Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,747,863 C→T 9.2% intergenic (‑355/+176) PP_5473 ← / ← PP_5474 LuxR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,747,8630CT 9.2% 115.0 / 2.3 43intergenic (‑355/+176)PP_5473/PP_5474LuxR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/20);  new base T (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCTACCTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATGGGGAGCTAGGTGTA  >  NC_002947/1747717‑1748008
                                                                                                                                                  |                                                                                                                                                 
cGTCTACCTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCgg                                                                                                                                                 <  6:277726/149‑1 (MQ=255)
  tCTACCTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCgggg                                                                                                                                               >  6:106950/1‑149 (MQ=255)
      ccTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAt                                                                                                                                           <  4:126944/149‑1 (MQ=255)
       cTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAtt                                                                                                                                          >  3:124249/1‑149 (MQ=255)
        tCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCTGGGGTCAttt                                                                                                                                         >  7:304039/1‑149 (MQ=255)
        tCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCTGGGGTCAttt                                                                                                                                         >  7:299099/1‑149 (MQ=255)
        tCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAttt                                                                                                                                         >  2:74214/1‑149 (MQ=255)
        tCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAttt                                                                                                                                         >  5:358182/1‑149 (MQ=255)
        tCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAttt                                                                                                                                         >  1:319347/1‑149 (MQ=255)
         ccccGACCAACGGGAGGGCGAACGCCACGACCGATGCAGTCGGGTTAAGGTTGATGGGGCTGGGCCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAtttt                                                                                                                                        <  4:247360/149‑1 (MQ=255)
          ccGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCAgtttt                                                                                                                                       >  6:288149/1‑149 (MQ=255)
                cAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCt                                                                                                                                 <  5:106950/149‑1 (MQ=255)
                 aaGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTc                                                                                                                                <  2:319347/149‑1 (MQ=255)
                         ggagAACGTCACGACCGATGCCTTCGTTTTACGGTTGATGGGGTAGGGTCTTAGGGTCTGTACGCCGCACGGGCTAGCACCTTAAGGGCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCTGGGGTCATTTTTTCGGCTCAGCTTTAc                                                                                                                        <  8:304039/146‑1 (MQ=255)
                         ggCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGAGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTa                                                                                                                         <  7:334280/148‑1 (MQ=255)
                         ggCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGAGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTa                                                                                                                         >  8:334280/1‑148 (MQ=255)
                         ggCGAACGTCACGACCCCTGCAGTCGGTTTAAGGGTGAGGGGGCTGGGTCTTATGGGCGGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCTGGGGTCATTTTTTCGGCTCAGCTTTAc                                                                                                                        <  8:299099/149‑1 (MQ=255)
                          gCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTAcc                                                                                                                       <  1:74214/149‑1 (MQ=255)
                           cGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCg                                                                                                                                                  >  6:229039/1‑121 (MQ=255)
                             aaCGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTgg                                                                                                                    <  2:59069/149‑1 (MQ=255)
                                    cGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAACGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGc                                                                                                             >  5:41393/1‑149 (MQ=255)
                                       ccGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCA‑TTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGt                                                                                                         <  4:124249/149‑1 (MQ=255)
                                                tCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGt                                                                                                         <  8:26004/141‑1 (MQ=255)
                                                tCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGt                                                                                                         >  7:26004/1‑141 (MQ=255)
                                                           ttGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCa                                                                                      <  6:41393/149‑1 (MQ=255)
                                                           ttGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTATGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCa                                                                                      <  2:76736/149‑1 (MQ=255)
                                                                                    gTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTtgtg                                                             >  4:124822/1‑149 (MQ=255)
                                                                                     tAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTgc                                                            <  2:54182/149‑1 (MQ=255)
                                                                                          cgcACGGGCTTGCAGCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGCGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACCGGCTGACCACTTCCTGTTGTGCGCGGt                                                       >  7:307006/1‑149 (MQ=255)
                                                                                           gcACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTc                                                      >  3:51961/1‑149 (MQ=255)
                                                                                                 gCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCa                                                >  8:59026/1‑149 (MQ=255)
                                                                                                   ttGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCAtt                                              >  6:65800/1‑149 (MQ=255)
                                                                                                      cACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCAGACCGCGCacaac                                            <  7:38435/148‑5 (MQ=255)
                                                                                                            aaGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATcac                                      <  6:358182/148‑1 (MQ=255)
                                                                                                                    caggggTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCg                                <  8:210028/143‑1 (MQ=255)
                                                                                                                    cATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATg                             >  8:219869/1‑149 (MQ=255)
                                                                                                                            tgtgATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTgg                     >  8:210858/1‑149 (MQ=255)
                                                                                                                                     aCCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCt                                                     >  1:282626/1‑108 (MQ=255)
                                                                                                                                     aCCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCt                                                     <  2:282626/108‑1 (MQ=255)
                                                                                                                                     aCCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATggg              >  2:14074/1‑147 (MQ=255)
                                                                                                                                          cTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATGGGGAGCTAg       <  5:65800/149‑1 (MQ=255)
                                                                                                                                            cGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATGGGGAGCTAGgt     <  7:210858/149‑1 (MQ=255)
                                                                                                                                               cgcCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATGGGGAGCTAGGTGTa  <  7:219869/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGTCTACCTCCCGGACCAAGGGGAGGGCGAACGTCACGACCGATGCAGTCGGTTTAAGGTTGATGGGGATGGGTCTTATGGGCTGTAAGGCGCACGGGCTTGCACCTTAAGGTCTGCATGGGTGTGTGATCTGACCGGCTCGGCGCCGGGGTCATTTTTTCGGCTCAGCTTTACCTGGTAACCGCTGGTGCTACCCCGGCATACGCCAGACGGGCTGACCACTTCCAGTTGTGCGCGGTCTGCGCATTCAGATCACACACCGATGCAGCCTGGCGATGGGGAGCTAGGTGTA  >  NC_002947/1747717‑1748008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑