Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,898,889	T→A	13.7%	noncoding (36/77 nt)	PP_t18 →	tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,898,889	0	T	A	13.7%	58.9 / 2.8	29	noncoding (36/77 nt)	PP_t18	tRNA‑Asp
Reads supporting (aligned to +/- strand):  ref base T (15/10);  new base A (2/2);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCCGTCATCACCCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCA  >  NC_002947/1898822‑1899032                                                                    |                                                                                                                                                aTCCCGTCATCACCCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTaaa                                                                 >  6:274292/1‑148 (MQ=11)      gTCATCACCCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAaaaga                                                            >  4:212758/1‑148 (MQ=32)       tcatcaCCCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAaaagaaa                                                          <  1:132190/149‑1 (MQ=32)              ccaccaCTCGATGTATAGCGCAGCAGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCt                                                   >  3:155764/1‑149 (MQ=17)              ccaccaCTCGATATATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCt                                                   >  5:39331/1‑149 (MQ=17)                accacTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCt                                                   >  3:211402/1‑147 (MQ=32)                  cacTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAgg                                               >  6:92299/1‑149 (MQ=32)                     tCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACAGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAAAACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTc                                            >  6:332445/1‑149 (MQ=21)                       gATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGc                                          >  2:85305/1‑149 (MQ=34)                          gTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCttt                                       >  4:146003/1‑149 (MQ=34)                            ataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCttttt                                     >  5:14280/1‑149 (MQ=34)                            ataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCttttt                                     >  3:1988/1‑149 (MQ=34)                             taGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCttttt                                     <  4:211402/148‑1 (MQ=34)                               gcccAGCGGTAGTTCAGTCGGTAAAAATACCGGCCTGAAACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTCCGCAATATTCCACTACCAGGGCCCACTCAACACCCGCAAGTAAAAAACAAAGCGACCTTAGGGTCGCttttttt                                   <  2:358069/145‑1 (MQ=11)                                    gCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCt                             <  1:85305/149‑1 (MQ=39)                                     cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTg                            <  3:212758/149‑1 (MQ=40)                                       gTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGca                          >  2:49053/1‑149 (MQ=255)                                            tCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGcatcat                      <  6:14280/148‑1 (MQ=255)                                                   gTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGt              >  3:14232/1‑149 (MQ=255)                                                       gAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTAc          <  5:332445/149‑1 (MQ=255)                                                          tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGcc       >  7:99685/1‑149 (MQ=255)                                                          ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTAttt                               >  1:291098/3‑125 (MQ=255)                                                          ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTAttt                               <  2:291098/123‑1 (MQ=255)                                                           cccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGccc      >  6:351555/2‑149 (MQ=255)                                                            ccGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCt     <  4:155764/149‑1 (MQ=255)                                                            ccGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCt     <  6:39331/149‑1 (MQ=255)                                                              ggCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCtac   <  8:99685/149‑3 (MQ=255)                                                               gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGca  <  4:24726/149‑1 (MQ=255)                                                               gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGc   >  3:24726/1‑148 (MQ=255)                                                                    |                                                                                                                                                ATCCCGTCATCACCCACCACTCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTTTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCA  >  NC_002947/1898822‑1899032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑