Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,728,864	G→C	7.9%	A81A (GCC→GCG)	PP_1523 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,728,864	0	G	C	7.9%	181.4 / 5.1	61	A81A (GCC→GCG)	PP_1523	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base C (2/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

CTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTCCACTCGG  >  NC_002947/1728721‑1729012                                                                                                                                                |                                                                                                                                                        cTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGcgc‑g                                                                                                                                                   <  6:124348/149‑1 (MQ=255)     cgggtCATGAGGCACGCCAGGGGCCTTCCTCTTCACCGTCGGCGCTCATGCGGACCCAGCGGGCAGCATCTTCTTCCCCGGCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCCGCGCCGCATTTGCCGCGC‑tgggt                                                                                                                                               <  8:335580/145‑3 (MQ=255)            tgatgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCaa                                                                                                                                        >  8:15997/1‑149 (MQ=255)             gatgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑c                                                                                                                                      >  4:98979/1‑149 (MQ=255)             gatgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑c                                                                                                                                      >  4:317309/1‑149 (MQ=255)              atgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGGGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑Cg                                                                                                                                     <  1:80724/149‑1 (MQ=255)              atgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑Cg                                                                                                                                     >  6:120576/1‑149 (MQ=255)               tgTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGt                                                                                                                                    <  3:317309/149‑1 (MQ=255)                gTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGt                                                                                                                                    >  1:318873/1‑148 (MQ=255)                gTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGt                                                                                                                                    >  8:219861/1‑148 (MQ=255)                gTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACATCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGt                                                                                                                                    >  7:244347/1‑148 (MQ=255)                   cGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCAt                                                                                                                                 <  2:318873/148‑1 (MQ=255)                         gTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCCGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCa                                                                                                                          >  4:292712/1‑149 (MQ=255)                          tGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCAc                                                                                                                         >  3:190118/1‑149 (MQ=255)                          tGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCAc                                                                                                                         >  3:194716/1‑149 (MQ=255)                           gATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCCCTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCCCg                                                                                                                        >  3:267392/1‑149 (MQ=255)                                    ttCAACGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCCCGGCGTCTGGt                                                                                                               >  7:235331/1‑149 (MQ=255)                                      cACCGGCGGCGCTGAGGCGGACCCCGCGGTCAGCATCTTCTTCCCCGTCTTCCTCCCCCCCGCCCCCCGGCGCGCCGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTgg                                                                                                                <  5:216399/146‑1 (MQ=255)                                       aCCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCg                                                                                                             <  3:98979/148‑1 (MQ=255)                                          gTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCttt                                                                                                         >  7:303886/1‑149 (MQ=255)                                             ggCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCa                                                                                                      <  8:167627/149‑1 (MQ=255)                                                        ggACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTc                                                                                                                   <  3:113418/125‑1 (MQ=255)                                                            ccAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCCGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATCCGGCGC‑GGGCGCAGGCAA‑CGACATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGCCTGtac                                                                                        >  7:327152/1‑146 (MQ=255)                                                              aGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCAt                                                                                     <  3:292712/149‑1 (MQ=255)                                                                  gTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                                                                                 <  7:219861/149‑1 (MQ=255)                                                                       cATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTa                                                                              >  7:120919/1‑147 (MQ=255)                                                                                ccccGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                                                                                 >  7:58240/1‑135 (MQ=255)                                                                                ccccGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTcc                                                                                 <  8:58240/135‑1 (MQ=255)                                                                                ccccGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTgg                                                                     <  7:155994/147‑1 (MQ=255)                                                                                  ccGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTaa                                                                 <  5:120576/149‑1 (MQ=255)                                                                                       ttCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAg                                                            >  2:82480/1‑149 (MQ=255)                                                                                          cTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCtt                                                                       >  3:33047/1‑135 (MQ=255)                                                                                          cTCGACCCAGCATCCAGGCGCGCAGCGCACTTCCTCACCCAGCGCGGCAAATGCGGCGC‑TGGAGCAGGCAA‑CGTAATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGTCTGTTCCATTTCCCTACAGCCtt                                                                       <  4:33047/135‑1 (MQ=255)                                                                                           tCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGAc                                                        >  3:249469/1‑149 (MQ=255)                                                                                                ccAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGaa                                                   >  5:233713/1‑149 (MQ=255)                                                                                                  aGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  <  1:165336/148‑1 (MQ=255)                                                                                                  aGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  >  2:165336/1‑148 (MQ=255)                                                                                                  aGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATg                                                 >  7:272897/1‑149 (MQ=255)                                                                                                      aCCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTAc                                             >  1:52578/1‑149 (MQ=255)                                                                                                       cccGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTAcc                                            <  1:82480/149‑1 (MQ=255)                                                                                                           gcgcgcAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGcc                                        >  2:57666/1‑149 (MQ=255)                                                                                                            cgcgcAGCGCTCTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCt                                       <  1:57666/149‑1 (MQ=255)                                                                                                             gcgcagcgcaCTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAc                                                                <  8:158431/123‑1 (MQ=255)                                                                                                             gcgcagcgcaCTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAAc                                                                >  7:158431/1‑123 (MQ=255)                                                                                                               gctccgcaCTTCCACTCCCAGCGCGGCAATTGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCGCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTGCATTTCCCTACAGCCTTGGGTAACCAAGTGTCCGGAATGTTACCGGCCTGGc                                    <  8:327152/145‑1 (MQ=255)                                                                                                                      aCTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGaa                            <  8:303886/149‑1 (MQ=255)                                                                                                                       cTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAg                           <  4:249469/149‑1 (MQ=255)                                                                                                                              acCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGTCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGc                    <  8:120919/149‑1 (MQ=255)                                                                                                                               gcccgcgcCGCATTTGCCGCGCTGGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  >  3:121111/5‑120 (MQ=255)                                                                                                                               gcccgcgcCGCATTTGCCGCGCTGGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  >  3:284781/5‑120 (MQ=255)                                                                                                                               gcccgcgcCGCATTTGCCGCGCTGGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  <  4:121111/116‑1 (MQ=255)                                                                                                                               gcccgcgcCGCATTTGCCGCGCTGGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAAt                                                  <  4:284781/116‑1 (MQ=255)                                                                                                                                    cgcgGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCt              <  6:73156/149‑1 (MQ=255)                                                                                                                                      cgGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGc            >  8:103373/1‑149 (MQ=255)                                                                                                                                      cgGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGc            <  7:103373/149‑1 (MQ=255)                                                                                                                                       ggCAAATGCGGCGC‑GGGCGCAGGCAAGCGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTGCATTTCCCTACAGCCTTGGGAAACCAAGTGCCCGTAAAGTAACC‑GCCGGGCAATTTGAAGGTAGGGCGttagtgc            >  1:3448/1‑144 (MQ=255)                                                                                                                                         cAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTc         >  6:351305/1‑149 (MQ=255)                                                                                                                                          aaaTGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGa                             >  7:63683/1‑128 (MQ=255)                                                                                                                                          aaaTGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGa                             <  8:63683/128‑1 (MQ=255)                                                                                                                                             tGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTCCACt     <  4:190118/149‑1 (MQ=255)                                                                                                                                             tGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTCCACt     <  4:194716/149‑1 (MQ=255)                                                                                                                                                ggcgC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTCCACTCgg  >  8:222844/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                        CTGCCGTTTCATGATGTACGCCAGGTGATTTCCTCTTCACCGTCGGCGCTGATGCGGACCCAGCGGTCAGCATCTTCTTCCCCGTCTTCCTCGACCCAGCCACCCGGCGCGCAGCGCACTTCCACACCCAGCGCGGCAAATGCGGCGC‑GGGCGCAGGCAA‑CGTCATCGGCCCACGGCGTCTGGTCGCTTTCCAGATACAGGCTGTTCCATTTCCCTACAGCCTTGGGTAACCAAGTGACCGGAATGTTACCGGCCTGGC‑ATTTGAAGGTATGGCCTTTCTGCTTCCACTCGG  >  NC_002947/1728721‑1729012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑