2:22627/136‑1 (MQ=255)
                                                                                 cccGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTa                                                                                  >  1:22627/1‑136 (MQ=255)
                                                                                     aTGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTgg                                                                                                   <  2:262359/115‑1 (MQ=255)
                                                                                     aTGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGgccagc                                                                                              >  2:176289/1‑120 (MQ=255)
                                                                                     aTGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGgccagc                                                                                              <  1:176289/120‑1 (MQ=255)
                                                                                       gTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTg                                                                                                    <  8:294083/112‑1 (MQ=255)
                                                                                         ttCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCa                                                             >  8:42869/1‑149 (MQ=255)
                                                                                          tCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACg                                                                    <  4:4965/141‑1 (MQ=255)
                                                                                          tCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACg                                                                    >  3:4965/1‑141 (MQ=255)
                                                                                          tCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCAGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCGACCGGGTGGTAGTGAAGCGCGCACGCACGGCag                                                            >  8:336128/1‑148 (MQ=255)
                                                                                           ccACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCAcc                                                           >  8:299897/1‑149 (MQ=255)
                                                                                            cacaCCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCAcc                                                           <  5:270059/148‑1 (MQ=255)
                                                                                             acacCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGa                                                         <  7:299897/149‑1 (MQ=255)
                                                                                              cacCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCGCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGat                                                        <  4:216954/149‑1 (MQ=255)
                                                                                                ccGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGatca                                                      >  2:218809/1‑149 (MQ=255)
                                                                                                    gACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGACCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGa                                                  >  1:76282/1‑149 (MQ=255)
                                                                                                       aaGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACgg                                               <  2:76282/149‑1 (MQ=255)
                                                                                                                        aaaGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCa                                >  3:134623/1‑147 (MQ=255)
                                                                                                                              aaTGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGaa                                                                             >  6:279794/1‑96 (MQ=255)
                                                                                                                              aaTGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGaa                                                                             <  5:279794/96‑1 (MQ=255)
                                                                                                                                        ttCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGt                   >  5:41245/1‑144 (MQ=255)
                                                                                                                                        ttCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGt                   <  6:41245/144‑1 (MQ=255)
                                                                                                                                        ttCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTc              >  5:366958/1‑149 (MQ=255)
                                                                                                                                             gCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTaa                 <  3:148104/141‑1 (MQ=255)
                                                                                                                                             gCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTaa                 >  4:148104/1‑141 (MQ=255)
                                                                                                                                             gCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGACTTGAGGGTAAGTCGAGcc         >  5:109807/1‑149 (MQ=255)
                                                                                                                                              ccTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCg        >  3:143358/1‑149 (MQ=255)
                                                                                                                                                  tggtggGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTa     <  7:42869/148‑1 (MQ=255)
                                                                                                                                                    gtggGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGa  >  6:143312/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCATGCGCCTGCTGACCGGCAACGCCATCGAGCGCATGCGCGCCTGGGAAGGGGAGGCGCCGCAGGTGATCTACCTCGACCCGATGTTTCCACACCGCGACAAGAGCGCCCTGGTGAAAAAGGAAATGCGCGTGTTCCGGCCTTTGGTGGGCGATGACCTGGATGCCCCGGCGCTGCTCGAAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGA  >  NC_002947/1717751‑1718047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑