Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 182,034 G→A 11.6% intergenic (+23/+42) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947182,0340GA11.6% 151.5 / 9.0 60intergenic (+23/+42)PP_5SB/PP_01605S ribosomal RNA/ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base G (27/26);  new base A (2/5);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

GACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGG  >  NC_002947/181897‑182182
                                                                                                                                         |                                                                                                                                                    
gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGAc                                                                                                                                              <  3:56033/146‑1 (MQ=255)
 acgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAgg                                                                                                                                           <  3:263451/148‑1 (MQ=255)
 acgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAgg                                                                                                                                           <  1:66946/148‑1 (MQ=255)
  cgacCTTAGAGCATTGGAACCACCTGATCCCATGCCGAACTAAGCAGTGAAACGATGCATCGCCGATGGTTGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAggg                                                                                                                                          <  8:121163/148‑1 (MQ=17)
  cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAgggg                                                                                                                                         >  6:152180/1‑149 (MQ=255)
        tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGt                                                                                                                                   <  1:47753/149‑1 (MQ=255)
          gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCt                                                                                                                                 >  5:265532/1‑149 (MQ=255)
                  gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCgg                                                                                                                          <  8:132066/148‑1 (MQ=255)
                  gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGCTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTTTGttttaaggg                                                                                                                         >  7:57085/1‑144 (MQ=255)
                       accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTc                                                                                                                     >  5:359316/1‑148 (MQ=255)
                        ccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTgggt                                                                                                                                 >  1:337748/1‑132 (MQ=255)
                        ccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTgggt                                                                                                                                 <  2:337748/135‑4 (MQ=255)
                               ccatcccGAACTCAGCAGTGAAACTTTGCATCGCCGATGGTTGTGTGGGGTTTCGCCATGTGAGAGTAGGTCATCGTCAAGATTCAAAGCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGt                                                                                                            <  8:57085/149‑1 (MQ=21)
                               ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGt                                                                                                            <  8:3185/149‑1 (MQ=255)
                                cacccggAACTGAGCAGTGAAACGTTGCTTCGCCGATGGTAGTGTGGGGTTTCCCGATGTGAGAGTAGGTCTTGGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt                                                                                                           <  8:26934/143‑1 (MQ=25)
                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc                                                                                                      >  8:117840/1‑149 (MQ=255)
                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGc                                                                                               >  3:2734/1‑149 (MQ=255)
                                                gTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGAc                                                                                           >  7:168426/1‑149 (MQ=255)
                                                       gATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGGTTCATTATCGGTTAGAACTGATAGCTGACCGTGgcg                                                                                    <  5:152180/149‑1 (MQ=255)
                                                        aTGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTACGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGgcgc                                                                                   <  4:49567/149‑1 (MQ=255)
                                                                   aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTgcgc                                                                        <  6:265532/149‑1 (MQ=255)
                                                                    tGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc                                                                                                      >  8:238425/1‑118 (MQ=255)
                                                                    tGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc                                                                                                      <  7:238425/118‑1 (MQ=255)
                                                                       tAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCtt                                                                    <  7:117840/149‑1 (MQ=255)
                                                                         gtgtGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCa                                                                  >  8:184695/1‑149 (MQ=255)
                                                                          tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGctc                                                                      <  4:135260/144‑1 (MQ=255)
                                                                          tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGctc                                                                      >  3:135260/1‑144 (MQ=255)
                                                                          tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAc                                                                 <  4:169898/149‑1 (MQ=255)
                                                                            tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAccc                                                               <  7:184695/149‑1 (MQ=255)
                                                                            tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAcc                                                                >  2:276221/1‑148 (MQ=255)
                                                                            tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAcc                                                                >  2:271588/1‑148 (MQ=255)
                                                                              gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCAt                                                             >  7:117038/1‑149 (MQ=255)
                                                                                    ccccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg                                                         >  6:69097/1‑147 (MQ=255)
                                                                                     cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCa                                                       <  1:276221/148‑1 (MQ=255)
                                                                                     cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCa                                                       <  6:359316/148‑1 (MQ=255)
                                                                                     cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCa                                                       <  1:271588/148‑1 (MQ=255)
                                                                                       cATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGCCCCTGGCGCTCACATTGCGCTCTTCACCCATGTAGCag                                                      >  8:90443/1‑147 (MQ=255)
                                                                                        aTGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagtag                                                   <  8:117038/149‑1 (MQ=255)
                                                                                            gagaGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGtt                                                 >  3:56469/1‑147 (MQ=255)
                                                                                                 tAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTg                                          >  5:249723/1‑149 (MQ=255)
                                                                                                   ggTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCtt                                                                    >  8:249851/1‑121 (MQ=255)
                                                                                                   ggTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCtt                                                                    <  7:249851/121‑1 (MQ=255)
                                                                                                    gTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGca                                       >  5:225960/1‑149 (MQ=255)
                                                                                                          gTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAg                                 >  7:230464/1‑149 (MQ=255)
                                                                                                           tCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAgg                                >  8:229587/1‑149 (MQ=255)
                                                                                                           tCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAgg                                <  4:2734/149‑1 (MQ=255)
                                                                                                           tCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAgg                                >  4:285641/1‑149 (MQ=255)
                                                                                                              aGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTg                                                                                       <  6:170821/91‑1 (MQ=255)
                                                                                                              aGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTg                                                                                       >  5:170821/1‑91 (MQ=255)
                                                                                                              aGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGa                             <  3:285641/149‑1 (MQ=255)
                                                                                                               gATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGAt                            <  6:225960/149‑1 (MQ=255)
                                                                                                                        ccAAAGCCGCTGTCTGCGATGCATACAGGGGCTTTGTCTTTTCCGGGTTTCAATCTCGGTTAGTACTTATAGCTGACCGTGGCGGTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTc                   <  2:303909/149‑1 (MQ=255)
                                                                                                                               cccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCAtt                >  5:352384/1‑145 (MQ=255)
                                                                                                                               cccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCAtt                <  6:352384/145‑1 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt     >  7:82546/1‑149 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt     >  5:90095/1‑149 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt     >  6:61231/1‑149 (MQ=255)
                                                                                                                                      tGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTACTATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt     >  2:173640/1‑149 (MQ=255)
                                                                                                                                        cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgt     <  8:168426/147‑1 (MQ=255)
                                                                                                                                        cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGttgttg   <  5:61231/149‑1 (MQ=255)
                                                                                                                                         gATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt             >  5:140595/1‑138 (MQ=255)
                                                                                                                                         gATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt             <  6:140595/138‑1 (MQ=255)
                                                                                                                                         gATGCAGACAGGGGCTTTGTCTTTTCCGGGTTACTATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTgg  <  1:173640/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
GACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGG  >  NC_002947/181897‑182182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑