Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,984,435 T→C 8.0% V209A (GTG→GCG)  PP_1776 → bifunctional mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,984,4350TC 8.0% 138.7 / 2.7 50V209A (GTG→GCG) PP_1776bifunctional mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (21/25);  new base C (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATCGAGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAGCGTGCCGGACATCTCCATC  >  NC_002947/1984287‑1984580
                                                                                                                                                    |                                                                                                                                                 
aTCGAGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACtgt                                                                                                                                                   <  3:262661/149‑1 (MQ=255)
aTCGAGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACACCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACtgt                                                                                                                                                   >  1:150719/1‑149 (MQ=255)
  cGAGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACtgtg                                                                                                                                                  >  6:307032/1‑148 (MQ=255)
    aGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATc                                                                                                                                               <  2:6619/149‑1 (MQ=255)
      gCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATc                                                                                                                                               >  1:105796/1‑147 (MQ=255)
      gCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGa                                                                                                                                             <  6:216840/149‑1 (MQ=255)
      gCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGa                                                                                                                                             <  2:105796/149‑1 (MQ=255)
       cTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAg                                                                                                                                            <  5:307032/149‑1 (MQ=255)
          cGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCa                                                                                                                                          <  4:283708/148‑1 (MQ=255)
            gCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGtt                                                                                                                                       <  3:62935/149‑1 (MQ=255)
            gCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGtt                                                                                                                                       <  7:49132/149‑1 (MQ=255)
              tGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGtt                                                                                                                                       >  5:371434/1‑147 (MQ=255)
                         tCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCt                                                                                                                          >  1:318846/1‑149 (MQ=255)
                           gAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTcc                                                                                                                        >  4:271231/1‑149 (MQ=255)
                           gAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTc                                                                                                                         <  6:38575/148‑1 (MQ=255)
                               gTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGAt                                                                                                                    <  2:134383/149‑1 (MQ=255)
                                     tAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCtt                                                                                                              >  5:76664/1‑149 (MQ=255)
                                       agagTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAg                                                                                                                                            <  7:268400/117‑1 (MQ=255)
                                       agagTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAg                                                                                                                                            >  8:268400/1‑117 (MQ=255)
                                       agagTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCtt                                                                                                              <  1:219864/147‑1 (MQ=255)
                                       agagTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTg                                                                                                             >  7:294518/1‑148 (MQ=255)
                                        gagTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGaa                                                                                                           <  2:318846/149‑1 (MQ=255)
                                        gagTCGAGCGTTTCGTCGAAAAACCCGACACCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGaa                                                                                                           <  2:150719/149‑1 (MQ=255)
                                                  tttCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGc                                                                                                 >  2:21888/1‑149 (MQ=255)
                                                      gtcgAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCt                                                                                                                       >  2:10895/1‑123 (MQ=255)
                                                      gtcgAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCt                                                                                                                       <  1:10895/123‑1 (MQ=255)
                                                       tcgCAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTAcc                                                                                            <  8:294518/149‑1 (MQ=255)
                                                       tcgAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTAc                                                                                             >  7:166196/1‑148 (MQ=255)
                                                        cgAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTAccc                                                                                           >  7:114106/1‑149 (MQ=255)
                                                         gAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTAccc                                                                                           <  8:114106/148‑1 (MQ=255)
                                                             aaCCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGCGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCg                                                                                        >  6:315814/1‑147 (MQ=255)
                                                             aaCCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGCGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCg                                                                                        >  6:309913/1‑147 (MQ=255)
                                                                       cAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGcgcc                                                                            >  8:199908/1‑149 (MQ=255)
                                                                                  aGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCgcgc                                                                             <  2:261200/137‑1 (MQ=255)
                                                                                  aGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCgcgc                                                                             >  1:261200/1‑137 (MQ=255)
                                                                                      cTATGTCGCTGCTGGAAACTATTTCAGCAACGCCCGCATGTTATGCTACCAGCTTGCCACTCTGAAGGAGCAGTTCAGGGATTACGCTCCTCAAGTCCTTGAAGCTTTCGAGCGTACCCTCGAAGCCTAGCGCCGCACTACCTCAAAAg                                                             >  5:340371/1‑149 (MQ=255)
                                                                                             gctgctGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGATCAGTTCAGGGCTTACGCTCCTGATGTGCTTGATGCTGTCGAGCGTAACCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCtata                                                       <  6:76664/148‑1 (MQ=255)
                                                                                                 ctgGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTAc                                                                     >  3:89610/1‑130 (MQ=255)
                                                                                                 ctgGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTAc                                                                     <  4:89610/130‑1 (MQ=255)
                                                                                                  tgGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGcc                                                 >  4:113168/1‑149 (MQ=255)
                                                                                                    gCAACTATTTCTGGAACGCCGGCATGTTCTTCTTCCAGTTTGGCACAGTGATCAAGCATTTCAGGGCTTACGCTCCTGAGGTCATTGAAGCTGTCGAGCGTACCCTCGACGACTCGCGCCGCTGGACATCCAAAGGCTATAGCTGCCTg                                               >  2:166955/1‑149 (MQ=255)
                                                                                                      aaCTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGc                                                                                                 >  8:157810/1‑97 (MQ=255)
                                                                                                      aaCTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGc                                                                                                 <  7:157810/97‑1 (MQ=255)
                                                                                                           tttCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGCGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGctct                                          <  5:315814/147‑1 (MQ=255)
                                                                                                           tttCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACAGCGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGctct                                          <  5:309913/147‑1 (MQ=255)
                                                                                                            ttCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGa                                       >  1:262156/1‑149 (MQ=255)
                                                                                                                 gAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGTGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTTCCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCa                                   <  1:21888/148‑1 (MQ=255)
                                                                                                                   aCGCCGGCATGTTCTGCTTCCAGGTTGGGACTGTGATCGAGCAGTTAATGGCTTTCGCTCCTTATTTGGGTGAAGCTGTGGAGCGTACCCTCGAAGCCTCGCGCCGGTCTTCGTCCAAAGTGTATAGCTGCCTGGCTCTGGTTTCagag                                <  6:340371/149‑1 (MQ=255)
                                                                                                                              ttCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGt                               >  6:163420/1‑139 (MQ=255)
                                                                                                                              ttCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGt                               <  5:163420/139‑1 (MQ=255)
                                                                                                                              ttCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAg                     >  4:37587/1‑149 (MQ=255)
                                                                                                                                      ccAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAGCGTGCCg              <  2:262156/148‑1 (MQ=255)
                                                                                                                                          gTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAGCGTGCCGGACAt         <  3:37587/149‑1 (MQ=255)
                                                                                                                                                  tgtgATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAGCGTGCCGGACATCTCCATc  >  5:327930/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATCGAGGCTGCGGCTGGCGGTGTGCTCGAAGGTGGGCTAAGAGTCGAGCGTTTCGTCGAAAAACCCGACGCCAAAACCGCTGAGGCCTATGTCGCTGCTGGCAACTATTTCTGGAACGCCGGCATGTTCTGCTTCCAGGTTGGCACTGTGATCGAGCAGTTCAGGGCTTACGCTCCTGATGTGCTTGAAGCTGTCGAGCGTACCCTCGAAGCCTCGCGCCGCTCTACCTCCAAAGGCTATAGCTGCCTGGCTCTGGATGCAGAGTGCTTCGCCAGCGTGCCGGACATCTCCATC  >  NC_002947/1984287‑1984580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑